Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine increases the mucin 5AC (MUC5AC) level and inhibits the expression of caudal type homeobox 2, MUC2, and Trefoil factor family 3 in Atp4a -/- mice. A: Expression of caudal type homeobox 2 (CDX2), mucin 2 (MUC2), Trefoil factor family 3 (TFF3) and MUC5AC in Atp4a -/- mouse gastric tissues was assessed via immunohistochemistry (IHC) staining ( n = 6); B: IHC scores of CDX2, MUC2, and TFF3 ( n = 6); C: IHC score of MUC5AC ( n = 6). a P < 0.01 vs Atp4a -/- ; b P < 0.01 vs wild-type (WT).

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Immunohistochemistry

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine downregulates the protein expression of caudal type homeobox 2 and Trefoil factor family 3 in gastric intestinal metaplasia control organoids. A-C: Western blotting was conducted to assess the protein levels of caudal type homeobox 2 (CDX2) and Trefoil factor family 3 (TFF3); D-I: Relative protein levels were quantified. a P < 0.01 vs normal control; b P < 0.05 vs gastric intestinal metaplasia (GIM) control (CON); c P < 0.01 vs GIM CON.

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Control, Western Blot

Journal: Journal of sport and health science

Article Title: BMAL1/PGC1α4-FNDC5/irisin axis impacts distinct outcomes of time-of-day resistance exercise.

doi: 10.1016/j.jshs.2024.100968

Figure Lengend Snippet: Fig. 7. Irisin increased muscle hypertrophy via aV class of integrins and FAK-AKT-CREB signaling. Representative western blot analysis and quantification of (A) integrin-like signaling FAK-AKT-CREB phosphorylation and (B) Quantitative Polymerase Chain Reaction (qPCR) analysis in differentiated C2C12 myotubes pre-treated with negative control RGD (Con) or RGDS (10 uM) for 10 min and then treated with recombinant irisin (600 ng/mL) or His-tag (600 ng/mL). Repre- sentative Western blot analysis and quantification of (C) integrin-like signaling FAK-AKT-CREB phosphorylation and (D) qPCR analysis of IL6 in gastrocnemius muscle (GAS) from mice i.p. injected with His-tag or recombinant irisin (1mg/kg) and treated with negative control RGD (Con) or cRGDyK (1mg/kg) peptide every day for 2 weeks. (E) Mice were treated with integrin aV inhibitor cRGDyK (1 mg/kg) or negative control RGD (Con) for 48 h and their grip strength was assessed at late rest (LR) or late active (LA) phase. n = 6 per group. Percentage of (F) body weight change, (G) representative Hematoxylin and Eosin (H&E) staining, and (H) quantification of average fiber sizes of GAS muscles from mice i.p. injected with His-tag or recombinant irisin (1 mg/kg) in the combination of negative control RGD peptide (Con) or cRGDyK (1 mg/kg) every day for 2 weeks. n = 4 per group. Scale bar = 100 um. All data were shown as mean § SEM. Two-tailed unpaired Student’s t test was used for statistical comparison. *p < 0.05; ** p < 0.01. C2C12 = C2 myoblast cell line 12; GAPDH = Glyceraldehyde-3- phosphate Dehydrogenase; ns = not significant; RGDS = Arg-Gly-Asp-Ser.

Article Snippet: For quantitative real-time-polymerase chain reaction (qRT-PCR) and western blot analysis, differentiated C2C12 myotubes were pre-treated with 10 mM negative control Arginyl-glycyl-aspartic acid (RGD) (GL Biochem Chemicals, Shanghai, China) or RGDS (S8008; Selleck Chemicals, Houston, TX, USA) and administrated with recombinant irisin or His-tag at 600 ng/mL for indicated time.

Techniques: Western Blot, Phospho-proteomics, Real-time Polymerase Chain Reaction, Negative Control, Recombinant, Injection, Staining, Muscles, Two Tailed Test, Comparison