Journal: Research in Pharmaceutical Sciences

Article Title: Anti- Toxoplasma gondii activity of 5-oxo-hexahydroquinoline derivatives: synthesis, in vitro and in vivo evaluations, and molecular docking analysis

doi: 10.4103/1735-5362.293515

Figure Lengend Snippet: Flow cytometry analysis of Toxoplasma gondii tachyzoites exposed to different concentrations of Z1, Z2, Z3, Z4, negative and positive controls.

Article Snippet: The cell suspension was transferred into polystyrene flow cytometry tubes (BD Falcon, USA).

Techniques: Flow Cytometry

Journal: Nuclear medicine and biology

Article Title: A Method to Predict Response of Cell Populations to Cocktails of Chemotherapeutics and Radiopharmaceuticals: Validation with Daunomycin, Doxorubicin, and the Alpha Particle Emitter 210 Po

doi: 10.1016/j.nucmedbio.2012.01.011

Figure Lengend Snippet: Uncompensated flow cytometry dot plots for control samples consisting of cells treated with A) 0.63 μM daunomycin, B) 0.63 μM daunomycin + 3 mM citrate, C) untreated, and D) 3 mM citrate,

Article Snippet: To detect citrate, cells were resuspended in 1 mL of MOPS buffer containing 100 μM of europium tetracycline (Sigma, cat #s 203254 for europium and T7660 for tetracycline), transferred into 7-mL polystyrene flow cytometry tubes (BD, cat # 352054), and incubated at room temperature (~22°C) in the dark for 30 min.

Techniques: Flow Cytometry

Journal: Nuclear medicine and biology

Article Title: A Method to Predict Response of Cell Populations to Cocktails of Chemotherapeutics and Radiopharmaceuticals: Validation with Daunomycin, Doxorubicin, and the Alpha Particle Emitter 210 Po

doi: 10.1016/j.nucmedbio.2012.01.011

Figure Lengend Snippet: Compensated flow cytometry dot plots for control samples consisting of cells treated with A) 0.63 μM daunomycin, B) 0.63 μM daunomycin + 3 mM citrate, C) untreated, and D) 3 mM citrate, FlowJo software was used to compensate for overlapping emission spectra.

Article Snippet: To detect citrate, cells were resuspended in 1 mL of MOPS buffer containing 100 μM of europium tetracycline (Sigma, cat #s 203254 for europium and T7660 for tetracycline), transferred into 7-mL polystyrene flow cytometry tubes (BD, cat # 352054), and incubated at room temperature (~22°C) in the dark for 30 min.

Techniques: Flow Cytometry, Software

Journal: Nuclear medicine and biology

Article Title: A Method to Predict Response of Cell Populations to Cocktails of Chemotherapeutics and Radiopharmaceuticals: Validation with Daunomycin, Doxorubicin, and the Alpha Particle Emitter 210 Po

doi: 10.1016/j.nucmedbio.2012.01.011

Figure Lengend Snippet: Flow chart of Monte Carlo procedure for determining fraction of surviving cells based on cellular fluorescence intensity distributions that arise after treatment of cells with a cocktail of two therapy agents (Agent 1 and Agent 2). In STEP 1, flow cytometry is used to obtain a dot-plot of the treated cells. STEP 2 calculates the probability P=(Ii1′,Ii2′) that the ith cell survives treatment with Agents 1 and 2, based on the respective normalized fluorescence intensities Ii1′ and Ii2′. A random number RANDi (0 < RANDi ≤ 1) is then generated as depicted in STEP 3 by the dice. If RSNDi< P(Ii1′,Ii2′), then the cell is scored as a survivor, otherwise it is considered dead (STEP 4). By repeating STEPS 1–4 for every cell, the surviving fraction for any sample population is calculated as illustrated in STEP 5.

Article Snippet: To detect citrate, cells were resuspended in 1 mL of MOPS buffer containing 100 μM of europium tetracycline (Sigma, cat #s 203254 for europium and T7660 for tetracycline), transferred into 7-mL polystyrene flow cytometry tubes (BD, cat # 352054), and incubated at room temperature (~22°C) in the dark for 30 min.

Techniques: Fluorescence, Flow Cytometry, Generated