pmca4 specific shrna plasmid Search Results


shrna expressing mcf 7 cells  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology shrna expressing mcf 7 cells
Shrna Expressing Mcf 7 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmca4+specific+shrna+plasmid/PMCA4+shRNA+Plasmid/pm40075218-250-6-19
Average 92 stars, based on 1 article reviews
shrna expressing mcf 7 cells - by Bioz Stars, 2026-09
92/100 stars
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mutant pmca4  (Thermo Fisher)
99
Thermo Fisher mutant pmca4
(A) Western blot showing stable overexpression of either wild-type (WT) or R268Q mutant <t>ATP2B4</t> protein at similar level in SH-SY5Y cells. (B) ATP2B4 mRNA levels in WT and mutant stably overexpressing cells were similar as shown by quantitative real-time PCR. ** represents statistical significance at p <0.01 as compared to vector. ns: Not statistically significant.
Mutant Pmca4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmca4+specific+shrna+plasmid/FURA+2-AM/pmc04356846-46-18-30
Average 99 stars, based on 1 article reviews
mutant pmca4 - by Bioz Stars, 2026-09
99/100 stars
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pmca4 plasmid  (Addgene inc)
92
Addgene inc pmca4 plasmid
(A) Western blot showing stable overexpression of either wild-type (WT) or R268Q mutant <t>ATP2B4</t> protein at similar level in SH-SY5Y cells. (B) ATP2B4 mRNA levels in WT and mutant stably overexpressing cells were similar as shown by quantitative real-time PCR. ** represents statistical significance at p <0.01 as compared to vector. ns: Not statistically significant.
Pmca4 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmca4+specific+shrna+plasmid/pcDNA3%2E1-hPMCA4b+(Plasmid+%2347595)/pm32860837-46-57-59
Average 92 stars, based on 1 article reviews
pmca4 plasmid - by Bioz Stars, 2026-09
92/100 stars
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pcdna3.1-hpmca4b-d672e (plasmid #47596)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pcdna3.1-mpmca4b (plasmid #47597)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pmca4 lentiviral activation particles  (Santa Cruz Biotechnology)
N/A
CRISPR/Cas9 KO Plasmids consists of PMCA4-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
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pmca4 shrna (h) lentiviral particles  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of PMCA4 gene silencing results, individual duplex components or plasmids are also available upon request. Suitable
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egfp-hpmca4b delta6 (plasmid #47590)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pmca4 sirna  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of PMCA4 gene silencing results, individual duplex components or plasmids are also available upon request. Suitable
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egfp-hpmca4bct103 (plasmid #47592)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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egfp-hpmca4b (plasmid #47589)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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egfp-hpmca4bct125 (plasmid #47594)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Western blot showing stable overexpression of either wild-type (WT) or R268Q mutant ATP2B4 protein at similar level in SH-SY5Y cells. (B) ATP2B4 mRNA levels in WT and mutant stably overexpressing cells were similar as shown by quantitative real-time PCR. ** represents statistical significance at p <0.01 as compared to vector. ns: Not statistically significant.

Journal: Brain and Behavior

Article Title: PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion

doi: 10.1002/brb3.321

Figure Lengend Snippet: (A) Western blot showing stable overexpression of either wild-type (WT) or R268Q mutant ATP2B4 protein at similar level in SH-SY5Y cells. (B) ATP2B4 mRNA levels in WT and mutant stably overexpressing cells were similar as shown by quantitative real-time PCR. ** represents statistical significance at p <0.01 as compared to vector. ns: Not statistically significant.

Article Snippet: To determine the effects of PMCA4 mutation on the intracellular calcium transient, cells stably overexpressing either wild-type or mutant PMCA4 were labeled with 5 μ mol/L fura2 -AM fluorescent dye (Invitrogen) in serum-free DMEM for 20 min in darkness at room temperature.

