piekny Search Results


rabbit anillin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit anillin
(A) Western blot of HeLa cells transfected with control siRNA (siLuc) or siRNA specific <t>for</t> <t>PRC1.</t> The Western blot was probed with an anti-PRC1 antibody. α-Tubulin is shown as loading control. (B) IF for PLK1, PRC1 and α-tubulin of HeLa cells transfected with siLuc or siPRC1. DNA was visualized using DAPI. Scale bar indicates 5 μm. (C) Representative DIC stills of a live cell imaging experiment of HeLa cells transfected with either siLuc or siPRC1 plus or minus addition of BI2536 (100 nM) at anaphase onset. Scale bar indicates 10 μm. (D) Percentage of cells showing either complete furrow regression, full furrow regression followed by regression, visible but minimal furrow ingression or no furrow ingression (n = 100 cells imaged per condition). **** = p < 0.0001; Chi-squared test for comparison of the indicated conditions, ns = not significant. One representative experiment out of 2 is shown. (E) IF for RhoA and <t>Anillin</t> of HeLa cells in anaphase transfected with the indicated siRNAs and treated plus or minus BI2536 (100 nM). Scale bar indicates 10 μm. (F) Quantification of fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions, ns = not significant.
Rabbit Anillin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anillin/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
rabbit anillin - by Bioz Stars, 2026-04
96/100 stars
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anillinahph domain  (Addgene inc)
93
Addgene inc anillinahph domain
(A) Western blot of HeLa cells transfected with control siRNA (siLuc) or siRNA specific <t>for</t> <t>PRC1.</t> The Western blot was probed with an anti-PRC1 antibody. α-Tubulin is shown as loading control. (B) IF for PLK1, PRC1 and α-tubulin of HeLa cells transfected with siLuc or siPRC1. DNA was visualized using DAPI. Scale bar indicates 5 μm. (C) Representative DIC stills of a live cell imaging experiment of HeLa cells transfected with either siLuc or siPRC1 plus or minus addition of BI2536 (100 nM) at anaphase onset. Scale bar indicates 10 μm. (D) Percentage of cells showing either complete furrow regression, full furrow regression followed by regression, visible but minimal furrow ingression or no furrow ingression (n = 100 cells imaged per condition). **** = p < 0.0001; Chi-squared test for comparison of the indicated conditions, ns = not significant. One representative experiment out of 2 is shown. (E) IF for RhoA and <t>Anillin</t> of HeLa cells in anaphase transfected with the indicated siRNAs and treated plus or minus BI2536 (100 nM). Scale bar indicates 10 μm. (F) Quantification of fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions, ns = not significant.
Anillinahph Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anillinahph domain/product/Addgene inc
Average 93 stars, based on 1 article reviews
anillinahph domain - by Bioz Stars, 2026-04
93/100 stars
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pegfp rhoa biosensor  (Addgene inc)
92
Addgene inc pegfp rhoa biosensor
(A) Western blot of HeLa cells transfected with control siRNA (siLuc) or siRNA specific <t>for</t> <t>PRC1.</t> The Western blot was probed with an anti-PRC1 antibody. α-Tubulin is shown as loading control. (B) IF for PLK1, PRC1 and α-tubulin of HeLa cells transfected with siLuc or siPRC1. DNA was visualized using DAPI. Scale bar indicates 5 μm. (C) Representative DIC stills of a live cell imaging experiment of HeLa cells transfected with either siLuc or siPRC1 plus or minus addition of BI2536 (100 nM) at anaphase onset. Scale bar indicates 10 μm. (D) Percentage of cells showing either complete furrow regression, full furrow regression followed by regression, visible but minimal furrow ingression or no furrow ingression (n = 100 cells imaged per condition). **** = p < 0.0001; Chi-squared test for comparison of the indicated conditions, ns = not significant. One representative experiment out of 2 is shown. (E) IF for RhoA and <t>Anillin</t> of HeLa cells in anaphase transfected with the indicated siRNAs and treated plus or minus BI2536 (100 nM). Scale bar indicates 10 μm. (F) Quantification of fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions, ns = not significant.
Pegfp Rhoa Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp rhoa biosensor/product/Addgene inc
Average 92 stars, based on 1 article reviews
pegfp rhoa biosensor - by Bioz Stars, 2026-04
92/100 stars
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Image Search Results


(A) Western blot of HeLa cells transfected with control siRNA (siLuc) or siRNA specific for PRC1. The Western blot was probed with an anti-PRC1 antibody. α-Tubulin is shown as loading control. (B) IF for PLK1, PRC1 and α-tubulin of HeLa cells transfected with siLuc or siPRC1. DNA was visualized using DAPI. Scale bar indicates 5 μm. (C) Representative DIC stills of a live cell imaging experiment of HeLa cells transfected with either siLuc or siPRC1 plus or minus addition of BI2536 (100 nM) at anaphase onset. Scale bar indicates 10 μm. (D) Percentage of cells showing either complete furrow regression, full furrow regression followed by regression, visible but minimal furrow ingression or no furrow ingression (n = 100 cells imaged per condition). **** = p < 0.0001; Chi-squared test for comparison of the indicated conditions, ns = not significant. One representative experiment out of 2 is shown. (E) IF for RhoA and Anillin of HeLa cells in anaphase transfected with the indicated siRNAs and treated plus or minus BI2536 (100 nM). Scale bar indicates 10 μm. (F) Quantification of fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions, ns = not significant.

