pnl4 3 Search Results


page e1 cell reports 6  (Addgene inc)
92
Addgene inc page e1 cell reports 6
Page E1 Cell Reports 6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
page e1 cell reports 6 - by Bioz Stars, 2026-04
92/100 stars
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hiv 1  (Addgene inc)
88
Addgene inc hiv 1
Hiv 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiv 1/product/Addgene inc
Average 88 stars, based on 1 article reviews
hiv 1 - by Bioz Stars, 2026-04
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pnl4 3 env  (Addgene inc)
93
Addgene inc pnl4 3 env
Pnl4 3 Env, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pnl4 3 env - by Bioz Stars, 2026-04
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pnl4 3  (Addgene inc)
93
Addgene inc pnl4 3
Pnl4 3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pnl4 3 - by Bioz Stars, 2026-04
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pnl4-3-gfp  (Promega)
90
Promega pnl4-3-gfp
Pnl4 3 Gfp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pnl4-3-gfp - by Bioz Stars, 2026-04
90/100 stars
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pnl4.3 plasmid  (Inserm Transfert)
90
Inserm Transfert pnl4.3 plasmid
Pnl4.3 Plasmid, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pnl4.3 plasmid - by Bioz Stars, 2026-04
90/100 stars
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pnl4.3 plasmid  (BEI Resources)
90
BEI Resources pnl4.3 plasmid
Pnl4.3 Plasmid, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pnl4.3 plasmid - by Bioz Stars, 2026-04
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pnl4-3 plasmid dna  (SignaGen)
90
SignaGen pnl4-3 plasmid dna
(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT <t>pNL4.3.</t> Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Pnl4 3 Plasmid Dna, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnl4-3 plasmid dna/product/SignaGen
Average 90 stars, based on 1 article reviews
pnl4-3 plasmid dna - by Bioz Stars, 2026-04
90/100 stars
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full-length pnl4-3 derivatives  (CEM Corporation)
90
CEM Corporation full-length pnl4-3 derivatives
(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT <t>pNL4.3.</t> Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Full Length Pnl4 3 Derivatives, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/full-length pnl4-3 derivatives/product/CEM Corporation
Average 90 stars, based on 1 article reviews
full-length pnl4-3 derivatives - by Bioz Stars, 2026-04
90/100 stars
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pnl4-3 rev mason-pfizer monkey virus cte  (Pfizer Inc)
90
Pfizer Inc pnl4-3 rev mason-pfizer monkey virus cte
(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT <t>pNL4.3.</t> Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Pnl4 3 Rev Mason Pfizer Monkey Virus Cte, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnl4-3 rev mason-pfizer monkey virus cte/product/Pfizer Inc
Average 90 stars, based on 1 article reviews
pnl4-3 rev mason-pfizer monkey virus cte - by Bioz Stars, 2026-04
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hiv-1 infectious pnl4-3 plasmid clone  (Pasteur Institute)
90
Pasteur Institute hiv-1 infectious pnl4-3 plasmid clone
(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT <t>pNL4.3.</t> Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Hiv 1 Infectious Pnl4 3 Plasmid Clone, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hiv-1 infectious pnl4-3 plasmid clone - by Bioz Stars, 2026-04
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pnl4.3-rfp pseudovirus  (PSICOR Inc)
90
PSICOR Inc pnl4.3-rfp pseudovirus
(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT <t>pNL4.3.</t> Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Pnl4.3 Rfp Pseudovirus, supplied by PSICOR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT pNL4.3. Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.

Journal: bioRxiv

Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir

doi: 10.1101/2024.12.06.627250

Figure Lengend Snippet: (A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT pNL4.3. Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.

Article Snippet: Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 µg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol.

Techniques: Produced, Control, Isolation, Infection, Luciferase, Activity Assay

(A) The experimental design. The same virus preparation was used for three different assays: 1) LC-MS/MS to determine LEN amounts bound to virions, 2) p24 ELISA to determine CA amounts; and 3) infectivity of the viruses produced in the presence of LEN or DMSO control in HeLa TZM-bl cells. (B) Representative LC-MS/MS results of viruses produced in the presence of LEN (the top panel), the identical concentration of LEN added to HEK293T cells in the absence of pNL4.3 (the middle panel), and the control of the medium without LEN or the virus (the bottom panel). (C) Infectivity of the viruses at indicated [LEN]:[CA] ratios or DMSO control. The [LEN]:[CA] ratios were measured using p24 ELISA and LC-MS/MS results for the same virus preparations.

Journal: bioRxiv

Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir

doi: 10.1101/2024.12.06.627250

Figure Lengend Snippet: (A) The experimental design. The same virus preparation was used for three different assays: 1) LC-MS/MS to determine LEN amounts bound to virions, 2) p24 ELISA to determine CA amounts; and 3) infectivity of the viruses produced in the presence of LEN or DMSO control in HeLa TZM-bl cells. (B) Representative LC-MS/MS results of viruses produced in the presence of LEN (the top panel), the identical concentration of LEN added to HEK293T cells in the absence of pNL4.3 (the middle panel), and the control of the medium without LEN or the virus (the bottom panel). (C) Infectivity of the viruses at indicated [LEN]:[CA] ratios or DMSO control. The [LEN]:[CA] ratios were measured using p24 ELISA and LC-MS/MS results for the same virus preparations.

Article Snippet: Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 µg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol.

Techniques: Virus, Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Infection, Produced, Control, Concentration Assay