Journal: Oncology Reports

Article Title: Dexamethasone induces aberrant macrophage immune function and apoptosis

doi: 10.3892/or.2019.7434

Figure Lengend Snippet: Effects of KLF9 on the production of proinflammatory cytokines from LPS-induced RAW264.7 cells. (A) RAW264.7 cells were transfected with Lenti-KLF9 and then co-treated with 1 µg/ml LPS for 24 h. The mRNA levels of TNF-α, IL-1β, IL-6 are analyzed by Real-time quantitative PCR analysis. (B) An ELISA assay was used to determine TNF-α, IL-1β, IL-6 production in the supernatants of RAW264.7 cells following treatment with 1 µg/ml LPS and transfected with Lenti-KLF9 or control, respectively. (C) Proliferation rate of HepG2 cells was assessed using CCK8 after co-culture with RAW264.7 cells following treatment with 1 µg/ml LPS and transfected with Lenti-KLF9 or control, respectively. (D) ELISA assay was used to determine PGE2 production in the supernatants of RAW264.7 cells following treatment with 1 µg/ml LPS and transfected with Lenti-KLF9 or control, respectively. Experiments were performed in triplicate. Error bars represent the standard deviation (*P<0.05; **P<0.01; ***P<0.005; ****P<0.001). KLF9, Krüppel-like factor 9; LPS, lipopolysaccharide.

Article Snippet: ELISA kits were purchased from R&D Systems for IL-1β, IL-6 and TNF-α (product no. A54609) and PGE2 (product no. A50432) was purchased from EpiGentek.

Techniques: Transfection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control, Co-Culture Assay, Standard Deviation