Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Transmission electron microscopy of carotid arteries of WT and PAI-1−/− mice 3 days after cuff implantation. A: A neutrophil with vesicular transport activity (arrow) is seen attached to the luminal endothelium in WT carotid artery close to a cellular junction (asterisk) (original magnification, ×11,500). B: A polymorphonuclear leukocyte (arrow) is present in an open subendothelial space in WT carotid artery (original magnification, ×6,200). A and B suggest that a diapedesis process is occurring. C: Altered cellular morphology of an endothelial cell (E) is evident in PAI-1−/− carotid artery, the result of copper-induced injury (original magnification, ×6,800). D: Activated macrophages with visible phagolysosomes (P) are evident in the medial compartment of a PAI-1−/− carotid artery (original magnification, ×2,700).

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques: Transmission Assay, Electron Microscopy, Activity Assay

Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Histological analysis of carotid arteries from WT and PAI-1−/− mice 7 days after implantation of the copper cuff. A: H&E stain of WT carotid artery demonstrates a reactive adventitial (asterisk) and medial (arrow) compartments with the presence of a small neointima (arrowhead). Original magnification, ×200. Similar H&E staining of a carotid artery from a PAI-1−/− mouse (B) shows a quiescent state of these vascular compartments. Original magnification, ×200. C: Anti-α-actin immunostain of a WT carotid artery demonstrates enhanced smooth muscle cells in the medial compartment (arrow) (original magnification, ×200), whereas a PAI-1−/− carotid artery (D) indicates no apparent change in the smooth muscle cell population in the medial compartment. Original magnification, ×200. E: CD45 immunostain of WT carotid artery demonstrates a leukocyte-enriched adventitia (arrow). Original magnification, ×200. F: CD45 immunostain of WT carotid artery demonstrates leukocytes within the neointima (arrow). Original magnification, ×400. G: Anti-fibrin(ogen) immunostain of WT carotid artery shows fibrin-rich medial (arrow) and adventitial (asterisk) compartments (original magnification, ×200), whereas similar immunostaining of a PAI-1−/− carotid artery (H) demonstrates no apparent fibrin deposition in the vessel wall. Original magnification, ×200.

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques: Staining, Immunostaining

Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Vessel counts per mm 2 area of the adventitial compartment of arteries from WT and PAI-1−/− mice 7 and 21 days after perivascular placement of the cuff. WT versus PAI-1−/− at day 21, P = 0.0057.

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques:

Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. In all cases, the particles had been incubated in three different buffers, i.e., serum-free HBSS (“plain”), HBSS with 10% untreated autologous serum (37°C-ASPO), and HBSS with 10% autologous serum that had been heat-treated at 56°C (56°C-ASPO). The legend included in (A) applies to all panels.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Incubation

Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. The first three columns depict example data obtained with sera from different, healthy donors. The rightmost column summarizes the results for a total of 3 donors. Values are block-centered means ± SD.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Blocking Assay