Journal: Cell reports

Article Title: Epinephrine inhibits PI3Kα via the Hippo kinases

doi: 10.1016/j.celrep.2023.113535

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PIK3CG was purchased from Thermo Fisher , SignalChem , P27–10CG.

Techniques: Recombinant, Fluorescence, Isolation, Double Knockout, Mutagenesis, Plasmid Preparation, Software

Journal: Food Science and Human Wellness

Article Title: Sucrose-free hawthorn leathers formulated with fructooligosaccharides and xylooligosaccharides ameliorate high-fat diet induced inflammation, glucose and lipid metabolism in liver of mice

doi: 10.1016/j.fshw.2022.03.033

Figure Lengend Snippet: Fig. 4 Effect of hawthorn leather treatment on expression levels of inflammatory cytokines and NF-κB signaling pathway in the liver tissue of mice. mRNA expression level of (A) IL-1β, (B) Nos2, (C) Cox2, (D) TLR4, (E) MyD88, and (F) NF-κB; (G) Images of western blotting; Protein expression ratio of (H) MyD88 to GAPDH and (I) NF-κB to GAPDH. Data are expressed as mean ± SD (8 mice/group). Values of each group with different letters over the bars are significantly different (P < 0.05) by analysis of one-way ANOVA followed by Tukey’s multiple range test.

Article Snippet: Then, the membrane was incubated with specific primary antibody (1 : 1 000) against MyD88 (CST, cat. number 4283S), NF-κB (p65) (CST, cat. number 8242S), PI3K (p110β) (Proteintech, cat. number 20584-1-AP), AKT (Proteintech, cat. number 10176-2-AP), Thr308 phosphorylated AKT (p-AKT) (CST, cat. number 13038T), mTOR (Proteintech, cat. number 20657-1-AP), Ser2448 phosphorylated mTOR (p-mTOR) (CST, cat. number 5536T), PPAR-γ (CST, cat. number 2435T), FASN (CST, cat. number 3180T), and HSL (CST, cat. number 18381T) overnight at 4 °C, respectively.

Techniques: Expressing, Western Blot

Journal: Biological & pharmaceutical bulletin

Article Title: Astragalus Polysaccharide Alleviates Cognitive Decline in D-Galactose-Induced Aging.

doi: 10.1248/bpb.b24-00524

Figure Lengend Snippet: Fig. 9. Effects of APS on the Expression of p-PI3K, p-AKT, NAMPT, SIRT1 and TERT in the Hippocampus of Aging Rats

Article Snippet: The membrane was blocked with 5% nonfat milk at room temperature for 1 h. The membranes were subsequently incubated with the following primary antibodies at 4°C overnight: phosphatidylinositol 3-kinase (PI3K) (1 : 5000, 60225-1-Ig, Proteintech, California, U.S.A.), p-PI3K (1 : 1000, ab182651, Abcam, Cambridge, U.K.), Akt (1 : 1000, 4691, Cell Signaling Technology, MA, U.S.A.), p-Akt (1 : 2000, 4060, Cell Signaling Technology), nicotinamide phosphoribosyl transferase (NAMPT) (1 : 1500, PA585535, Thermo Fisher Scientific, Waltham, MA, U.S.A.), SIRT1 (1 : 1000, PA5-116530, Thermo Fisher Scientific), TERT (1 : 1000, ab16502, Abcam, Cambridge, U.K.), p53 (1 : 2000, PA5-88098, Thermo Fisher Scientific), p21 (1 : 200, 14-6715-81, Thermo Fisher Scientific), and the membrane was washed with TBST and incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit/mouse IgG antibody (1 : 25000/1 : 10000, Servicebio Technology Co., Ltd., China) at room temperature for 1 h. The protein bands were detected via an enhanced chemiluminescence (ECL) Western blotting detection kit (SW2020, Solarbio, China).

Techniques: Expressing

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21.

doi: 10.1080/21691401.2020.1725533

Figure Lengend Snippet: Figure 6. Silence of MEG3 activates PI3K/AKT pathway while deactivates NF-jB pathway via targeting miR-21. PC12 cells were co-transfected with si-MEG3/si-NC and miR-21 inhibitor/NC inhibitor, and then incubated in hypoxia for 24 h. The cells without transfection in normoxic condition served as control (CTRL). The expres- sion of proteins involved in (A,B) PI3K/AKT and (C,D) NF-jB pathways was tested by western blot. n ¼ 3.p < .05 represents a comparison of marked groups.

Article Snippet: Proteins were carried out by using primary antibodies specific for CyclinD1 (orb77046), p53 (orb178524), p21 (orb38089), Bcl-2 (orb378569), Bax (orb378566), pro-caspase-3 (orb13278), cleaved-caspase-3 (orb310032), PTEN (orb39110), PI3K (orb395443), p-PI3K (orb106105), AKT (orb29949), p-AKT (orb344403), IjBa (orb13487), p-IjBa (orb338946), p65 (orb344389), p-p65 (orb106211), and b-actin (orb378579, all from Biorbyt, San Francisco, CA).

Techniques: Transfection, Incubation, Control, Western Blot, Comparison

Journal: Cell Reports Medicine

Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B

doi: 10.1016/j.xcrm.2023.101285

Figure Lengend Snippet:

Article Snippet: Plasmid: pBabe puro pI3K p110 CAAX , Addgene , Cat# 13339.

Techniques: Western Blot, Immunohistochemistry, Immunoprecipitation, Microarray, Recombinant, Transfection, Plasmid Preparation, Membrane, Staining, Flow Cytometry, Viability Assay, Chromatin Immunoprecipitation, Phospho-proteomics, shRNA, Sequencing, Knockdown, Binding Assay, Control, Software, Expressing, Gene Expression

Journal: Biomarker Research

Article Title: Phosphatidylinositol 3-kinase-δ (PI3K-δ) is a potential therapeutic target in adult T-cell leukemia-lymphoma

doi: 10.1186/s40364-018-0138-7

Figure Lengend Snippet: PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon). Whole-cell lysates were extracted from isolated CD4 + cells using Dynabeads (Invitrogen) from the PBMCs of healthy donors ( n = 4) and ATL patients ( n = 8). b PI3K-δ expression was identified by immunoblotting using the PBMCs of HTLV-1 uninfected healthy donors ( n = 4), and ATL patients in whom the ATL clone constituted more than 75% of PBMCs ( n = 11)

Article Snippet: Fig. 1 PI3K activity and expression of PI3K-δ in ATL cells. a The activity of PI3K was quantified by PI3-Kinase Activity ELISA kit (Echelon).

Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Isolation, Western Blot