pll-g-peg Search Results


pll20k-g35-peg2k  (JenKem Inc)
90
JenKem Inc pll20k-g35-peg2k
Reagents and tools table
Pll20k G35 Peg2k, supplied by JenKem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg+pll+20k+g35+peg2k/pmc11696905-44-5-2
Average 90 stars, based on 1 article reviews
pll20k-g35-peg2k - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pll-peg  (JenKem Inc)
90
JenKem Inc pll-peg
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll Peg, supplied by JenKem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg/bio_rxiv__302190-102-15-16
Average 90 stars, based on 1 article reviews
pll-peg - by Bioz Stars, 2026-10
90/100 stars
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pll-g-peg  (Susos AG)
90
Susos AG pll-g-peg
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll G Peg, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg/pmc04038945-296-20-24
Average 90 stars, based on 1 article reviews
pll-g-peg - by Bioz Stars, 2026-10
90/100 stars
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poly-l-lysine-polyethylene glycol (pll-g-peg  (Susos AG)
90
Susos AG poly-l-lysine-polyethylene glycol (pll-g-peg
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Poly L Lysine Polyethylene Glycol (Pll G Peg, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/poly+l+lysine++graft+poly+ethylene+glycol++pll+g+peg/pmc03145729-230-21-24
Average 90 stars, based on 1 article reviews
poly-l-lysine-polyethylene glycol (pll-g-peg - by Bioz Stars, 2026-10
90/100 stars
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pll-g-peg solution  (Susos AG)
90
Susos AG pll-g-peg solution
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll G Peg Solution, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg+solution/pm32553185-215-11-16
Average 90 stars, based on 1 article reviews
pll-g-peg solution - by Bioz Stars, 2026-10
90/100 stars
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nanowells coated with pll-g-peg  (Susos AG)
90
Susos AG nanowells coated with pll-g-peg
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Nanowells Coated With Pll G Peg, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/nanowells+coated+with+pll+g+peg/pmc10119611-372-13-14
Average 90 stars, based on 1 article reviews
nanowells coated with pll-g-peg - by Bioz Stars, 2026-10
90/100 stars
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azide-poly(ethylene glycol)-amine n3-peg-nh2  (NanoSoft LLC)
90
NanoSoft LLC azide-poly(ethylene glycol)-amine n3-peg-nh2
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Azide Poly(ethylene Glycol) Amine N3 Peg Nh2, supplied by NanoSoft LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/azide+functionalized+pll+g+peg/10__1016_slash_j__jddst__2024__106073-44-0-23
Average 90 stars, based on 1 article reviews
azide-poly(ethylene glycol)-amine n3-peg-nh2 - by Bioz Stars, 2026-10
90/100 stars
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pll- g -peg/peg-ggggygrgdsp pll- g -peg-rgd [pll(20)- g (3.5)-peg(2.3)/peg(3.4)-rgd]  (Susos AG)
90
Susos AG pll- g -peg/peg-ggggygrgdsp pll- g -peg-rgd [pll(20)- g (3.5)-peg(2.3)/peg(3.4)-rgd]
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll G Peg/Peg Ggggygrgdsp Pll G Peg Rgd [Pll(20) G (3.5) Peg(2.3)/Peg(3.4) Rgd], supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll++g++peg+peg+ggggygrgdsp+pll++g++peg+rgd/pmc05928488-117-19-38
Average 90 stars, based on 1 article reviews
pll- g -peg/peg-ggggygrgdsp pll- g -peg-rgd [pll(20)- g (3.5)-peg(2.3)/peg(3.4)-rgd] - by Bioz Stars, 2026-10
90/100 stars
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pll- g -peg/pegggggygrgdsp pll- g -peg-rgd-12  (Susos AG)
90
Susos AG pll- g -peg/pegggggygrgdsp pll- g -peg-rgd-12
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll G Peg/Pegggggygrgdsp Pll G Peg Rgd 12, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll++g++peg+pegggggygrgdsp+pll++g++peg+rgd+12/pmc06258691-209-17-35
Average 90 stars, based on 1 article reviews
pll- g -peg/pegggggygrgdsp pll- g -peg-rgd-12 - by Bioz Stars, 2026-10
90/100 stars
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poly-llysine(20)-grafted[3.5]-polyethyleneglycol(2) (pll-g-peg)  (Susos AG)
90
Susos AG poly-llysine(20)-grafted[3.5]-polyethyleneglycol(2) (pll-g-peg)
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Poly Llysine(20) Grafted[3.5] Polyethyleneglycol(2) (Pll G Peg), supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/poly+llysine+20++grafted+3+5++polyethyleneglycol+2+++pll+g+peg+/pm36709383-244-6-8
Average 90 stars, based on 1 article reviews
poly-llysine(20)-grafted[3.5]-polyethyleneglycol(2) (pll-g-peg) - by Bioz Stars, 2026-10
90/100 stars
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pll—g—peg co—polymer  (Susos AG)
90
Susos AG pll—g—peg co—polymer
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll—G—Peg Co—Polymer, supplied by Susos AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg+co+polymer/meletiou_anna__2021__imaging_cardiac_receptor_distributions_with_molecular_resolution_using_dna_paint-1209-11-12
Average 90 stars, based on 1 article reviews
pll—g—peg co—polymer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pll-peg  (NanoSoft LLC)
90
NanoSoft LLC pll-peg
A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and <t>PLL-PEG</t> prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on <t>poly-L-lysine,</t> fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.
Pll Peg, supplied by NanoSoft LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pll-g-peg/pll+g+peg/pmc07851858-216-27-28
Average 90 stars, based on 1 article reviews
pll-peg - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Reagents and tools table