Techniques: Western Blot, Over Expression, Mutagenesis, Stable Transfection, Real-time Polymerase Chain Reaction, Plasmid Preparation

(A) Real-time plot of changes in cytosolic calcium level in SH-SY5Y cells overexpressing either WT or R268Q mutant ATP2B4 by KCl-induced depolarization. Cells were labeled with 5 μ mol/L fura2-AM fluorescent dye in serum-free DMEM for 20 min at room temperature. Relative changes in intracellular calcium levels [Ca 2+ ] i , indicated as normalized OD 340/380 ratio were plotted against time. Dotted lines represent 95% confident intervals (CI) of the corresponding curves. (B) Maximum cytosolic calcium surge indicated as KCl-induced peak [Ca 2+ ] i of individual cells within the monitored period are shown in bar chart. N = Number of individual cells measured in each group. Data represent mean ± SD. Comparison between groups was performed using unpaired t -test. R268Q mutant ATP2B4 significantly delayed attenuation of KCl-induced calcium surge compared to wild-type. **represents statistical significance as compared with vector controls at P < 0.01; ## represents P < 0.01 between the two designated groups.

Journal: Brain and Behavior

Article Title: PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion

doi: 10.1002/brb3.321

Figure Lengend Snippet: (A) Real-time plot of changes in cytosolic calcium level in SH-SY5Y cells overexpressing either WT or R268Q mutant ATP2B4 by KCl-induced depolarization. Cells were labeled with 5 μ mol/L fura2-AM fluorescent dye in serum-free DMEM for 20 min at room temperature. Relative changes in intracellular calcium levels [Ca 2+ ] i , indicated as normalized OD 340/380 ratio were plotted against time. Dotted lines represent 95% confident intervals (CI) of the corresponding curves. (B) Maximum cytosolic calcium surge indicated as KCl-induced peak [Ca 2+ ] i of individual cells within the monitored period are shown in bar chart. N = Number of individual cells measured in each group. Data represent mean ± SD. Comparison between groups was performed using unpaired t -test. R268Q mutant ATP2B4 significantly delayed attenuation of KCl-induced calcium surge compared to wild-type. **represents statistical significance as compared with vector controls at P < 0.01; ## represents P < 0.01 between the two designated groups.

Article Snippet: To determine the effects of PMCA4 mutation on the intracellular calcium transient, cells stably overexpressing either wild-type or mutant PMCA4 were labeled with 5 μ mol/L fura2 -AM fluorescent dye (Invitrogen) in serum-free DMEM for 20 min in darkness at room temperature.

Techniques: Mutagenesis, Labeling, Comparison, Plasmid Preparation

(A) Real-time plot of changes in cytosolic calcium level in SH-SY5Y cells overexpressing either WT or R268Q mutant ATP2B4 by SERCA inhibition using thapsigargin. Cells were labeled with 5 μ mo/L fura2-AM fluorescent dye in serum-free DMEM for 20 min at room temperature. Relative changes in intracellular calcium levels [Ca 2+ ] i , indicated as normalized OD 340/380 ratio were plotted against time. (B) The mean of maximum steady level of cytosolic calcium of individual cells within the monitored period are shown in bar chart. N = Number of individual cells measured in each group. Data represent mean ± SD. Comparison between groups was performed using unpaired t-test. Cells overexpressing R268Q mutant protein demonstrated significantly higher cytosolic calcium concentration compared to wild-type. *represents statistical significance as compared with WT at P < 0.05.

Journal: Brain and Behavior

Article Title: PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion

doi: 10.1002/brb3.321

Figure Lengend Snippet: (A) Real-time plot of changes in cytosolic calcium level in SH-SY5Y cells overexpressing either WT or R268Q mutant ATP2B4 by SERCA inhibition using thapsigargin. Cells were labeled with 5 μ mo/L fura2-AM fluorescent dye in serum-free DMEM for 20 min at room temperature. Relative changes in intracellular calcium levels [Ca 2+ ] i , indicated as normalized OD 340/380 ratio were plotted against time. (B) The mean of maximum steady level of cytosolic calcium of individual cells within the monitored period are shown in bar chart. N = Number of individual cells measured in each group. Data represent mean ± SD. Comparison between groups was performed using unpaired t-test. Cells overexpressing R268Q mutant protein demonstrated significantly higher cytosolic calcium concentration compared to wild-type. *represents statistical significance as compared with WT at P < 0.05.

Article Snippet: To determine the effects of PMCA4 mutation on the intracellular calcium transient, cells stably overexpressing either wild-type or mutant PMCA4 were labeled with 5 μ mol/L fura2 -AM fluorescent dye (Invitrogen) in serum-free DMEM for 20 min in darkness at room temperature.

Techniques: Mutagenesis, Inhibition, Labeling, Comparison, Concentration Assay