Journal: Plant biology (Stuttgart, Germany)

Article Title: The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance.

doi: 10.1111/plb.13711

Figure Lengend Snippet: Fig. 2. Analysis of OsS1Fa1 and OsS1Fa2 expression in rice tissues and detection of the His-tagged recombinant proteins in E. coli. (A) A comparison of the deduced amino acid sequences of OsS1Fa1 and OsS1Fa2. (B) Amino acid sequences of conserved motifs in OsS1Fa1 and OsS1Fa2 are indicated. (C) Total RNA was extracted from leaf, culm, and root tissues of 3-week-old rice seedlings, and the expression levels of OsS1Fa1 and OsS1Fa2 were analysed using qRT-PCR. Data represent mean SD (n = 3). (D) Detection of recombinant His6-OsS1Fa1 and His6-OsS1Fa2 proteins with anti-OsS1Fa1 antibody.

Article Snippet: Rice leaf, culm, and root samples were thoroughly ground, and total RNA was extracted from the ground tissue using the FavorPrepTM Plant Total RNA Mini Kit (Favorgen).

Techniques: Expressing, Recombinant, Comparison, Quantitative RT-PCR

Journal: Plant biology (Stuttgart, Germany)

Article Title: The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance.

doi: 10.1111/plb.13711

Figure Lengend Snippet: Fig. 4. Drought tolerance assay of transgenic Arabidopsis overexpressing OsS1Fa1 and mutant proteins. (A) Examination of OsS1Fa1, OsS1Fa1(TMm1), OsS1Fa1(mNLS ), and OsS1Fa1(K63R) expression in OsS1Fa1-, OsS1Fa1(mNLS)-, OsS1Fa1(K63R)-, and OsS1Fa1(TMm1)-overexpressing Arabidopsis. Total RNA was extracted from the leaves of 15-day-old WT and transgenic Arabidopsis, and then OsS1Fa1, OsS1Fa1(TMm1), OsS1Fa1(mNLS ), OsS1Fa1(K63R) transcripts were examined by RT-PCR using OsS1Fa1- and FLAG-specific primers. (B) Seeds of WT, OsS1Fa1-, OsS1Fa1(mNLS)-, OsS1Fa1(K63R)-, and OsS1Fa1(TMm1)- overexpressing Arabidopsis were grown for 15 days in moist soil, and watering was withheld for 7 days for drought stress treatment. Following the drought stress treatment, the plants were rewatered, and photographs taken after an additional 3 and 5 days.

Article Snippet: Rice leaf, culm, and root samples were thoroughly ground, and total RNA was extracted from the ground tissue using the FavorPrepTM Plant Total RNA Mini Kit (Favorgen).

Techniques: Transgenic Assay, Mutagenesis, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Plant Science

Article Title: Identification of nuclear factor YA6 genes in sorghum and characterization of their involvement in drought tolerance

doi: 10.3389/fpls.2025.1524066

Figure Lengend Snippet: Subcellular localization of SbNF-YA6. Arabidopsis protoplasts were transfected with 35S:GFP (control) or 35S:SbNF-YA6 -GFP. The fluorescence signals were detected under a confocal microscope. Chlorophyll autofluorescence was used to visualize chloroplasts.

Article Snippet: A plant protoplast isolation kit (Beyotime, Beijing, China) was used to isolate protoplasts from Arabidopsis plants at the five- to six-leaf stage.

Techniques: Transfection, Control, Fluorescence, Microscopy

Journal: The Journal of Membrane Biology

Article Title: α-Tocopherol/Gallic Acid Cooperation in the Protection of Galactolipids Against Ozone-Induced Oxidation

doi: 10.1007/s00232-015-9851-4

Figure Lengend Snippet: Ozone concentration dependence of the ratio A / A 0 lift-off values of galactolipid layers contacted ozone containing subphase ( A ) to the value characteristic for the layer contacting ozone-free subphase ( A 0 ). a MGDG, b DGDG. Filled circles GA = 0; triangles 2 × 10 −6 M GA; diamonds 5 × 10 −6 M GA

Article Snippet: Galactolipids: mono- (MGDG) and digalactosyldiacylglycerol (DGDG) were from Avanti Polar Lipids Inc. (USA/Canada). α-Tocopherol (TOH) of ≥96 % purity and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) were obtained from Sigma.