Journal: The EMBO Journal

Article Title: An EpCAM/Trop2 mechanostat differentially regulates collective behaviour of human carcinoma cells

doi: 10.1038/s44318-024-00309-9

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Polylysine-PEG , JenKem Technology , PLL20K-G35-PEG2K , , .

Techniques: Control, Recombinant, Plasmid Preparation, Modification, Western Blot, Electron Microscopy, Saline

A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and PLL-PEG prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on poly-L-lysine, fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.

Journal: bioRxiv

Article Title: Actin filaments regulate microtubule growth at the centrosome

doi: 10.1101/302190

Figure Lengend Snippet: A - Schematic illustration of our model according to which cell spreading sequesters monomeric actin to the cortex and thereby enables the centrosome to grow more microtubules. Drawings show top (top line) and side views (bottom line) of cells with increased spreading from left to right. Actin filaments are in green, microtubules in red. B - RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and PLL-PEG prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars represent 10 μm and 2 μm, respectively) and α-tubulin (bottom line. Scale bar represents 10 μm). C - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in B. Measurements came from 3 independent experiments with more than 60 analyzed cells in each. Errors bars represent standard deviations. D - IIA1.6 B lymphoma cells were plated for 60 min on poly-L-lysine, fibronectin or ICAM-1 coated cover slides prior to be fixed and stained for F-actin (top line) and α-tubulin (bottom line). Scale bar represents 3 μm. E - Quantification of the area occupied by the cell on the substrate (top), centrosomal F-actin content (middle) and total amount of polymerized tubulin (bottom) for the three conditions of cell adhesion described in D. Measurements came from 3 independent experiments with more than 80 analyzed cells in each. Errors bars represent standard deviations.

Article Snippet: For the coating of glass coverslips; fibronectin (Sigma Aldrich) was used at 10 μg/ml and PLL-PEG (JenKem Technologies, Texas) at 10 μg/ml in Hepes 10 μM, Poly-L-Lysine (Invitrogen) was used at 10 μg/mL, and ICAM-1 (R&D System) was used at 10 μg/mL.

Techniques: Stable Transfection, Expressing, Staining