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Image Search Results
Journal: Oncotarget
Article Title: Control of translational activation by PIM kinase in activated B-cell diffuse large B-cell lymphoma confers sensitivity to inhibition by PIM447
doi: 10.18632/oncotarget.11457
Figure Lengend Snippet: ( A ) IC 50 values of cell lines to PIM447. IC 50 values determined from luminescent viability with the Cell Titer Glo reagent. Indicated cell lines were treated with serial dilutions of PIM447 for 72 hours. Shown is the mean of four replicates ± SEM. ( B ) IC 50 values of ABC and GCB DLBCL cell lines, compared with two-tailed T test. ( C ) mRNA expression of PIM1 , PIM2 and PIM3 in ABC-derived vs. other DLBCL cell lines, compared with two-tailed T test. qRT-PCR was performed on cDNA isolated from each cell line. 2 −ΔΔCT normalized to GAPDH ( D ) IC 50 values calculated from cell viability assays of indicated cell lines, with the top concentration for PIM447 and AZD1208 being 10 μM. Shown is the mean of four replicates ± SEM. ( E ) IC 50 values calculated from cell viability assays of indicated cell lines, with the top concentration for AEB071 being 10 μM and 1 μM for Ibrutinib. Shown is the mean of four replicates ± SEM. ( F ) ABC DLBCL cell lines, grouped based on drug sensitivity, with known somatic mutations.
Article Snippet: Probes used were: Hs0165498_m1 (PIM1), Hs00179139_m1 (PIM2),
Techniques: Two Tailed Test, Expressing, Derivative Assay, Quantitative RT-PCR, Isolation, Concentration Assay