Journal: PLoS ONE

Article Title: Charge Shielding of PIP 2 by Cations Regulates Enzyme Activity of Phospholipase C

doi: 10.1371/journal.pone.0144432

Figure Lengend Snippet: Activity of PLCγ1 (A, B) and PLCδ1 (C, D) was measured as PLCβ1 in . Symbols and lines are experimental data and fitting with the mathematical model, respectively. n = 4–8 for PLCγ1 and n = 2–8 for PLCδ1.

Article Snippet: Recombinant human PLCβ1 and PLCδ1 proteins were purchased from OriGene (Rockville, MD) and human PLCγ1 was kindly provided by from Dr. John Sondek (University of North Carolina).

Techniques: Activity Assay

Journal: Frontiers in Molecular Neuroscience

Article Title: Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease

doi: 10.3389/fnmol.2013.00042

Figure Lengend Snippet: List of genes and their annotations included in the RT-qPCR array .

Article Snippet: Plcb1 , phospholipase C, beta 1 , Mm00479987_m1.

Techniques: Binding Assay

Journal: NPJ Schizophrenia

Article Title: Isoform specific differences in phospholipase C beta 1 expression in the prefrontal cortex in schizophrenia and suicide

doi: 10.1038/s41537-017-0020-x

Figure Lengend Snippet: PLCB1 a and b mRNA ( a ), and PLCB1a ( b ) and PLCB1b ( c ) protein, levels measured in CNS from Chrm1, 2, 4 and 5 knockout ( −/− ) mice compared to WT mice. Error bars show median (Mdn) and IQR. Western blot image ( d ) of 5 ug human or mouse cortical homogenate, run in duplicate, probed with anti-PLCB1 antibody

Article Snippet: Bands were then detected using mouse anti-PLCB1 antibody (Cat #610924, BD Biosciences) followed by HRP conjugated goat anti-mouse IgG (Cat #554002, BD Biosciences).

Techniques: Knock-Out, Western Blot