polyclonal anti mouse igg Search Results


horseradish peroxidase conjugated rabbit anti mouse  (OriGene)
94
OriGene horseradish peroxidase conjugated rabbit anti mouse
Horseradish Peroxidase Conjugated Rabbit Anti Mouse, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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mouse igg antibodies  (Cedarlane)
93
Cedarlane mouse igg antibodies
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Mouse Igg Antibodies, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
mouse igg antibodies - by Bioz Stars, 2026-10
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rabbit anti mouse il 1a polyclonal serum  (Cedarlane)
93
Cedarlane rabbit anti mouse il 1a polyclonal serum
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Rabbit Anti Mouse Il 1a Polyclonal Serum, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+mouse+igg/Anti-Mouse+Serum%2C+IgG+Fraction%2C+(Polyclonal)+(rabbit+IgG)/pm09830023-69-12-17
Average 93 stars, based on 1 article reviews
rabbit anti mouse il 1a polyclonal serum - by Bioz Stars, 2026-10
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goat anti mouse hpr igg  (OriGene)
94
OriGene goat anti mouse hpr igg
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Goat Anti Mouse Hpr Igg, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
goat anti mouse hpr igg - by Bioz Stars, 2026-10
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anti mouse rabbit igg detection system  (OriGene)
93
OriGene anti mouse rabbit igg detection system
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Anti Mouse Rabbit Igg Detection System, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+mouse+igg/Mouse+IgG+(F(ab)2+specific)+Rabbit+Polyclonal+Antibody/pm40119128-193-8-12
Average 93 stars, based on 1 article reviews
anti mouse rabbit igg detection system - by Bioz Stars, 2026-10
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rabbit anti mouse pmn antibody  (Cedarlane)
93
Cedarlane rabbit anti mouse pmn antibody
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Rabbit Anti Mouse Pmn Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
rabbit anti mouse pmn antibody - by Bioz Stars, 2026-10
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tetramethylrhodamine conjugated goat anti mouse igg  (OriGene)
90
OriGene tetramethylrhodamine conjugated goat anti mouse igg
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Tetramethylrhodamine Conjugated Goat Anti Mouse Igg, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
tetramethylrhodamine conjugated goat anti mouse igg - by Bioz Stars, 2026-10
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hrp conjugated goat anti mouse igg  (OriGene)
91
OriGene hrp conjugated goat anti mouse igg
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Hrp Conjugated Goat Anti Mouse Igg, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp conjugated goat anti mouse igg - by Bioz Stars, 2026-10
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rabbit anti mouse coli  (Cedarlane)
93
Cedarlane rabbit anti mouse coli
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Rabbit Anti Mouse Coli, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
rabbit anti mouse coli - by Bioz Stars, 2026-10
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rabbit anti mouse laminin  (Cedarlane)
91
Cedarlane rabbit anti mouse laminin
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Rabbit Anti Mouse Laminin, supplied by Cedarlane, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti mouse igg hrp  (Cedarlane)
93
Cedarlane rabbit anti mouse igg hrp
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Rabbit Anti Mouse Igg Hrp, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tetramethylrhodamine isothiocyanate tritc conjugated goat anti mouse igg  (OriGene)
90
OriGene tetramethylrhodamine isothiocyanate tritc conjugated goat anti mouse igg
The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse <t>IgG</t> Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).
Tetramethylrhodamine Isothiocyanate Tritc Conjugated Goat Anti Mouse Igg, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse IgG Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).

Journal:

Article Title: Expansion of a CD28-Intermediate Subset among CD8 T Cells in Patients with Infectious Mononucleosis

doi: 10.1128/JVI.76.13.6602-6608.2002

Figure Lengend Snippet: The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse IgG Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).

Article Snippet: For blocking experiments, an anti-class I MAb (Clone W6/32; Cederlane, Hornby, Ontario, Canada) or isotype-matched mouse IgG antibodies were used at a final concentration of 50 μg/ml.

Techniques: Activity Assay, Transformation Assay, Incubation, Isolation, Labeling, Radioactivity, Lysis, Inhibition