Journal: bioRxiv

Article Title: PLK1 plays dual roles in centralspindlin regulation during cytokinesis

doi: 10.1101/317388

Figure Lengend Snippet: (A) Western blot of HeLa cells transfected with control siRNA (siLuc) or siRNA specific for PRC1. The Western blot was probed with an anti-PRC1 antibody. α-Tubulin is shown as loading control. (B) IF for PLK1, PRC1 and α-tubulin of HeLa cells transfected with siLuc or siPRC1. DNA was visualized using DAPI. Scale bar indicates 5 μm. (C) Representative DIC stills of a live cell imaging experiment of HeLa cells transfected with either siLuc or siPRC1 plus or minus addition of BI2536 (100 nM) at anaphase onset. Scale bar indicates 10 μm. (D) Percentage of cells showing either complete furrow regression, full furrow regression followed by regression, visible but minimal furrow ingression or no furrow ingression (n = 100 cells imaged per condition). **** = p < 0.0001; Chi-squared test for comparison of the indicated conditions, ns = not significant. One representative experiment out of 2 is shown. (E) IF for RhoA and Anillin of HeLa cells in anaphase transfected with the indicated siRNAs and treated plus or minus BI2536 (100 nM). Scale bar indicates 10 μm. (F) Quantification of fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions, ns = not significant.

Article Snippet: Primary antibodies used were rabbit KIF20A (Bethyl (ITK) A300-878A), rabbit PRC1 (Santa Cruz, sc-8356), mouse Aurora B (BD Transduction labs, 611083), rabbit Anillin (Piekny & Glotzer 2008) mouse RhoA (Santa Cruz, sc-418), rabbit MKLP1 (Santa Cruz, sc-867), rabbit PLK1 (Santa Cruz, sc-17783), mouse α -tubulin (Thermo Fisher, MA1-80017), goat RACGAP1 (Abcam, ab2270).

Techniques: Western Blot, Transfection, Control, Live Cell Imaging, Comparison, Fluorescence, Standard Deviation

(A) Representative DIC stills of a live cell imaging experiment with HeLa cells transfected with the indicated siRNAs and treated plus/minus the Aurora B inhibitor ZM447439 (2 μM) prior to anaphase onset. Scale bar indicates 10 μm. (B) Cells were treated and imaged as in (A), and the percentage of cells showing either stable furrow ingression, furrow ingression followed by furrow regression, minimal furrow ingression, no furrow ingression was scored. The number of cells analyzed per condition is indicated (n). One representative experiment out of 2 is shown. **** = p < 0.0001; Chi squared test for comparison of the indicated conditions, ns = not significant. (C) IF for Anillin and RhoA. DNA was visualized using DAPI. Scale bar = 5 μm. (D) Quantification of the fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions.

Journal: bioRxiv

Article Title: PLK1 plays dual roles in centralspindlin regulation during cytokinesis

doi: 10.1101/317388

Figure Lengend Snippet: (A) Representative DIC stills of a live cell imaging experiment with HeLa cells transfected with the indicated siRNAs and treated plus/minus the Aurora B inhibitor ZM447439 (2 μM) prior to anaphase onset. Scale bar indicates 10 μm. (B) Cells were treated and imaged as in (A), and the percentage of cells showing either stable furrow ingression, furrow ingression followed by furrow regression, minimal furrow ingression, no furrow ingression was scored. The number of cells analyzed per condition is indicated (n). One representative experiment out of 2 is shown. **** = p < 0.0001; Chi squared test for comparison of the indicated conditions, ns = not significant. (C) IF for Anillin and RhoA. DNA was visualized using DAPI. Scale bar = 5 μm. (D) Quantification of the fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the standard deviation (SD). **** = p < 0.0001; Student’s t-test for comparison of the indicated conditions.

Article Snippet: Primary antibodies used were rabbit KIF20A (Bethyl (ITK) A300-878A), rabbit PRC1 (Santa Cruz, sc-8356), mouse Aurora B (BD Transduction labs, 611083), rabbit Anillin (Piekny & Glotzer 2008) mouse RhoA (Santa Cruz, sc-418), rabbit MKLP1 (Santa Cruz, sc-867), rabbit PLK1 (Santa Cruz, sc-17783), mouse α -tubulin (Thermo Fisher, MA1-80017), goat RACGAP1 (Abcam, ab2270).

Techniques: Live Cell Imaging, Transfection, Comparison, Fluorescence, Standard Deviation