Techniques: Concentration Assay

Journal: The Journal of Membrane Biology

Article Title: α-Tocopherol/Gallic Acid Cooperation in the Protection of Galactolipids Against Ozone-Induced Oxidation

doi: 10.1007/s00232-015-9851-4

Figure Lengend Snippet: Ozone concentration dependence of the ratio A / A 0 lift-off values of mixed galactolipid/tocopherol layers contacted ozone containing subphase—( A ) to the value corresponding the layer spread on ozone-free subphase ( A 0 ). a MGDG, b DGDG. Filled circles and solid lines GA = 0; triangles and dash lines 2 × 10 −6 M GA; diamonds and dotted lines 5 × 10 −6 M GA. Points marked as stars and dash–dot corresponding line represent results obtained for mixtures of galactolipids with pre-oxidized tocopherol

Article Snippet: Galactolipids: mono- (MGDG) and digalactosyldiacylglycerol (DGDG) were from Avanti Polar Lipids Inc. (USA/Canada). α-Tocopherol (TOH) of ≥96 % purity and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) were obtained from Sigma.

Techniques: Concentration Assay

Journal: The Journal of Membrane Biology

Article Title: α-Tocopherol/Gallic Acid Cooperation in the Protection of Galactolipids Against Ozone-Induced Oxidation

doi: 10.1007/s00232-015-9851-4

Figure Lengend Snippet: The differences in ozone concentrations shift ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \Delta c_{{{\text{O}}_{3} }} $$\end{document} Δ c O 3 ) corresponding to the half height of curves obtained for two levels of GA in subphase divided by maximal possible ozone level decrease (assuming complete GA mineralization)

Article Snippet: Galactolipids: mono- (MGDG) and digalactosyldiacylglycerol (DGDG) were from Avanti Polar Lipids Inc. (USA/Canada). α-Tocopherol (TOH) of ≥96 % purity and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) were obtained from Sigma.

Techniques:

Journal: The Journal of Membrane Biology

Article Title: α-Tocopherol/Gallic Acid Cooperation in the Protection of Galactolipids Against Ozone-Induced Oxidation

doi: 10.1007/s00232-015-9851-4

Figure Lengend Snippet: Compressibility modulus 1/ β as a function of surface pressure π of layers: galactolipid— solid line , mixed galactolipid/TOH— short dash , galactolipid/pre-oxidized tocopherol— dotted , spread onto subphase not containing any ozone (PBS, pH 7.0). a MGDG, b DGDG

Article Snippet: Galactolipids: mono- (MGDG) and digalactosyldiacylglycerol (DGDG) were from Avanti Polar Lipids Inc. (USA/Canada). α-Tocopherol (TOH) of ≥96 % purity and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) were obtained from Sigma.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Growth Temperature Influence on Lipids and Photosynthesis in Lepidium sativum

doi: 10.3389/fpls.2020.00745

Figure Lengend Snippet: Profile of the galacto- and prenyl-lipids in Lepidium sativum grown at different temperatures. Total lipids were extracted from pooled leaf samples, separated by liquid chromatography and identified by mass spectrometry. Eleven leaf samples from three independent growth replicates were used. Relative concentration of the principal antioxidants of the thylakoid membrane (A) α-tocopherol, (B) γ-tocopherol, (C) plastochromanol, and its precursors (D) plastoquinone, the latter also serves as an electron carrier in the electron transport chain. In the box plots, the lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The whiskers extend from the hinge to the farthest value no further than 1.5 times the distance between the first and third quartiles from the hinge. Farther points are considered as “outliers” and plotted individually. The p -value derived from a post hoc test comparing the data with the value at 22°C is shown above each box. (E) Profile of the monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG) expressed as % molecules over the total of the measured galactolipids. The compounds are listed by the length and degree of saturation of the acyl chains, and significant differences from the control temperature 22°C ( p < 0.05) are highlighted with an asterisk ( n = 11).

Article Snippet: MGDG 36:6, 36:5, 36:4, 34:6, 34:5, 34:4 and DGDG 36:6, 36:5, 36:4, 36:3, 34:6, 34:3, 34:2, 34:1 identities were confirmed comparing the peak of the extract with a standard plant MGDG mix and DGDG mix (Avanti Polar Lipids).

Techniques: Liquid Chromatography, Mass Spectrometry, Concentration Assay, Membrane, Derivative Assay, Control