Journal: Cell Reports

Article Title: PIM kinases regulate early human Th17 cell differentiation

doi: 10.1016/j.celrep.2023.113469

Figure Lengend Snippet:

Article Snippet: plasmid CMV6-PIM3 , Origene , Cat# RC212690.

Techniques: Recombinant, Sequencing, Purification, Isolation, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Gene Expression, Reverse Transcription, Staining, CRISPR, Plasmid Preparation, Software

Journal: Cancer medicine

Article Title: Expression and ERG regulation of PIM kinases in prostate cancer.

doi: 10.1002/cam4.3893

Figure Lengend Snippet: FIGURE 2 PIM protein levels are upregulated during PCa progression. IHC staining was performed for FFPE TMA samples of 23 benign prostate, 186 primary PCa, and 45 CRPC samples. Representative IHC figures of whole TMA spots with 5x and 20x enlargement of the refined area are shown from benign prostate, primary PCa and CRPC samples stained with PIM1 (A), PIM2 (B), and PIM3 (C) antibodies. Boxplots were made from IHC staining results by combined Histoscore numbers of nuclear and cytoplasmic staining of the samples. Primary PCa samples were categorized by Gleason scores (GS<7, GS = 7, and GS>7) and PIM1 (D), PIM2 (E), and PIM3 (F) protein expression levels. Error bars display the minimum and maximum values, and the line inside the boxes displays the median in the dataset range. Sample numbers (n) and p-values (p) are marked in the figures. p-values <0.05 (*), p-values <0.01 (**), and p-values <0.001 (***) were considered statistically significant

Article Snippet: Primary antibodies against PIM1 (1:200, ab224772; Abcam), PIM2 (1:50, TA501166; OriGene Technologies Inc.), PIM3 (1:200, TA351349; OriGene), and ERG (1:200, EPR3864; Epitomics, Inc.) were used with the Histofine Simple Stain MAX PO multi; containing both Universal Immunoperoxidase Polymer Anti- Mouse and Anti- Rabbit (Nichirei Biosciences Inc.) secondary antibody according to the manufacturer's instructions.

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Cancer medicine

Article Title: Expression and ERG regulation of PIM kinases in prostate cancer.

doi: 10.1002/cam4.3893

Figure Lengend Snippet: FIGURE 6 ERG binds to the regulatory regions of all PIM genes and regulates their expression. Publicly available ChIP-seq data were used to determine the binding sites for ERG on the PIM1 (A), PIM2 (B), and PIM3 (C) promoter areas. D. qPCR was performed on ERG-silenced (siERG; 25 nM) VCaP cells from which ERG, PIM1, PIM2, and PIM3 transcriptional expression levels were determined after 72 h and compared with cells transfected with control siRNA (NC). TBP was used as a reference gene to normalize the data. E. Western blot analyses of ERG-silenced (siERG; 25 nM) VCaP cells, from which ERG, PIM1, PIM2, and PIM3 protein expression levels were determined after 72 h and compared with cells transfected with control siRNA (NC). Fold changes in protein expression levels were normalized against fibrillarin (FBL) or β-tubulin, which were used as loading controls

Article Snippet: Primary antibodies against PIM1 (1:200, ab224772; Abcam), PIM2 (1:50, TA501166; OriGene Technologies Inc.), PIM3 (1:200, TA351349; OriGene), and ERG (1:200, EPR3864; Epitomics, Inc.) were used with the Histofine Simple Stain MAX PO multi; containing both Universal Immunoperoxidase Polymer Anti- Mouse and Anti- Rabbit (Nichirei Biosciences Inc.) secondary antibody according to the manufacturer's instructions.

Techniques: Expressing, ChIP-sequencing, Binding Assay, Transfection, Control, Western Blot