Journal: Journal of Nanobiotechnology

Article Title: Gouqi-derived nanovesicles (GqDNVs) inhibited dexamethasone-induced muscle atrophy associating with AMPK/SIRT1/PGC1α signaling pathway

doi: 10.1186/s12951-024-02563-9

Figure Lengend Snippet: The effect of GqDNVs on C2C12 cells. a Picture of C2C12 cells and GqDNVs through fluorescence confocal microscopy. PKH 26 marked GqDNVs (red fluorescent); FITC stained cytoskeleton of C2C12 cells (green fluorescent); DAPI stained the nucleus of C2C12 cells (blue fluorescent); the merged diagram was a coincidence diagram of the three fluorescent images. b , c Cell viability test of GqDNVs. “CON” means C2C12 cells were treated with PBS; “100 μM” means the administration of 100 μM dexamethasone; “GqDNV1-5” means the administration of different concentrations of GqDNVs (1 × 10 9 , 5 × 10 8 , 2.5 × 10 8 , 1 × 10 8 , 5 × 10 7 particles/mL). “*” indicates that there is a significant difference compared with the 100 μM dexamethasone intervention group ( P < 0.05) after the two-sample t-test analysis. d The concentration of ATP in C2C12 cells. e The representative images of diameters of myotubes in C2C12 cells. Scale bars show 50 μm. f Quantitative analyses of the mean diameter of myotubes [mean ± standard deviation (SD)] and diameter distribution of myotubes of C2C12 cells. d – f One-way ANOVA analysis and Tukey’s multiple comparison analysis were used in the between-group comparisons. “*”, “**”, “***” and “****” indicate that after Tukey’s multiple comparison analysis, the P -value is lower than 0.05, 0.01, 0.001 and 0.0001

Article Snippet: GqDNVs were labeled with PKH 26 (red fluorescent, MedChemExpress, China).

Techniques: Fluorescence, Confocal Microscopy, Staining, Concentration Assay, Standard Deviation, Comparison

Journal: British Journal of Cancer

Article Title: Efficacy of immunoliposomes on cancer models in a cell-surface-antigen-density-dependent manner

doi: 10.1038/sj.bjc.6601341

Figure Lengend Snippet: Internalisation of ILD. Cultured B37 cells were reacted with PKH2-labelled ILD for 1 h at 4°C without ( A ) or with free F(ab′) 2 /GAH at a concentration of 0.5 mg ml −1 ( B ), about 100 times the amount of antibody bound on liposomes, as well as with free human IgG F(ab′) 2 ( C ) for a control. After another 1 h incubation at 37°C, the cells were washed and fixed and then examined under a confocal fluorescence microscope. Scale bar: 10 μ m.

Article Snippet: ILD was labelled with PKH2 (Zynaxis Cell Science, Inc., Malvern, PA, USA), which emits green fluorescence and has a high affinity for the lipid bilayers generally used for membrane labelling ( Horan et al , 1990 ).

Techniques: Cell Culture, Concentration Assay, Liposomes, Control, Incubation, Fluorescence, Microscopy