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Elabscience Biotechnology pf4 elisa kit
Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
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Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
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Elabscience Biotechnology mouse pf4 elisa kit
Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
Mouse Pf4 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane anti mouse thrombocyte antiserum polyclonal rabbit serum
Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
Anti Mouse Thrombocyte Antiserum Polyclonal Rabbit Serum, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech cd36 ab252923
Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
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Beijing Solarbio Science pig peripheral blood lymphocyte separation solution kit
Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
Pig Peripheral Blood Lymphocyte Separation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3 <t>PF4</t> induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils
Pdgf Bb, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology cd31
Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of <t>CD31</t> (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Cd31, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology human pf4 platelet factor 4 elisa kit
Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of <t>CD31</t> (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Human Pf4 Platelet Factor 4 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 3 PF4 induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils

Journal: Cell & bioscience

Article Title: Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.

doi: 10.1186/s13578-022-00845-z

Figure Lengend Snippet: Fig. 3 PF4 induces NET formation in CVST patients. a NPAs (CD41+/CD66b+) were examined in samples from healthy controls (n = 20) and CVST (n = 17) patients. b–e Neutrophils (from healthy people) were incubated with PRP from patients and healthy subjects and stained with CD41 (red) and MPO (green) to show the colocalization of NETs and PLTs. f Neutrophils (from CVST patients) were incubated with PRP from healthy subjects and stained with CD41 (red) and MPO (green). g Plasma PF4 levels were measured in samples from CVST patients (n = 37) and healthy subjects (n = 32). h Control neutrophils were treated with different concentrations of recombinant PF4 protein. CitH3-DNA was measured in each group (n = 10). Control neutrophils were incubated with PRP from healthy subjects and CVST patients in the presence of anti-PF4 antibodies. i NET releasing neutrophils (citH3 + Neut) was measured in cells from each group by flow cytometry. j–o Neutrophils were stained with MPO (red) and citH3 (green). Statistics: Ordinary one-way ANOVA (a, g); Brown-Forsythe and Welch’s ANOVA test (f, h, i). The inset scale bars are 20 μm in b–e, j–o. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Neut, Neutrophils

Article Snippet: PF4, thrombin-antithrombin (TAT) complex and von Willebrand factor (VWF) in plasma samples were detected by TAT complex ELISA kit (Elabscience, Shanghai), PF4 ELISA kit (Elabscience, Shanghai) and vWF ELISA kit (Elabscience, Shanghai), respectively.

Techniques: Incubation, Staining, Clinical Proteomics, Control, Recombinant, Flow Cytometry

Fig. 4 NET markers were colocalized with autophagy associated protein. a–c CVST thrombi were stained with CD66b (red), citH3 (green), PF4 (pink), Beclin-1 (pink) and LC3B (pink). The inset scale bars are 20 μm in a-c

Journal: Cell & bioscience

Article Title: Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.

doi: 10.1186/s13578-022-00845-z

Figure Lengend Snippet: Fig. 4 NET markers were colocalized with autophagy associated protein. a–c CVST thrombi were stained with CD66b (red), citH3 (green), PF4 (pink), Beclin-1 (pink) and LC3B (pink). The inset scale bars are 20 μm in a-c

Article Snippet: PF4, thrombin-antithrombin (TAT) complex and von Willebrand factor (VWF) in plasma samples were detected by TAT complex ELISA kit (Elabscience, Shanghai), PF4 ELISA kit (Elabscience, Shanghai) and vWF ELISA kit (Elabscience, Shanghai), respectively.

Techniques: Staining

Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of CD31 (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Delivered baicalein immunomodulatory hydrogel with dual properties of pH-responsive and anti-infection orchestrates pro-regenerative response of macrophages for enhanced hypertrophic scars therapy

doi: 10.1016/j.mtbio.2025.102160

Figure Lengend Snippet: Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of CD31 (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Following seeding in 12-well culture plates, HUVECs were maintained for 72 h in experimental media supplemented with different test materials, after which the supernatants were collected, centrifuged, and analyzed for CD31 (E-EL-H6227, Elabscience) and VEGF (E-EL-H0111, Elabscience) concentrations.

Techniques: Functional Assay, Wound Healing Assay, Migration, Enzyme-linked Immunosorbent Assay, Expressing, In Vivo