pna-lna clamp method Search Results


peptide nucleic acid-locked clamping method  (Nagai Nori USA INC)
90
Nagai Nori USA INC peptide nucleic acid-locked clamping method
Peptide Nucleic Acid Locked Clamping Method, supplied by Nagai Nori USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pna-lna+clamp+method/peptide+nucleic+acid+locked+nucleic+acid++pna+lna++polymerase+chain+reaction++pcr++clamp+method/pm19680292-109-25-30
Average 90 stars, based on 1 article reviews
peptide nucleic acid-locked clamping method - by Bioz Stars, 2026-10
90/100 stars
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pna-lna pcr clamp method  (srl inc)
90
srl inc pna-lna pcr clamp method
(a). Specimen collection. Among lung carcinoma patients treated at the University of Tsukuba Hospital (Ibaraki, Japan) between 2015 and 2019, sBWF samples were obtained from 1159, and EGFR mutation testing <t>(PNA-LNA</t> <t>PCR</t> Clamp Method or Cobas EGFR Mutation Test v2) was undertaken for 310 using tissue samples (biopsy or surgical specimens). From among these cases, 80 were selected. Among them, the ex21 L858R EGFR point mutation was detected in 26 cases and the ex19 E746-A750 deletion mutation in 24; EGFR mutations were undetectable in 30 cases. Blood serum samples were included in three, three, and two cases, respectively. (b) Comparison of EGFR genotyping using cfDNA from supernatant of bronchial washing fluid (sBWF) with that using tissue samples (biopsy or surgical specimens). The positivity rate for EGFR mutation using cfDNA extracted from sBWF was 80% (40/50, ex21 L858R in 20/26 cases and ex19 E746-A750 del in 20/24 cases). Thirty cases were negative for EGFR mutation, but one case showed >2 copies of EGFR mutation/μL (ex21 L858R).
Pna Lna Pcr Clamp Method, supplied by srl inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pna-lna+clamp+method/pna+lna+pcr+clamp+method/pmc11144292-71-18-28
Average 90 stars, based on 1 article reviews
pna-lna pcr clamp method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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(a). Specimen collection. Among lung carcinoma patients treated at the University of Tsukuba Hospital (Ibaraki, Japan) between 2015 and 2019, sBWF samples were obtained from 1159, and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) was undertaken for 310 using tissue samples (biopsy or surgical specimens). From among these cases, 80 were selected. Among them, the ex21 L858R EGFR point mutation was detected in 26 cases and the ex19 E746-A750 deletion mutation in 24; EGFR mutations were undetectable in 30 cases. Blood serum samples were included in three, three, and two cases, respectively. (b) Comparison of EGFR genotyping using cfDNA from supernatant of bronchial washing fluid (sBWF) with that using tissue samples (biopsy or surgical specimens). The positivity rate for EGFR mutation using cfDNA extracted from sBWF was 80% (40/50, ex21 L858R in 20/26 cases and ex19 E746-A750 del in 20/24 cases). Thirty cases were negative for EGFR mutation, but one case showed >2 copies of EGFR mutation/μL (ex21 L858R).

Journal: Japanese Journal of Clinical Oncology

Article Title: High-efficiency EGFR genotyping using cell-free DNA in bronchial washing fluid

doi: 10.1093/jjco/hyae021

Figure Lengend Snippet: (a). Specimen collection. Among lung carcinoma patients treated at the University of Tsukuba Hospital (Ibaraki, Japan) between 2015 and 2019, sBWF samples were obtained from 1159, and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) was undertaken for 310 using tissue samples (biopsy or surgical specimens). From among these cases, 80 were selected. Among them, the ex21 L858R EGFR point mutation was detected in 26 cases and the ex19 E746-A750 deletion mutation in 24; EGFR mutations were undetectable in 30 cases. Blood serum samples were included in three, three, and two cases, respectively. (b) Comparison of EGFR genotyping using cfDNA from supernatant of bronchial washing fluid (sBWF) with that using tissue samples (biopsy or surgical specimens). The positivity rate for EGFR mutation using cfDNA extracted from sBWF was 80% (40/50, ex21 L858R in 20/26 cases and ex19 E746-A750 del in 20/24 cases). Thirty cases were negative for EGFR mutation, but one case showed >2 copies of EGFR mutation/μL (ex21 L858R).

Article Snippet: Tissue samples were fixed with 10% neutral buffered formalin and used for histological diagnosis and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) (SRL Inc, Tokyo, Japan).

Techniques: Mutagenesis, Comparison

Comparison of various EGFR analysis methods using BWF

Journal: Japanese Journal of Clinical Oncology

Article Title: High-efficiency EGFR genotyping using cell-free DNA in bronchial washing fluid

doi: 10.1093/jjco/hyae021

Figure Lengend Snippet: Comparison of various EGFR analysis methods using BWF

Article Snippet: Tissue samples were fixed with 10% neutral buffered formalin and used for histological diagnosis and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) (SRL Inc, Tokyo, Japan).

Techniques: Comparison, Real-time Polymerase Chain Reaction