Journal: Frontiers in Cellular Neuroscience

Article Title: Long-Term Cultures of Spinal Cord Interneurons

doi: 10.3389/fncel.2022.827628

Figure Lengend Snippet: List of antibodies used for immunocytochemistry.

Article Snippet: PKC γ , , 1/100 , , Santa Cruz , sc-166385.

Techniques: Immunocytochemistry

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Diacylglycerol kinase γ facilitates the proliferation and migration of neural stem cells in the developing neural tube

doi: 10.3724/abbs.2024156

Figure Lengend Snippet: Immunofluorescence staining showing the coexpression of DGKγ with p-PKCγ or p-PKCδ DGKγ was coexpressed with p-PKCγ (A) and p-PKCδ (B) in the cytoplasm and nucleus of neuroepithelial NT cells at E11.5. Blue: DAPI; red: DGKγ; green: p-PKCγ or p-PKCδ. Scale bar: 100 μm. n = 5.

Article Snippet: The PVDF membranes were subsequently incubated with primary antibodies against DGKγ (1:500, ab89037; Abcam, Cambridge, UK), p-PKCδ (1:1000, sc-365969; Santa Cruz Biotech), PKCδ (1:1000, sc-213; Santa Cruz Biotech), p-PKCγ (1:1000, 44-975G; Invitrogen), PKCγ (1:1000, BM5623; Boster), and β-actin (1:4000, AP0060; Bioworld Technology, Shanghai, China) overnight at 4°C.

Techniques: Immunofluorescence, Staining

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Diacylglycerol kinase γ facilitates the proliferation and migration of neural stem cells in the developing neural tube

doi: 10.3724/abbs.2024156

Figure Lengend Snippet: Changes in DAG content and p-PKC subtypes during NSC migration (A) DAG contents were determined by ELISA, which revealed that the DAG contents were significantly increased in sh-DGKγ-treated cells compared with those in the sh-NC-treated cells and significantly increased in R59949- and PMA-treated cells compared with those in the vehicle-treated cells. (B) Representative western blots of p-PKCγ and p-PKCδ in NSCs were quantified via western blotting. (C,D) Densitometric analysis of the western blots revealed that the p-PKCγ/PKCγ ratio was significantly lower in the PMA group than in the vehicle group; however, the p-PKCδ/PKCδ ratio was significantly greater in the sh-DGKγ group than in the sh-NC group, which was also significantly greater in the R59949 and PMA groups than in the vehicle group. *P < 0.05, **P < 0.01, ***P < 0.001 vs sh-NC or vehicle. n = 3.

Article Snippet: The PVDF membranes were subsequently incubated with primary antibodies against DGKγ (1:500, ab89037; Abcam, Cambridge, UK), p-PKCδ (1:1000, sc-365969; Santa Cruz Biotech), PKCδ (1:1000, sc-213; Santa Cruz Biotech), p-PKCγ (1:1000, 44-975G; Invitrogen), PKCγ (1:1000, BM5623; Boster), and β-actin (1:4000, AP0060; Bioworld Technology, Shanghai, China) overnight at 4°C.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Diacylglycerol kinase γ facilitates the proliferation and migration of neural stem cells in the developing neural tube

doi: 10.3724/abbs.2024156

Figure Lengend Snippet: Schematic diagram of the role of DGKγ in NSC migration and proliferation A schematic diagram showing that the DAG/PKCδ signaling pathway is involved in NSC migration and that the mTOR signaling pathway, independent of PKC, may be involved in NSC proliferation.

Article Snippet: The PVDF membranes were subsequently incubated with primary antibodies against DGKγ (1:500, ab89037; Abcam, Cambridge, UK), p-PKCδ (1:1000, sc-365969; Santa Cruz Biotech), PKCδ (1:1000, sc-213; Santa Cruz Biotech), p-PKCγ (1:1000, 44-975G; Invitrogen), PKCγ (1:1000, BM5623; Boster), and β-actin (1:4000, AP0060; Bioworld Technology, Shanghai, China) overnight at 4°C.

Techniques: Migration