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Image Search Results
Journal: Cell & bioscience
Article Title: Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.
doi: 10.1186/s13578-022-00845-z
Figure Lengend Snippet: Fig. 3 PF4 induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
Article Snippet: PF4, thrombin-antithrombin (TAT) complex and von Willebrand factor (VWF) in plasma samples were detected by TAT complex ELISA kit (Elabscience, Shanghai),
Techniques: Incubation, Staining, Clinical Proteomics, Control, Recombinant, Flow Cytometry
Journal: Cell & bioscience
Article Title: Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.
doi: 10.1186/s13578-022-00845-z
Figure Lengend Snippet: Fig. 4 NET markers were colocalized with autophagy associated protein. a–c CVST thrombi were stained with CD66b (red), citH3 (green), PF4 (pink), Beclin-1 (pink) and LC3B (pink). The inset scale bars are 20 μm in a-c
Article Snippet: PF4, thrombin-antithrombin (TAT) complex and von Willebrand factor (VWF) in plasma samples were detected by TAT complex ELISA kit (Elabscience, Shanghai),
Techniques: Staining
Journal: Materials Today Bio
Article Title: Delivered baicalein immunomodulatory hydrogel with dual properties of pH-responsive and anti-infection orchestrates pro-regenerative response of macrophages for enhanced hypertrophic scars therapy
doi: 10.1016/j.mtbio.2025.102160
Figure Lengend Snippet: Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of CD31 (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Following seeding in 12-well culture plates, HUVECs were maintained for 72 h in experimental media supplemented with different test materials, after which the supernatants were collected, centrifuged, and analyzed for
Techniques: Functional Assay, Wound Healing Assay, Migration, Enzyme-linked Immunosorbent Assay, Expressing, In Vivo