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Amaxa shirf-4 plus shirf-5 (shirf-52 + shirf-54; open circle )
IRF-4 negatively regulates IRF-5 expression. IB4 cells were transfected with shLuc (5 μg), <t>shIRF-4</t> (shIRF-41/shIRF-42/shIRF-43, 1:1:1 ratio, 5 μg), pcDNA3 (5 μg), or IRF-4 expression plasmid (1 μg) by use of a nucleofector device from Amaxa. Total DNA were normalized to 5 μg with vector DNA. Cells were collected 3 days later. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots. The identities of the proteins are shown. The images in the same box indicate that they are derived from the same membrane.
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IRF-4 negatively regulates IRF-5 expression. IB4 cells were transfected with shLuc (5 μg), <t>shIRF-4</t> (shIRF-41/shIRF-42/shIRF-43, 1:1:1 ratio, 5 μg), pcDNA3 (5 μg), or IRF-4 expression plasmid (1 μg) by use of a nucleofector device from Amaxa. Total DNA were normalized to 5 μg with vector DNA. Cells were collected 3 days later. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots. The identities of the proteins are shown. The images in the same box indicate that they are derived from the same membrane.
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IRF-4 negatively regulates IRF-5 expression. IB4 cells were transfected with shLuc (5 μg), <t>shIRF-4</t> (shIRF-41/shIRF-42/shIRF-43, 1:1:1 ratio, 5 μg), pcDNA3 (5 μg), or IRF-4 expression plasmid (1 μg) by use of a nucleofector device from Amaxa. Total DNA were normalized to 5 μg with vector DNA. Cells were collected 3 days later. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots. The identities of the proteins are shown. The images in the same box indicate that they are derived from the same membrane.
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IRF-4 negatively regulates IRF-5 expression. IB4 cells were transfected with shLuc (5 μg), shIRF-4 (shIRF-41/shIRF-42/shIRF-43, 1:1:1 ratio, 5 μg), pcDNA3 (5 μg), or IRF-4 expression plasmid (1 μg) by use of a nucleofector device from Amaxa. Total DNA were normalized to 5 μg with vector DNA. Cells were collected 3 days later. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots. The identities of the proteins are shown. The images in the same box indicate that they are derived from the same membrane.

Journal: The Journal of Biological Chemistry

Article Title: Interferon Regulatory Factor 4 (IRF-4) Targets IRF-5 to Regulate Epstein-Barr Virus Transformation *

doi: 10.1074/jbc.M110.210542

Figure Lengend Snippet: IRF-4 negatively regulates IRF-5 expression. IB4 cells were transfected with shLuc (5 μg), shIRF-4 (shIRF-41/shIRF-42/shIRF-43, 1:1:1 ratio, 5 μg), pcDNA3 (5 μg), or IRF-4 expression plasmid (1 μg) by use of a nucleofector device from Amaxa. Total DNA were normalized to 5 μg with vector DNA. Cells were collected 3 days later. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots. The identities of the proteins are shown. The images in the same box indicate that they are derived from the same membrane.

Article Snippet: A , IB4 cells were transfected with shLuc (5 μg; line +), shIRF-4 (shIRF-41 + shIRF-42 + shIRF-43, 1:1:1 ratio, 2.5 μg; line *), shIRF5 (shIRF-51 + shIRF-53, 1:1 ratio, 2.5 μg; solid square ), shIRF4 plus shIRF-5 (shIRF-51 + shIRF-53; open square ), shIRF-5 (shIRF-52 + shIRF-54, 1:1 ratio, 2.5 μg; solid circle ), or shIRF-4 plus shIRF-5 (shIRF-52 + shIRF-54; open circle ) by Amaxa nucleofector technology.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Derivative Assay

Knockdown of IRF-5 rescues IRF-4 knockdown-mediated growth inhibition. A, IB4 cells were transfected with shLuc (5 μg; line +), shIRF-4 (shIRF-41 + shIRF-42 + shIRF-43, 1:1:1 ratio, 2.5 μg; line *), shIRF5 (shIRF-51 + shIRF-53, 1:1 ratio, 2.5 μg; solid square), shIRF4 plus shIRF-5 (shIRF-51 + shIRF-53; open square), shIRF-5 (shIRF-52 + shIRF-54, 1:1 ratio, 2.5 μg; solid circle), or shIRF-4 plus shIRF-5 (shIRF-52 + shIRF-54; open circle) by Amaxa nucleofector technology. Total DNA was normalized to 5 μg with shLuc DNA. One day (D) after the transfection, live cells were isolated. At the indicated days after transfection, surviving cells were enumerated. Each point represents the number of live cells (means ± S.D.). The use of shIRF52 plus shIRF-54 also produced curative data. Results from one representative experiment are as shown. B, expression levels of the various proteins. IB4 cells were transfected with various plasmids as shown at the top. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots 2 days after transfection. The identities of the proteins are shown.

Journal: The Journal of Biological Chemistry

Article Title: Interferon Regulatory Factor 4 (IRF-4) Targets IRF-5 to Regulate Epstein-Barr Virus Transformation *

doi: 10.1074/jbc.M110.210542

Figure Lengend Snippet: Knockdown of IRF-5 rescues IRF-4 knockdown-mediated growth inhibition. A, IB4 cells were transfected with shLuc (5 μg; line +), shIRF-4 (shIRF-41 + shIRF-42 + shIRF-43, 1:1:1 ratio, 2.5 μg; line *), shIRF5 (shIRF-51 + shIRF-53, 1:1 ratio, 2.5 μg; solid square), shIRF4 plus shIRF-5 (shIRF-51 + shIRF-53; open square), shIRF-5 (shIRF-52 + shIRF-54, 1:1 ratio, 2.5 μg; solid circle), or shIRF-4 plus shIRF-5 (shIRF-52 + shIRF-54; open circle) by Amaxa nucleofector technology. Total DNA was normalized to 5 μg with shLuc DNA. One day (D) after the transfection, live cells were isolated. At the indicated days after transfection, surviving cells were enumerated. Each point represents the number of live cells (means ± S.D.). The use of shIRF52 plus shIRF-54 also produced curative data. Results from one representative experiment are as shown. B, expression levels of the various proteins. IB4 cells were transfected with various plasmids as shown at the top. The expression levels of IRF-4, IRF-5, and GAPDH were examined by Western blots 2 days after transfection. The identities of the proteins are shown.

Article Snippet: A , IB4 cells were transfected with shLuc (5 μg; line +), shIRF-4 (shIRF-41 + shIRF-42 + shIRF-43, 1:1:1 ratio, 2.5 μg; line *), shIRF5 (shIRF-51 + shIRF-53, 1:1 ratio, 2.5 μg; solid square ), shIRF4 plus shIRF-5 (shIRF-51 + shIRF-53; open square ), shIRF-5 (shIRF-52 + shIRF-54, 1:1 ratio, 2.5 μg; solid circle ), or shIRF-4 plus shIRF-5 (shIRF-52 + shIRF-54; open circle ) by Amaxa nucleofector technology.

Techniques: Inhibition, Transfection, Isolation, Produced, Expressing, Western Blot