pjak1 Search Results


pjak1 mfi  (Mendeley Ltd)
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Mendeley Ltd pjak1 mfi
Pjak1 Mfi, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-pjak1 at tyr-1022 pab  (ImmunoWay Biotechnology Company)
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ImmunoWay Biotechnology Company anti-pjak1 at tyr-1022 pab
Anti Pjak1 At Tyr 1022 Pab, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody rabbit anti-pjak1  (Wanleibio)
90
Wanleibio antibody rabbit anti-pjak1
Antibody Rabbit Anti Pjak1, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-pjak1 (tyr 1022/1023)  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc anti-pjak1 (tyr 1022/1023)
IL-15 signal transduction: IL-15R/GM-CSFR cross talk in TF1β cells. Analysis of JAK/STAT signal transduction by Western blotting. TF1β cells were incubated with 10 ng/ml rIL-15 for 15 min at 37°C. Sister cultures were pretreated for 1 h with neutralizing anti–IL-15Rβ/γc, anti-GM-CSFRβ, or anti-IL-6R gp130 mAbs. Cell extracts were analyzed by Western blotting using anti-phospho-JAK1 <t>(pJAK1),</t> anti-phospho-JAK2 (pJAK2), anti-phospho-TYK2 (pTYK2), anti-phospho-STAT5 (pSTAT5), and anti-phospho-STAT6 (pSTAT6) antibodies. Membranes were then reprobed with antibodies recognizing the native proteins. To correct for possible variations in the amount of protein loaded, values are expressed as pJAK/JAK or pSTAT/STAT ratios. pJAK/JAK and pSTAT/STAT levels were determined by densitometry including correction for background (NIH Image software). Results are expressed as increases (e.g., two times) with respect to untreated cells. The data presented are representative of three independent experiments.
Anti Pjak1 (Tyr 1022/1023), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-pjak1 (tyr 1022/1023)/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
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rabbit anti-pjak1-py1022 polyclonal mbs821826  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rabbit anti-pjak1-py1022 polyclonal mbs821826
IL-15 signal transduction: IL-15R/GM-CSFR cross talk in TF1β cells. Analysis of JAK/STAT signal transduction by Western blotting. TF1β cells were incubated with 10 ng/ml rIL-15 for 15 min at 37°C. Sister cultures were pretreated for 1 h with neutralizing anti–IL-15Rβ/γc, anti-GM-CSFRβ, or anti-IL-6R gp130 mAbs. Cell extracts were analyzed by Western blotting using anti-phospho-JAK1 <t>(pJAK1),</t> anti-phospho-JAK2 (pJAK2), anti-phospho-TYK2 (pTYK2), anti-phospho-STAT5 (pSTAT5), and anti-phospho-STAT6 (pSTAT6) antibodies. Membranes were then reprobed with antibodies recognizing the native proteins. To correct for possible variations in the amount of protein loaded, values are expressed as pJAK/JAK or pSTAT/STAT ratios. pJAK/JAK and pSTAT/STAT levels were determined by densitometry including correction for background (NIH Image software). Results are expressed as increases (e.g., two times) with respect to untreated cells. The data presented are representative of three independent experiments.
Rabbit Anti Pjak1 Py1022 Polyclonal Mbs821826, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-pjak1-py1022 polyclonal mbs821826/product/MyBiosource Biotechnology
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Image Search Results


IL-15 signal transduction: IL-15R/GM-CSFR cross talk in TF1β cells. Analysis of JAK/STAT signal transduction by Western blotting. TF1β cells were incubated with 10 ng/ml rIL-15 for 15 min at 37°C. Sister cultures were pretreated for 1 h with neutralizing anti–IL-15Rβ/γc, anti-GM-CSFRβ, or anti-IL-6R gp130 mAbs. Cell extracts were analyzed by Western blotting using anti-phospho-JAK1 (pJAK1), anti-phospho-JAK2 (pJAK2), anti-phospho-TYK2 (pTYK2), anti-phospho-STAT5 (pSTAT5), and anti-phospho-STAT6 (pSTAT6) antibodies. Membranes were then reprobed with antibodies recognizing the native proteins. To correct for possible variations in the amount of protein loaded, values are expressed as pJAK/JAK or pSTAT/STAT ratios. pJAK/JAK and pSTAT/STAT levels were determined by densitometry including correction for background (NIH Image software). Results are expressed as increases (e.g., two times) with respect to untreated cells. The data presented are representative of three independent experiments.

Journal: The Journal of Experimental Medicine

Article Title: Detection of a Functional Hybrid Receptor γc/GM-CSFRβ in Human Hematopoietic CD34 + Cells

doi: 10.1084/jem.20020150

Figure Lengend Snippet: IL-15 signal transduction: IL-15R/GM-CSFR cross talk in TF1β cells. Analysis of JAK/STAT signal transduction by Western blotting. TF1β cells were incubated with 10 ng/ml rIL-15 for 15 min at 37°C. Sister cultures were pretreated for 1 h with neutralizing anti–IL-15Rβ/γc, anti-GM-CSFRβ, or anti-IL-6R gp130 mAbs. Cell extracts were analyzed by Western blotting using anti-phospho-JAK1 (pJAK1), anti-phospho-JAK2 (pJAK2), anti-phospho-TYK2 (pTYK2), anti-phospho-STAT5 (pSTAT5), and anti-phospho-STAT6 (pSTAT6) antibodies. Membranes were then reprobed with antibodies recognizing the native proteins. To correct for possible variations in the amount of protein loaded, values are expressed as pJAK/JAK or pSTAT/STAT ratios. pJAK/JAK and pSTAT/STAT levels were determined by densitometry including correction for background (NIH Image software). Results are expressed as increases (e.g., two times) with respect to untreated cells. The data presented are representative of three independent experiments.

Article Snippet: Membranes were blocked by incubation with 5% BSA and probed with the following antibodies: anti-JAK1, anti-JAK2, and 4G10 anti-phosphotyrosine (Upstate Biotechnology/USA Euromodex), anti-pJAK1 (Tyr 1022/1023), anti-pJAK2 (Tyr 1007/Tyr 1008), anti-STAT3, anti-pSTAT3 (Tyr705), and anti-STAT6 (Santa Cruz Biotechnology, Inc.), anti-STAT5 (Transduction Laboratories/Becton Dickinson), anti-pSTAT5 (Tyr694), anti-pSTAT6 (Tyr641; Cell Signaling/New England Biolabs, Inc.) and anti-pIκBα (Calbiochem).

Techniques: Transduction, Western Blot, Incubation, Software