Journal: Cell reports

Article Title: Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration

doi: 10.1016/j.celrep.2022.110473

Figure Lengend Snippet: KEY RESOURCES TABLE Primary and secondary antibodies

Article Snippet: Anti-Podocin , Novus Biologicals , JB51–33 , NBP2–75624.

Techniques:

Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: Effect of Nephropathy Prescription I on the Expression of Angptl3 and Podocyte-Associated Protein in Mice with Adriamycin-Induced Nephropathy.

doi: 10.1155/2022/9921679

Figure Lengend Snippet: Figure 8: Protein levels of nephrin, podocin, and Angptl3 in different groups: (a) control (C); (b) model group (M); (c) traditional Chinese medicine (TCM) group (M + Z); (d) (M + S); and (e) (M + Z + S). ∗M vs. C group (P < 0.05); &M + Z or M + S vs. M group (P < 0.05); #M + Z + S vs. M group (P < 0.01).

Article Snippet: Adriamycin (D1515, 10mg) was purchased from Sigma; prednisone (5mg) was obtained from Sinepharm (H31020675, Shanghai, China); antibodies against nephrin (Novus, NBP1-30130), podocin (Novus, NBP226057), and angptl3 (RnD, AF136) were used.

Techniques: Control

Journal: The Journal of Clinical Investigation

Article Title: CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease

doi: 10.1172/JCI99507

Figure Lengend Snippet: MRL.lpr mice were injected i.p. with free KN93 (10 μg/wk), anti-podocin or anti-nephrin antibody–coated KN93-loaded nlg (10 μg of KN93/week), or empty nlg at from 8 to 16 weeks of age (n = 5–7 mice in each group). (A) Urine albumin/creatinine (Alb/Cre) ratio. Urine samples were obtained biweekly, and albumin and creatinine levels were determined by ELISA. Error bars represent mean ± SEM. *P < 0.05, 2-way ANOVA with Bonferroni’s post test. (B and C) Representative images of glomeruli from 16-week- old MRL.lpr mice treated with anti-nephrin antibody–coated empty nlg or KN93-loaded nlg. PAS (B) and Masson’s trichrome staining (C) are shown. The arrows point to the crescent. Scale bars: 50 μm. (D) Mean histological scores in the kidneys of mice from the indicated treatment groups (n = 5 in each group). *P < 0.05; **P < 0.01, Student’s t test. (E and F) Electron microscopic images of a glomerulus from a mouse treated with anti-nephrin empty nlg (left) showing diffuse podocyte foot process (FP) effacement with slit diaphragm occlusion (arrows) and subepithelial dense deposits (asterisks) and a mouse treated with anti-nephrin KN93-loaded nlg (right), which shows podocytes with normal foot processes and slit diaphragms and no deposits in the basement membrane. Original magnification, ×8,000 (E); ×30,000 (F). Three glomeruli were evaluated in each of 3 mice in each experimental condition. (G) C3 and IgG deposition was significantly suppressed in the glomeruli of MRL.lpr mice treated with KN93 targeted to podocytes, as indicated. Kidney sections from MRL/MpJ (16 weeks of age) mice are shown as controls (n = 5 mice in each group). (H) Nephrin (green) and synaptopodin (red) expression detected by immunofluorescence. Scale bars: 50 μm. (I) The fluorescence intensity of nephrin and synaptopodin quantified in glomeruli from mice subjected to the indicated treatments. ****P < 0.0001, Student’s t test. (J) Nphs1, Nphs2, and Synpo expression in glomeruli from mice treated with anti-nephrin–coated empty or KN93-loaded nlg. Results were normalized to the expression of GAPDH (n = 5 in each group). ****P < 0.0001, Student’s t test.

Article Snippet: Isolated cells were stained for flow cytometry with antibodies against nephrin (bs-10233R-A488, Bioss Antibodies) and podocin (bs-6597R-A647, Bioss Antibodies).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Immunofluorescence, Fluorescence

Journal: The Journal of Clinical Investigation

Article Title: CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease

doi: 10.1172/JCI99507

Figure Lengend Snippet: MRL.lpr mice were injected i.p. with free KN93 (10 μg/wk), anti-podocin or anti-nephrin antibody–coated KN93-loaded nlg (10 μg of KN93/week), or empty nlg at from 8 to 16 weeks of age (n = 5–7 mice in each group). (A) Urine albumin/creatinine (Alb/Cre) ratio. Urine samples were obtained biweekly, and albumin and creatinine levels were determined by ELISA. Error bars represent mean ± SEM. *P < 0.05, 2-way ANOVA with Bonferroni’s post test. (B and C) Representative images of glomeruli from 16-week- old MRL.lpr mice treated with anti-nephrin antibody–coated empty nlg or KN93-loaded nlg. PAS (B) and Masson’s trichrome staining (C) are shown. The arrows point to the crescent. Scale bars: 50 μm. (D) Mean histological scores in the kidneys of mice from the indicated treatment groups (n = 5 in each group). *P < 0.05; **P < 0.01, Student’s t test. (E and F) Electron microscopic images of a glomerulus from a mouse treated with anti-nephrin empty nlg (left) showing diffuse podocyte foot process (FP) effacement with slit diaphragm occlusion (arrows) and subepithelial dense deposits (asterisks) and a mouse treated with anti-nephrin KN93-loaded nlg (right), which shows podocytes with normal foot processes and slit diaphragms and no deposits in the basement membrane. Original magnification, ×8,000 (E); ×30,000 (F). Three glomeruli were evaluated in each of 3 mice in each experimental condition. (G) C3 and IgG deposition was significantly suppressed in the glomeruli of MRL.lpr mice treated with KN93 targeted to podocytes, as indicated. Kidney sections from MRL/MpJ (16 weeks of age) mice are shown as controls (n = 5 mice in each group). (H) Nephrin (green) and synaptopodin (red) expression detected by immunofluorescence. Scale bars: 50 μm. (I) The fluorescence intensity of nephrin and synaptopodin quantified in glomeruli from mice subjected to the indicated treatments. ****P < 0.0001, Student’s t test. (J) Nphs1, Nphs2, and Synpo expression in glomeruli from mice treated with anti-nephrin–coated empty or KN93-loaded nlg. Results were normalized to the expression of GAPDH (n = 5 in each group). ****P < 0.0001, Student’s t test.

Article Snippet: To achieve targeting ability for podocytes, nlg was incubated with biotin-conjugated anti-mouse podocin (bs-6597R-Biotin, Bioss Antibodies) or nephrin antibodies (bs-10233R-Biotin, Bioss Antibodies) for 20 minutes at room temperature before injection.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Immunofluorescence, Fluorescence