Journal: Journal of Family & Reproductive Health

Article Title: Effect of Lysophosphatidic Acid on the Vascular Endothelial Growth Factor Expression in Autotransplanted Mouse Ovaries Encapsulated in Sodium Alginate

doi: 10.18502/jfrh.v15i2.6449

Figure Lengend Snippet: The sequences of the designed primers

Article Snippet: The cells were permeable by putting the sections in triton X100 (0.3 % for 30 min), washed in PBS and blocked with goat serum (30 min) then, they were incubated with the primary anti-VEGF polyclonal antibody (1:100, Elabscience Biotechnology Co, Wuhan, China) overnight.

Techniques: Sequencing

Journal: Journal of Family & Reproductive Health

Article Title: Effect of Lysophosphatidic Acid on the Vascular Endothelial Growth Factor Expression in Autotransplanted Mouse Ovaries Encapsulated in Sodium Alginate

doi: 10.18502/jfrh.v15i2.6449

Figure Lengend Snippet: The fluorescent microscopy observations of transplanted mouse ovarian tissue sections immunostained for VEGF antibody (first row) and phase contrast of the same groups in second row. A and a: Intact control group; B and b: LPA+/LPA-; C and c: LPA-/LPA-. Green color shows the positive cell reaction (white arrow) for VEGF antibody (Bar=50μm). The comparison of the relative expression ratio of Vegf gene to β- actin in transplanted mouse ovaries and intact control group are shown in parts D.

Article Snippet: The cells were permeable by putting the sections in triton X100 (0.3 % for 30 min), washed in PBS and blocked with goat serum (30 min) then, they were incubated with the primary anti-VEGF polyclonal antibody (1:100, Elabscience Biotechnology Co, Wuhan, China) overnight.

Techniques: Microscopy, Control, Comparison, Expressing

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Differential Proteomic Analysis of Gender-dependent Hepatic Tumorigenesis in Hras12V Transgenic Mice *

doi: 10.1074/mcp.M116.065474

Figure Lengend Snippet: Validation of the differentially expressed proteins that were identified by MALDI-TOF/TOF-MS. Seven proteins (HSP90B1, beta-actin, albumin, APOA1, CALR, FABP5, and PRDX6) were randomly selected from the differently expressed proteins that were identified by MALDI-TOF/TOF-MS and were validated in transgenic and nontransgenic males (A) and females (B) by Western blot assay. Wt: the liver tissue of wild-type mice; P: the peritumor tissue of transgenic mice; T: the tumor tissue of transgenic mice; Non-Tg, C57BL/6J wild-type nontransgenic mice; Tg: transgenic mice. The numbers indicate different individuals. Bradford reagent was used as the loading control.

Article Snippet: Primary and secondary antibodies: anti-phoshpo-MEK-1/2 polyclonal antibody (diluted 1:1000, Elabscience, Wuhan, China), anti-phoshpo-ERK1/2 monoclonal antibody (diluted 1:2000, Cell Signaling Technology, MA), anti-phoshpo-PI3 Kinase polyclonal antibody (diluted 1:1000, Cell Signaling Technology), anti-phoshpo-Akt monoclonal antibody (diluted 1:2000, Cell Signaling Technology), anti-phoshpo-mTOR monoclonal antibody (diluted 1:1000, Cell Signaling Technology), anti-APOA1 monoclonal antibody (diluted 1:1000, Proteintech, Wuhan, China), anti-β-actin polyclonal antibody (diluted 1:5000, Bioworld Technology Co., Ltd., MN), anti-HSP90B1 polyclonal antibody (diluted 1:1000, Elabscience), anti-albumin polyclonal antibody (diluted 1:1000, Abclonal, Boston, MA), anti-calreticulin polyclonal antibody (diluted 1:1000, Abways, Shanghai, China), anti-fatty acid binding protein 5 polyclonal antibody (diluted 1:500, Proteintech), anti-peroxiredoxin VI monoclonal antibody (diluted 1:2000, Abfrontier, Santiago, CA), anti-mouse-IgG antibody (diluted 1:5000, Bioworld) and anti-rabbit-IgG antibody (diluted 1:5000, Bioworld).

Techniques: Biomarker Discovery, Transgenic Assay, Western Blot, Control

Journal: Autophagy

Article Title: Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model

doi: 10.1080/15548627.2019.1596488

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: Rabbit polyclonal anti-SQSTM1 , Elabscience , EAP3350 , 1:1000 , - , -.

Techniques: Western Blot, Immunofluorescence, Immunohistochemistry