phosphorylation Search Results


variant  (Sino Biological)
94
Sino Biological variant
Variant, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gsk3β phosphorylated tau  (Sino Biological)
92
Sino Biological gsk3β phosphorylated tau
Gsk3β Phosphorylated Tau, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fmoc ser po obzl oh oh pser  (Chem Impex International)
95
Chem Impex International fmoc ser po obzl oh oh pser
Fmoc Ser Po Obzl Oh Oh Pser, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho marcks ser159 163 polyclonal antibody  (Proteintech)
93
Proteintech phospho marcks ser159 163 polyclonal antibody
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cas  (Chem Impex International)
95
Chem Impex International cas
Cas, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit p chk2 t68  (Rockland Immunochemicals)
96
Rockland Immunochemicals rabbit p chk2 t68
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phosphorylated neurofilament h human elisa kit  (BioVendor Instruments)
94
BioVendor Instruments phosphorylated neurofilament h human elisa kit
Phosphorylated Neurofilament H Human Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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coenzyme a  (Chem Impex International)
95
Chem Impex International coenzyme a
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nicotinamide adenine dinucleotide nad  (Chem Impex International)
95
Chem Impex International nicotinamide adenine dinucleotide nad
Nicotinamide Adenine Dinucleotide Nad, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho s6k1  (Boster Bio)
90
Boster Bio phospho s6k1
(A) Cytotoxicity of SK-Hep1 cells treated with IR (2Gy) alone or combined with PKI-587 (0.1 μM) at 0, 12, 24, 36, and 48 h. (B) Clonogenic survival assays of SK-Hep1 cells treated with PKI-587 (0.1μM) followed by irradiation in a range of radiation doses. (C and F) Representative images of the colony formation revealing the proliferation of SK-Kep1 cells treated with IR (2 Gy) alone or combined with PKI-587 (0.1 μM) for 24 h. The percentage of colony formation was calculated. (D and E) The level of <t>p-S6K1</t> and p70S6K proteins in SK-Hep1 cells after treatment with IR (2Gy) alone or combined with PKI-587 (0.1 μM) for 24 h determined by western blot assay. The semiquantitative data were represented as p-S6K1/p70S6K. The data are mean ± SD, n = 3. ** P <0.01, *** P <0.001 versus IR group. IR, ionizing radiation (6 MV-X ray).
Phospho S6k1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cmlck  (Proteintech)
85
Proteintech cmlck
(A) Cytotoxicity of SK-Hep1 cells treated with IR (2Gy) alone or combined with PKI-587 (0.1 μM) at 0, 12, 24, 36, and 48 h. (B) Clonogenic survival assays of SK-Hep1 cells treated with PKI-587 (0.1μM) followed by irradiation in a range of radiation doses. (C and F) Representative images of the colony formation revealing the proliferation of SK-Kep1 cells treated with IR (2 Gy) alone or combined with PKI-587 (0.1 μM) for 24 h. The percentage of colony formation was calculated. (D and E) The level of <t>p-S6K1</t> and p70S6K proteins in SK-Hep1 cells after treatment with IR (2Gy) alone or combined with PKI-587 (0.1 μM) for 24 h determined by western blot assay. The semiquantitative data were represented as p-S6K1/p70S6K. The data are mean ± SD, n = 3. ** P <0.01, *** P <0.001 versus IR group. IR, ionizing radiation (6 MV-X ray).
Cmlck, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho threonine derivative fmoc thr hpo 3 bzl oh  (Chem Impex International)
95
Chem Impex International phospho threonine derivative fmoc thr hpo 3 bzl oh
(A) Cytotoxicity of SK-Hep1 cells treated with IR (2Gy) alone or combined with PKI-587 (0.1 μM) at 0, 12, 24, 36, and 48 h. (B) Clonogenic survival assays of SK-Hep1 cells treated with PKI-587 (0.1μM) followed by irradiation in a range of radiation doses. (C and F) Representative images of the colony formation revealing the proliferation of SK-Kep1 cells treated with IR (2 Gy) alone or combined with PKI-587 (0.1 μM) for 24 h. The percentage of colony formation was calculated. (D and E) The level of <t>p-S6K1</t> and p70S6K proteins in SK-Hep1 cells after treatment with IR (2Gy) alone or combined with PKI-587 (0.1 μM) for 24 h determined by western blot assay. The semiquantitative data were represented as p-S6K1/p70S6K. The data are mean ± SD, n = 3. ** P <0.01, *** P <0.001 versus IR group. IR, ionizing radiation (6 MV-X ray).
Phospho Threonine Derivative Fmoc Thr Hpo 3 Bzl Oh, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Cytotoxicity of SK-Hep1 cells treated with IR (2Gy) alone or combined with PKI-587 (0.1 μM) at 0, 12, 24, 36, and 48 h. (B) Clonogenic survival assays of SK-Hep1 cells treated with PKI-587 (0.1μM) followed by irradiation in a range of radiation doses. (C and F) Representative images of the colony formation revealing the proliferation of SK-Kep1 cells treated with IR (2 Gy) alone or combined with PKI-587 (0.1 μM) for 24 h. The percentage of colony formation was calculated. (D and E) The level of p-S6K1 and p70S6K proteins in SK-Hep1 cells after treatment with IR (2Gy) alone or combined with PKI-587 (0.1 μM) for 24 h determined by western blot assay. The semiquantitative data were represented as p-S6K1/p70S6K. The data are mean ± SD, n = 3. ** P <0.01, *** P <0.001 versus IR group. IR, ionizing radiation (6 MV-X ray).

Journal: PLoS ONE

Article Title: PKI-587 enhances radiosensitization of hepatocellular carcinoma by inhibiting the PI3K/AKT/mTOR pathways and DNA damage repair

doi: 10.1371/journal.pone.0258817

Figure Lengend Snippet: (A) Cytotoxicity of SK-Hep1 cells treated with IR (2Gy) alone or combined with PKI-587 (0.1 μM) at 0, 12, 24, 36, and 48 h. (B) Clonogenic survival assays of SK-Hep1 cells treated with PKI-587 (0.1μM) followed by irradiation in a range of radiation doses. (C and F) Representative images of the colony formation revealing the proliferation of SK-Kep1 cells treated with IR (2 Gy) alone or combined with PKI-587 (0.1 μM) for 24 h. The percentage of colony formation was calculated. (D and E) The level of p-S6K1 and p70S6K proteins in SK-Hep1 cells after treatment with IR (2Gy) alone or combined with PKI-587 (0.1 μM) for 24 h determined by western blot assay. The semiquantitative data were represented as p-S6K1/p70S6K. The data are mean ± SD, n = 3. ** P <0.01, *** P <0.001 versus IR group. IR, ionizing radiation (6 MV-X ray).

Article Snippet: Antibodies to p70S6K, phospho-S6K1 (T421 + S424), eIF4EBP1 and phospho-eIF4EBP1 were purchased from Boster Biological Technology, China. γ-H2AX was obtained from Biolegend Biological Technology, USA. β-actin was obtained from Biosharp Life Science, China.

Techniques: Irradiation, Western Blot