Journal: Epilepsia Open

Article Title: High FGF‐21 level in a cohort of 22 patients with Dravet Syndrome—Possible relationship with the disease outcomes

doi: 10.1002/epi4.12534

Figure Lengend Snippet: Comparison of plasma FGF‐21 concentrations (pg/mL) in patients with Dravet syndrome (DS) and normal control. Mean of log 10 FGF‐21 level were significantly higher in DS patients comparing with normal controls by unpaired t ‐test with 2‐tailed P ‐values <.01**. Horizontal lines indicated mean of log 10 FGF‐21

Article Snippet: Plasma FGF‐21 concentrations were measured in duplicate by enzyme‐linked immunosorbent assay (ELISA) for FGF‐21 (BioVendor, Brno, Czech Republic) according to the manufacturer’s instructions.

Techniques:

Journal: Epilepsia Open

Article Title: High FGF‐21 level in a cohort of 22 patients with Dravet Syndrome—Possible relationship with the disease outcomes

doi: 10.1002/epi4.12534

Figure Lengend Snippet: Comparison of plasma FGF‐21 concentrations (pg/mL) and seizure frequency in patients with Dravet syndrome (DS) (A) Log 10 FGF‐21 level in DS patients with mild‐to‐normal versus moderate‐to‐severe intellectual disabilities (ID); (B) Log 10 FGF‐21 in DS patients with versus without valproate treatment; (C) Log 10 FGF‐21 level in DS patients with missense versus truncating SCN1A variants; (D) Seizure per month in DS patients with versus without valproate treatment. Mean of log 10 FGF‐21 level of the two groups were compared using unpaired t ‐test and 2‐tailed P ‐values <.05* were considered significant. Horizontal lines indicated mean of log 10 FGF‐21

Article Snippet: Plasma FGF‐21 concentrations were measured in duplicate by enzyme‐linked immunosorbent assay (ELISA) for FGF‐21 (BioVendor, Brno, Czech Republic) according to the manufacturer’s instructions.

Techniques:

Journal: Epilepsia Open

Article Title: High FGF‐21 level in a cohort of 22 patients with Dravet Syndrome—Possible relationship with the disease outcomes

doi: 10.1002/epi4.12534

Figure Lengend Snippet: Correlation of seizure frequency and plasma FGF‐21 concentrations in patients with Dravet syndrome (DS)

Article Snippet: Plasma FGF‐21 concentrations were measured in duplicate by enzyme‐linked immunosorbent assay (ELISA) for FGF‐21 (BioVendor, Brno, Czech Republic) according to the manufacturer’s instructions.

Techniques:

Journal: Endocrine Journal

Article Title: Effects of sodium-glucose cotransporter 2 inhibitor (dapagliflozin) on food intake and plasma fibroblast growth factor 21 levels in type 2 diabetes patients

doi: 10.1507/endocrj.ej19-0013

Figure Lengend Snippet: Fig. 1 Correlation between FGF21 and BMI at baseline. FGF21, fibroblast growth factor 21; BMI, body mass index.

Article Snippet: Plasma FGF21 concentrations were measured by ELISA (FGF21 Quantikine ELISA kit; R&D Systems, Minneapolis, MN, USA).

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Journal: Biochemical and biophysical research communications

Article Title: Growth hormone resistance induced by amino acid deprivation in fao cells is independent of FGF21.

doi: 10.1016/j.bbrc.2024.149811

Figure Lengend Snippet: Fig. 3. Effect of FGH21 addition on GH signaling. A, B) Immunoblotting of JAK2, p-JAK2, STAT5b, and HSP90. Fao cells were cultured in the Full or Zero medium with 0–10 [5] pM of recombinant FGF21 for 24 h. Cells were then treated with 100 nM GH or vehicle for 10 min. C) Igf1 mRNA levels were quantified using real-time qPCR. Values were normalized against the Actb mRNA level. Fao cells were cultured in the full medium with or without 100 nM GH and 0–105 pM of FGF21 for 24 h. Total RNA was isolated. Bar: means ± SEM (n = 3), *p < 0.05. (D) The expression of α-klotho (Kl), β-klotho (Klb), and FGF Receptor (Fgfr) 1–4 in Fao cells, rat primary hepatocytes, and rat tissue samples were analyzed using reverse transcription-PCR. (E) Immnoblotting of Erk and p-Erk. Fao cells were cultured in the full medium for 24 h. The cells were then treated with 0–104 pM of FGF21 or vehicle for 10 min.

