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Image Search Results
Journal: Diabetes
Article Title: X-Box Binding Protein 1 Is Essential for Insulin Regulation of Pancreatic α-Cell Function
doi: 10.2337/db12-1747
Figure Lengend Snippet: XBP1 deficiency impairs insulin signaling. Western blotting for insulin receptor (IR) tyrosine 972 (Y) phosphorylation (pIR), IR, IRS1 tyrosine 896 (Y) phosphorylation, IRS1 Ser 307 (Ser) phosphorylation, total IRS1, Akt Thr 308 phosphorylation, Akt Ser 473 phosphorylation, total Akt, and β-actin (loading control) in control or αXBPKD cell lines ( A ) and in control or αXBPOE cell lines ( D ) before and after insulin (ins) treatment for 10 min (100 nmol/L). Quantification of pIRS1(Y)/IRS1, pIRS1(Ser)/IRS1, pAkt(T308)/Akt, and pAkt(S473)/Akt in control or αXBPKD cell lines ( B ) and in control or αXBPOE cell lines ( E ). Data are expressed as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Glucagon secretion from control or αXBPKD cell lines ( C ) and control or αXBPOE cell lines ( F ) was assessed by static incubation for 60 min under various glucose concentrations, without or with 100 nmol/L insulin (Ins), and expressed per microgram of total DNA ( n = 3–6 in each group). Data are expressed as means ± SEM. * P < 0.05, ** P < 0.01; n.s., not significant.
Article Snippet: Binding immunoglobulin protein (BiP), IRE1α, pPERK, pJNK, JNK,
Techniques: Western Blot, Phospho-proteomics, Control, Incubation
Journal: BMC Complementary and Alternative Medicine
Article Title: Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats
doi: 10.1186/s12906-017-2016-5
Figure Lengend Snippet: Effect of JWJTW on insulin signaling in skeletal muscle of diabetic rats. Western blot analysis of phospho-InsR-β Tyr1361 ( a ), phospho-IRS-1 Tyr612 and phospho-IRS-1 Ser307 ( b ), PI3K (p85) ( c ) and GLUT4 ( d ). * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 5)
Article Snippet: The antibodies used in this study were purchased from the following manufacturers: the antibody against pancreatic and duodenal homeobox 1 (PDX-1) was purchased from Chemi-con (USA); against insulin and against insulin receptor (InsR-β) from Abcam (UK); against insulin receptor substrate (IRS)-1 from Upstate (USA); against phospho-insulin receptor substrate (pIRS)-1(Ser307) and pIRS-1(Tyr612),
Techniques: Western Blot