Journal: Phytochemistry

Article Title: Natural biflavones are potent inhibitors against SARS-CoV-2 papain-like protease

doi: 10.1016/j.phytochem.2021.112984

Figure Lengend Snippet: Functions of SARS-CoV-2 PL pro and benefits by targeting PL pro . (A) PL pro proteolyzes viral polyprotein at the LXGG site to generate mature nsp1, nsp2 and nsp3. (B) PL pro removes ISG15 and ubiquitin modifiers from host proteins to evade innate antiviral immunity.

Article Snippet: Recombinant SARS-CoV-2 papain-like protease (Glu1564-Val1880, Cat. 40593-V07E) and recombinant human pro-ISG15 protein (Met1-Ser165, Cat. 12729-HNAE) were purchased from Sino-Biological Inc (Beijing, China).

Techniques:

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: Coronavirus , SARS , SARS , PLpro , AAX16193.1 , E. coli , Sino Biological , N-(AA1541-1859)-His-C , 40524-V08E.

Techniques: Microarray, Expressing, Construct

Journal: Slas Discovery

Article Title: High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2

doi: 10.1177/2472555220963667

Figure Lengend Snippet: Papain-like protease (PLpro) ReFRAME primary high-throughput screening (HTS) assay results. Graphed is a single-point scatterplot of all 13,104 compounds tested. Each dot graphed represents the activity result of a well containing test compound (black dots) or controls (red, violet, and green dots). The gap in the sample index (well number) is due to test wells that weren’t inclusive of compounds, and hence no data are plotted and available.

Article Snippet: Based on the protein structure of PLpro, TargetMol selected 474 top-ranked docked molecules into the PLpro-targeted compound library (CADD) by molecular docking and virtually screening against 15,376 compound structures.

Techniques: High Throughput Screening Assay, HTS Assay, Activity Assay

Journal: Slas Discovery

Article Title: High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2

doi: 10.1177/2472555220963667

Figure Lengend Snippet: Confirmation of ReFRAME hit compounds in luciferase assay. Luciferase-based protease assays were performed in 96-well plates, as described in . Cells were transfected in 6-well plates and then reseeded into 96-well plates containing compounds at 20,000 cells per well for 4 h post transfection. ( A ) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) papain-like protease (PLpro) and SARS-CoV-1 PLpro activity in the presence of compounds at threefold dilutions starting at a maximum concentration of 20 µM. ( B ) Activity of indicated proteases represented as relative to protease without compound treatment. Compounds were added at threefold dilutions starting at 10 µM.

Article Snippet: Based on the protein structure of PLpro, TargetMol selected 474 top-ranked docked molecules into the PLpro-targeted compound library (CADD) by molecular docking and virtually screening against 15,376 compound structures.

Techniques: Luciferase, Transfection, Activity Assay, Concentration Assay