Journal: Frontiers in Physiology

Article Title: Pathogenic gain-of-function mutations in the prodomain and C-terminal domain of PCSK9 inhibit LDL binding

doi: 10.3389/fphys.2022.960272

Figure Lengend Snippet: Ser-127 residue is critical to LDL binding function of PCSK9. (A) 24 h post-transfection, HEK293 cells transiently expressing WT-PCSK9 or indicated S127 mutants were harvested and cell lysates and medium immunoprecipitates were analyzed by 8% SDS-PAGE and western blotting to detect precursor (P) and mature (M) forms of PCSK9. # indicates a faster-migrating form of PCSK9 consistent with furin-mediated proteolysis. Shown is a representative experiment ( n = 3) (B) Mouse Hepa-1c1c7 cells cultured in sterol-depleting medium were treated for 4 h with WT or indicated mutant forms of PCSK9 (2.5 μg/ml). Biotinylated cell surface LDLRs were isolated and quantified by Western blotting using transferrin receptor (TfR) as a loading control. Shown are representative western blots ( top ) with densitometric analysis of three independent experiments ( bottom ). Error bars represent SEM ( n = 3). Significant change in LDLR expression compared to WT-PCSK9 was determined by Student’s t -test: ** p < 0.01. (C) Conditioned cell culture medium containing WT-PCSK9 (WT) or indicated mutants were incubated with LDL prior to density gradient-ultracentrifugation and immunoprecipitation and Western blot analysis of LDL-containing fractions. Shown is a representative experiment ( n = 3)

Article Snippet: We obtained fetal bovine serum (FBS) and newborn calf serum from ThermoFisher, EDTA-free Complete™ Protease Inhibitor Tablets were from Roche, Optiprep™ density gradient medium (60% w/v iodixanol) from Axis-Shield, NP-40 detergent was from Biovision and PolyJet DNA transfection reagent was from FroggaBio (Toronto, Ontario).

Techniques: Binding Assay, Transfection, Expressing, SDS Page, Western Blot, Cell Culture, Mutagenesis, Isolation, Incubation, Immunoprecipitation

Journal: Journal of Digital Imaging

Article Title: Imaging Properties of Additive Manufactured (3D Printed) Materials for Potential Use for Phantom Models

doi: 10.1007/s10278-019-00257-5

Figure Lengend Snippet: Machine details. Details of each printing style used in this study

Article Snippet: Contains the sample part map and images of the phantom under ultrasound, CT, and MRI for the Printer Style phantom table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Machine details Machine Company Resolution Materials SLA Projet 6000HD 3D Systems Rock Hill, USA 0.004 in. (0.1 mm) VisoClear FDM Fortus 450mc Stratasys, Ltd Eden Prairie, USA 0.005 in. (0.13 mm) ABS ASA PolyJet Connex 500 Objet/Stratasys, Ltd Eden Prairie, USA 28 μm (0.0011 in.)

Techniques:

Journal: Advanced Science

Article Title: Multi‐Material 3D and 4D Printing: A Survey

doi: 10.1002/advs.201902307

Figure Lengend Snippet: Additive manufacturing technologies

Article Snippet: PolyJet (Stratasys Ltd., USA) is probably the most common commercially available multi‐material jetting process.

Techniques: Transformation Assay, Functional Assay, Injection, Selection, Adhesive