Journal: Medical physics

Article Title: Development of a storage phosphor imaging system for proton pencil beam spot profile determination

doi: 10.1002/mp.15139

Figure Lengend Snippet: (a): Laboratory optical scanner used for two-dimensional readout of storage phosphor dosimeters. Dosimeters were mounted on a vertical table connected to two orthogonal sliders. Light from a 2 mW, 594 nm He-Ne laser was focused upon the table surface. The intensity of the photostimulated luminescence light was collected by a Spectralon-coated 5.1 cm diameter integrating sphere and measured by the photomultiplier tube. (b): Dosimeter samples of sizes 5 cm × 5 cm were mounted upon acrylic mounting plates of sizes 7 cm × 5 cm for ease of handling. These plates were taped to the vertical sliding table (Figure 1a) during readout. Alignment guides were used to ensure consistent dosimeter mounting positions. Table motion resulted in the raster scanning pattern of the laser as shown in red. Active readout occurred during translations of the laser spot along the x-axis. A y-axis displacement of Δy (to be determined in the subsequent section) was applied between line scans [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Square 5 × 5 cm 2 dosimeter samples were cut from a BaFBrI:Eu 2+ -based storage phosphor dosimeter panel (Model ST-VI, Fujifilm).

Techniques:

Journal: Medical physics

Article Title: Development of a storage phosphor imaging system for proton pencil beam spot profile determination

doi: 10.1002/mp.15139

Figure Lengend Snippet: (a): A two-dimensional (2D) dosimetric image of a 1 MU proton spot at maximum energy (227.1 MeV) as measured by a commercial scintillation detector (Lynx PT), and (b): Similar dosimetric images measured using BaFBr0.85I0.15:Eu2+ storage phosphors, where 1 MU = 1 Gy under reference conditions, that is, 41 × 41 spots at 227.1 MeV proton energy equally spaced over 10 × 10 cm2 field size at 5.0 cm depth at isocenter. (c): Plots of 1 MU crossline profiles of both the Lynx PT scintillation detector and BaFBr0.85I0.15:Eu2+ storage phosphor dosimeters through the center of each 2D spot profile. Data were normalized to their peak crossline values. (d): Similar plots of the 1 MU inline profiles. Data were normalized to their peak inline values [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Square 5 × 5 cm 2 dosimeter samples were cut from a BaFBrI:Eu 2+ -based storage phosphor dosimeter panel (Model ST-VI, Fujifilm).

Techniques:

Journal: Medical physics

Article Title: Development of a storage phosphor imaging system for proton pencil beam spot profile determination

doi: 10.1002/mp.15139

Figure Lengend Snippet: (a): Plots of the crossline profiles of a proton spot at maximum energy (227.1 MeV) as measured by BaFBr0.85I0.15:Eu2+ storage phosphor dosimeters at various proton doses. Data were normalized to each individual crossline profile to overlay the profiles for visual comparison purposes. (b): Similar crossline profiles at various proton doses with normalization done at the peak value of the 10 MU level. BaFBr0.85I0.15:Eu2+ demonstrated an approximate linearity to increasing doses across a dynamic range spanning from 1 to 50 MU. (c): Similar inline profiles of a proton spot at maximum energy at various doses with data normalization at the peaks of each individual inline profile. (d): Similar inline profiles at various proton doses with normalization performed at the peak value of the 10 MU level [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Square 5 × 5 cm 2 dosimeter samples were cut from a BaFBrI:Eu 2+ -based storage phosphor dosimeter panel (Model ST-VI, Fujifilm).

Techniques:

Journal: Medical physics

Article Title: Development of a storage phosphor imaging system for proton pencil beam spot profile determination

doi: 10.1002/mp.15139

Figure Lengend Snippet: Gaussian fitting parameters of two-dimensional (2D) proton spot profile measurements using a Lynx PT scintillation detector and BaFBr 0.85 I 0.15 :Eu 2+ storage phosphor dosimeters as a function of proton dose delivered (MU)

Article Snippet: Square 5 × 5 cm 2 dosimeter samples were cut from a BaFBrI:Eu 2+ -based storage phosphor dosimeter panel (Model ST-VI, Fujifilm).

Techniques:

Journal: Medical physics

Article Title: Development of a storage phosphor imaging system for proton pencil beam spot profile determination

doi: 10.1002/mp.15139

Figure Lengend Snippet: Gaussian fitting parameters of two-dimensional (2D) proton spot measurements as measured by BaFBr 0.85 I 0.15 :Eu 2+ storage phosphor dosimeters as a function of incident proton energy (MeV)

Article Snippet: Square 5 × 5 cm 2 dosimeter samples were cut from a BaFBrI:Eu 2+ -based storage phosphor dosimeter panel (Model ST-VI, Fujifilm).

Techniques:

Journal: RNA Biology

Article Title: Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation

doi: 10.4161/rna.8.5.16022

Figure Lengend Snippet: YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.

Article Snippet: [ 14 C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.).

Techniques: Mutagenesis, Luciferase, Control, Labeling, SDS Page, Autoradiography, Radioactivity