Article Snippet: Plasma FGF21 concentrations were measured using a mouse/rat FGF21 ELISA kit (Proteintech, IL, USA).

Techniques: Western Blot, Cell Culture, Recombinant, Isolation, Expressing, Reverse Transcription

Journal: Scientific Reports

Article Title: CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis

doi: 10.1038/srep27938

Figure Lengend Snippet: ( A ) Correlation between hepatic TG and FGF21 mRNA levels on Log 2 scale in mice. Each symbol represents an individual mouse as indicated. R 2 = 0.1995, P (two-tailed) = 0.0056. ( B ) Hepatic FGF21 mRNA levels determined by qRT-PCR (n = 4 per group). ( C ) Plasma FGF21 levels measured after a 16 h fast (n = 5 per group). ( D ) Hepatic FGF21 mRNA levels (n = 4 per group). ( E ) Plasma FGF21 protein levels (n = 7 per group). ( F ) Total FGF21 concentration in the conditioned media of the primary hepatocytes infected by Ad.GFP, Ad.CREBH( N ) or Ad.hFGF21. Scale bar = 200 μm. ( G ) Ex vivo lipolysis assay. Relative amount of glycerol released from WAT slice cultured in the presence of conditioned media for 3 hours. Isoproterenol (100 nM) was added to culture media to measure stimulated lipolysis. The results are the average of 5–6 independent experiments. A paired t-test was performed for statistical analysis. Data are shown as mean ± s.e.m. * P < 0.05, ** P < 0.01.

Article Snippet: Human FGF21 adenovirus was purchased from Vector Biolabs.

Techniques: Two Tailed Test, Quantitative RT-PCR, Clinical Proteomics, Concentration Assay, Infection, Ex Vivo, Cell Culture

Journal: Scientific Reports

Article Title: CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis

doi: 10.1038/srep27938

Figure Lengend Snippet: ( A ) Creb3l3 −/− mice were infected by Ad.GFP or Ad.hFGF21. Plasma FGF21 levels were measured 2 days after the virus infection (n = 4 per group). ( B ) Plasma glycerol and ( C ) NEFA levels in the adenovirus infected mice measured at days 0, 2 and 4 on KD (n = 4 per group). ( D ) Adipose tissues were isolated from adenovirus-infected Creb3l3 −/− mice fed KD for 2 days, and analyzed for HSL and perilipin phosphorylation by western blotting. ( E ) Hepatic TG levels. ( F ) Oil red O staining of liver sections. ( G ) Plasma TG and ( H ) ALT levels (n = 4 per group). ( I ) Hepatic mRNA levels determined by qRT-PCR (n = 4 per group). Data are shown as mean ± s.e.m. * P < 0.05 and ** P < 0.01.

Article Snippet: Human FGF21 adenovirus was purchased from Vector Biolabs.

Techniques: Infection, Clinical Proteomics, Virus, Isolation, Phospho-proteomics, Western Blot, Staining, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis

doi: 10.1038/srep27938

Figure Lengend Snippet: CREBH is activated in fatty livers and promotes FGF21 production. FGF21 suppresses lipolysis, alleviating mobilization of TG from adipose tissue to the liver. CREBH also induces apoA-IV, apoA-V and apoC-II that stimulate LPL-mediated TG clearance. Loss of CREBH causes hepatic steatosis and hypertriglyceridemia.

Article Snippet: Human FGF21 adenovirus was purchased from Vector Biolabs.

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