Journal: Experimental and Therapeutic Medicine

Article Title: LINK-A lncRNA activates HIF1α signaling and inhibits podocyte cell apoptosis in diabetic nephropathy

doi: 10.3892/etm.2019.7542

Figure Lengend Snippet: LINK-A lncRNA and HIF1α overexpression inhibited the apoptosis of mouse podocyte cells under a high glucose treatment. Data of the cell apoptosis assay revealed that (A) LINK-A lncRNA and (B) HIF1α overexpression inhibited apoptosis of mouse podocyte cells under a high glucose treatment. *P<0.05. lncRNA, long non-coding RNA; HIF1α, hypoxia-inducible factor 1α; C, Control; NC, negative control; LINK-A, long intergenic non-coding RNA for kinase activation.

Article Snippet: Cells were cultured in mouse podocyte primary cell culture complete medium with serum (Celprogen, Inc.) and maintained at 37°C in a 5% CO 2 -humidifed incubator.

Techniques: Over Expression, Apoptosis Assay, Negative Control, Activation Assay

Journal: Experimental and Therapeutic Medicine

Article Title: LINK-A lncRNA activates HIF1α signaling and inhibits podocyte cell apoptosis in diabetic nephropathy

doi: 10.3892/etm.2019.7542

Figure Lengend Snippet: LINK-A lncRNA activates HIF1α in mouse podocyte cells. The effect of LINK-A lncRNA overexpression on (A) HIF1α expression and HIF1α overexpression on (B) LINK-A lncRNA expression in mouse podocyte cells was assessed. *P<0.05. lncRNA, long non-coding RNA; HIF1α, hypoxia-inducible factor 1α; C, Control; NC, negative control; LINK-A, long intergenic non-coding RNA for kinase activation.

Article Snippet: Cells were cultured in mouse podocyte primary cell culture complete medium with serum (Celprogen, Inc.) and maintained at 37°C in a 5% CO 2 -humidifed incubator.

Techniques: Over Expression, Expressing, Negative Control, Activation Assay

Journal: Pflugers Archiv : European journal of physiology

Article Title: Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes

doi: 10.1007/s00424-022-02767-8

Figure Lengend Snippet: A. Representative on-cell current traces measured at −Vp = +50 mV in podocytes previously treated 24 h with vehicle (upper trace) or with IFNγ (10 ng/mL, lower trace). B. I-V curve of the predominant unitary conductance class (28 pS) of on-cell patch currents in a representative IFNγ-treated Celprogen human podocyte. C. NPo of predominant unitary current class in native Celprogen podocytes (WT, middle pair of bars) and in two Celprogen knockout cell lines (KO #1 and KO #2), each treated 24 h in the absence (left unfilled bars of each pair) and presence of IFNγ (right bars of each pair; data points overlap in all bars). Values are means ± s.e.m. for indicated (n). *, p<0.05 for WT+IFNγ vs −IFNγ, and for WT+IFNγ vs each KO+IFNγ; unpaired two-way t-tests.

Article Snippet: On-cell patch recording revealed G2 knock-in mouse podocyte cation currents with unitary conductance of 25.2±2.5 pS and NPo of 1.41±0.24 (n=3, −Vp = +50 mV, data not shown), similar to values recorded in on-cell patches of IFNγ-treated Celprogen human G0 podocytes ( ).

Techniques: Knock-Out

Journal: Pflugers Archiv : European journal of physiology

Article Title: Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes

doi: 10.1007/s00424-022-02767-8

Figure Lengend Snippet: A. NPo of unitary currents measured at −Vp = +50 mV in IFNγ-pretreated Celprogen podocytes treated without (n=14, white bar, data points overlap) or with 2 μg/ml anti-APOL1 N-terminal domain antibody (n=7, black bar, data points overlap) or nonspecific rabbit IgG (n=6, gray bar) in the pipette solution. Steady-state values at 2–3 min after achievement of seal. *, p=0.003 vs “none”; unpaired two-tailed t-test. B. NPo of unitary currents measured at −Vp = +50 mV in IFNγ-pretreated Celprogen podocytes (n=6) exposed to 3 μM recombinant N-terminal fragment of Serum Response-Associated (SRA) of Trypanosoma brucei rhodesiense in the pipette solution, and recorded immediately after establishment of a gigohm seal (gray bar) and ~110 s after seal establishment (black bar; data points overlap). *, p<0.041 vs ~0 sec; unpaired two-tailed t-test. Gigohm resistances were maintained throughout the recording periods.

Article Snippet: On-cell patch recording revealed G2 knock-in mouse podocyte cation currents with unitary conductance of 25.2±2.5 pS and NPo of 1.41±0.24 (n=3, −Vp = +50 mV, data not shown), similar to values recorded in on-cell patches of IFNγ-treated Celprogen human G0 podocytes ( ).

Techniques: Transferring, Two Tailed Test, Recombinant