Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of almond skin extract (AE) and CHL on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Control, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of AE and CHL on SIRT1/SIR2 ( a ) and total SOD ( b ) enzyme activities. Y-CTL was young yeast (on day 2 of cultivation) and A-CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. In young yeast culture (Y), actual SIRT1/SIR2 enzyme activity was equal to 4564.4 FU/mg protein, whereas total SOD activity was 35.6 units/mg protein. The enzyme activity was expressed as a percentage of Y-CTL. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Control, Activity Assay, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Survival and ROS/RNS production under UV-C-induced oxidative stress conditions. ( a ) Survival assay, plates were incubated at 28 °C for 3 d and the number of colonies was counted; excepted if mentioned CTL was DBY746 yeast subjected to UV-C stress. ( b ) ROS/RNS production was evaluated using the dihydrorhodamine 123 (DHR123) probe. AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Clonogenic Cell Survival Assay, Incubation, Control, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Schematic view of the different ROS/RNS-induced oxidative damages to membrane lipids, proteins and DNA ( a ), and effects of AE and CHL on malondialdehyde (MDA) levels measured by TBARS assay ( b ), protein carbonyl contents determined by ELISA assay ( c ) and 8-oxo-Guanine formation determined by ELISA assay ( d ). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Membrane, TBARS Assay, Enzyme-linked Immunosorbent Assay, Control, Extraction

Journal: Journal of Advanced Pharmaceutical Sciences

Article Title: Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method

doi: 10.21608/japs.2024.290405.1027

Figure Lengend Snippet: Fig.1: The identified flavonolignans; silybin, isosilybin, silydianin, taxifolin, silychristin and silyamandin

Article Snippet: Taxifolin, and silydianin standards were ordered from LGC standards, UK.

Techniques:

Journal: bioRxiv

Article Title: Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring

doi: 10.64898/2026.01.22.701057

Figure Lengend Snippet: Data show the mean ± SD with each sample as dot plots for the protein abundance of phospholamban (PLN, B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I (cTnI, E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.

Article Snippet: Membranes were incubated with primary antibodies (PLN [1/5000 dilution] – MA3-922, InVitrogen; phospho-PLN-T17 [1/1000 dilution] – AP0910, ABclonal; cTnI [1/1000 dilution] – MCA1208, Bio-Rad; phospho-cTnI [1/1000 dilution] – MCA2780, Bio-Rad; Calsequestrin-1 [1/5000 dilution] – PA1-913, InVitrogen; SERCA2 [1/2000 dilution] – ab3625, Abcam; NCX1 [1/1000 dilution] – R3F1, Swant; LTCC [1/1000 dilution] – ab2864, Abcam; RYR2 [1/1000 dilution] – ab302716, Abcam; phospho-RYR2-S2808 [1/1000 dilution] – PA5-105712, InVitrogen) overnight at 4°C, before secondary antibody incubation (IRDye® 800CW Goat anti-mouse for PLN, cTnI, phospho-cTnI, NCX1 and LTCC, IRDye® 800CW goat anti-rabbit for phospho-PLN-T17, Calsequestrin-1, SERCA2, RYR2 and phosphor-RYR2-S2808, both 1/20 000 dilution) at room temperature for one hour.

Techniques: Quantitative Proteomics, MANN-WHITNEY