Journal: STAR Protocols

Article Title: Fluorogenic in vitro activity assay for the main protease M pro from SARS-CoV-2 and its adaptation to the identification of inhibitors

doi: 10.1016/j.xpro.2021.100793

Figure Lengend Snippet: Required Equipment Image of the SpectraMax M5 plate reader (left) and photos taken during the activity measurement (right), i.e., multi-channel pipette, black multiwell plate, vial with protease stock solution, polypropylene tube for preparation of working solution and assay buffer (in the reservoir). The use of pipette tips with filter is facultative.

Article Snippet: Timing: 15 min Use a plate reader with fluorescence function, e.g., SpectraMax M5 plate reader (black multiwell plate, PMT setting “low”, 0.2 mL assay volume).

Techniques: Activity Assay, Transferring

Journal: STAR Protocols

Article Title: Fluorogenic in vitro activity assay for the main protease M pro from SARS-CoV-2 and its adaptation to the identification of inhibitors

doi: 10.1016/j.xpro.2021.100793

Figure Lengend Snippet: Example of standard curve of the fluorescent moiety AMC alone in assay buffer and data measured with the SpectraMax M5 plate reader (black multiwell plate, PMT setting “low”, 0.2 mL assay volume) This result is device-dependent.

Article Snippet: Timing: 15 min Use a plate reader with fluorescence function, e.g., SpectraMax M5 plate reader (black multiwell plate, PMT setting “low”, 0.2 mL assay volume).

Techniques:

Journal: STAR Protocols

Article Title: Fluorogenic in vitro activity assay for the main protease M pro from SARS-CoV-2 and its adaptation to the identification of inhibitors

doi: 10.1016/j.xpro.2021.100793

Figure Lengend Snippet:

Article Snippet: Timing: 15 min Use a plate reader with fluorescence function, e.g., SpectraMax M5 plate reader (black multiwell plate, PMT setting “low”, 0.2 mL assay volume).

Techniques: Recombinant