Journal: Experimental and Therapeutic Medicine

Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

doi: 10.3892/etm.2021.10637

Figure Lengend Snippet: Podofilox inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.

Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

Techniques: Incubation

Journal: Experimental and Therapeutic Medicine

Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

doi: 10.3892/etm.2021.10637

Figure Lengend Snippet: Podofilox inhibits cell colony formation. (A) AGS and (B) HGC-27 cells were incubated with vehicle or 3.4 nM podofilox for 7 days, and cell colony formation was assessed. ** P<0.01 and *** P<0.001 vs. vehicle.

Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

Techniques: Incubation

Journal: Experimental and Therapeutic Medicine

Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

doi: 10.3892/etm.2021.10637

Figure Lengend Snippet: Podofilox inhibits cell cycle progression of HGC-27 and AGS cells. (A and B) The cell cycle was analyzed in (A) AGS and (B) HGC-27 cells following incubation with vehicle or 3.4 nM podofilox for 48 h. Left, representative images; right, quantification analysis. * P<0.05 and ** P<0.01 vs. vehicle.

Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

Techniques: Incubation

Journal: Experimental and Therapeutic Medicine

Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

doi: 10.3892/etm.2021.10637

Figure Lengend Snippet: Microarray analysis of dysregulated genes following treatment with podofilox. (A) Heatmap of dysregulated genes in AGS cells treated with vehicle or podofilox. A total of 566 genes were upregulated, and 713 genes were downregulated. Green, downregulated genes; red, upregulated genes. (B) KEGG and HALLMARK pathway enrichment analyses of cell cycle, c-Myc and p53 signaling pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

Techniques: Microarray, Protein-Protein interactions

Journal: Experimental and Therapeutic Medicine

Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

doi: 10.3892/etm.2021.10637

Figure Lengend Snippet: Western blot analysis of dysregulated genes following treatment with podofilox. Immunoblots of HK2, PKM2, p53, c-Myc, ATG10 and GAPDH following incubation with vehicle or 3.4 nM podofilox for 48 h are presented on the left, and the quantitative analysis is presented on the right. * P<0.05 and ** P<0.01 vs. vehicle. HK2, hexokinase 2; PKM2, pyruvate kinase M1/2; ATG10, autophagy-related 10.

Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

Techniques: Western Blot, Incubation