Journal: Communications Biology

Article Title: Bmp8a deletion leads to obesity through regulation of lipid metabolism and adipocyte differentiation

doi: 10.1038/s42003-023-05194-2

Figure Lengend Snippet: a The serum TG, TC and GLU level in WT and bmp8a -/- zebrafish ( n = 6). b , c The qPCR analysis of genes related to fatty acid metabolism in the WT and bmp8a -/- zebrafish liver ( b , n = 3) or adipose tissue ( c , n = 3). d , e The bssl1 ( d ) and bssl2 ( e ) gene expression analysis in different zebrafish tissues ( n = 3). f , g The qPCR analysis of bssl1 and bssl2 mRNA level in the liver ( f , n = 3) and intestine ( g , n = 3) from WT or bmp8a -/- zebrafish. h – j Validation and quantification of p-AMPK, p-ACC, Ucp1, and Pgc-1α expression in adipose tissue ( i ) and liver ( j ) from WT or bmp8a -/- zebrafish. Protein expression levels were quantified using ImageJ software and normalized to total protein or β-actin ( n = 3). k – m Validation and quantification of p-p38 MAPK, p-AMPK, p-ACC, Ucp1, and Pgc-1α expression after overexpression of zebrafish bmp8a in ZFL cells. The cells were collected at 36 h ( I ) and 48 h ( m ) post-transfection for Immunoblot analysis. Protein expression levels were quantified using ImageJ software and normalized to total protein or β-actin ( n = 3). n Schematic overview. Data were representative of at least three independent experiments. Data were analyzed by two-tailed Student’s t -test and presented as mean ± SD (** p < 0.01, *** p < 0.001).

Article Snippet: Antibodies from ZenBio: p-AMPK (1:1000, #R26252), AMPK (1:1000, #380431), PGC-1α (1:1000, #381615).

Techniques: Expressing, Software, Over Expression, Transfection, Western Blot, Two Tailed Test