Journal: bioRxiv

Article Title: Caerin 1.1 and 1.9 peptides from Australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model

doi: 10.1101/2021.04.20.440726

Figure Lengend Snippet: Pus resulted from MRSA infected murine skin was mixed with different concentrations of caerin gel (12.5 6.25, 3.125, and 1.5625 mg/ml) and control poloxamer gel, then 30ul of the mixture was put onto a nutrition agar plate and incubated at 37°C for 24 hours. Each bar represented the number of countable bacterial colonies at certain concentration, and the error bar represented the standard deviations. ***P<0.001; ns, not significant.

Article Snippet: The lipopolysaccharide concentration of caerin 1.1, caerin 1.9 and control peptide (P3) was less than 0.44EU/ml as measured by Kinetic Turbidimetric Assay by Xiamen Bioendo Technology Co., Ltd. Poloxamer 407 (molecular weight 12600, batch number WPAK592B) and poloxamer 188 (molecular weight 8400, batch number WPAK539B) were purchased from Badische Anilin-und-Soda-Fabrik (BASF; Ludwigshafen, Germany).

Techniques: Infection, Incubation, Concentration Assay

Journal: bioRxiv

Article Title: Caerin 1.1 and 1.9 peptides from Australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model

doi: 10.1101/2021.04.20.440726

Figure Lengend Snippet: Balb/c mice with severe MRSA skin infection, and the pair comparisons of bacteria counts between caerin gel and control gel. Approximately 5×10 6 CFU were inocultured on each wound; with 12.5mg/ml of caerin 1.1 (F1) and caerin 1.9 (F3) peptides in poloxamer gel, or with gel only for three days. Four mice were included in this group. The caerin gel inhibited the growth of MRSA at a significant level of p<0.05.

Article Snippet: The lipopolysaccharide concentration of caerin 1.1, caerin 1.9 and control peptide (P3) was less than 0.44EU/ml as measured by Kinetic Turbidimetric Assay by Xiamen Bioendo Technology Co., Ltd. Poloxamer 407 (molecular weight 12600, batch number WPAK592B) and poloxamer 188 (molecular weight 8400, batch number WPAK539B) were purchased from Badische Anilin-und-Soda-Fabrik (BASF; Ludwigshafen, Germany).

Techniques: Infection

Journal: bioRxiv

Article Title: Caerin 1.1 and 1.9 peptides from Australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model

doi: 10.1101/2021.04.20.440726

Figure Lengend Snippet: Tape-stripped Babl/c mice and C57BL/6 mice were infected with MRSA. Comparisons of bacteria counts between caerin gel (combined caerin 1.1 and caerin 1.9) and control gel, between P3 gel and control gel, and between two sides in the normal saline control group. Approximately 5×10 4 CFU were inocultured on each wound; with 12.5mg/ml of caerin 1.1 and caerin 1.9 peptides in poloxamer gel, or with gel only for three days. Each line represented the pair comparison of the number of bacteria (CFU) between the right and left stripped areas per mice. A . The pair comparison of the CFU between caerin gel and control gel. B . The pair comparison of the CFU between P3 gel and control gel. C . The pair comparison of the CFU between two sides of normal saline. D . The pair comparison of the CFU between caerin gel and control gel. E . The pair comparison of the CFU between P3 gel and control gel. F . The pair comparison of the CFU between two sides of normal saline. The number of mice in each group was as followed: A, n=7; B, n=6; C, n=5; D, n=6; E, n=6; F, n=6. The result is the representative of two independent experiments. Wilcoxon matched-pairs test in the GraphPad Prism 7 software was used to analyze data. The significance was determined by the p-value at the level of 0.05.

Article Snippet: The lipopolysaccharide concentration of caerin 1.1, caerin 1.9 and control peptide (P3) was less than 0.44EU/ml as measured by Kinetic Turbidimetric Assay by Xiamen Bioendo Technology Co., Ltd. Poloxamer 407 (molecular weight 12600, batch number WPAK592B) and poloxamer 188 (molecular weight 8400, batch number WPAK539B) were purchased from Badische Anilin-und-Soda-Fabrik (BASF; Ludwigshafen, Germany).

Techniques: Infection, Software