Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Use of Inhibitors in the Study of MAP Kinases

doi: 10.1007/978-1-60761-795-2_6

Figure Lengend Snippet: List of antibodies for the phosphorylated and phosphorylation-independent (total) forms of MAP kinases and phosphorylated forms of MAP kinase substrate proteins

Article Snippet: , Stat-1 (S727) , CST (9177).

Techniques: Phospho-proteomics

Journal: Nature immunology

Article Title: TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity

doi: 10.1038/ni.3143

Figure Lengend Snippet: TLR7-induced phosphorylation of MAPKs and STAT1 is regulated by TREML4. ( a-d ) Phosphoflow cytometry analysis of p44p42 ( a ), p38 ( b ), pSTAT1(S727) ( c ) and pSTAT1(Y701) ( d ) in CD11c + BMDCs isolated from Treml4 +/+ and Treml4 –/– mice treated with 100 ng/mL LPS, 10 μg/mL GRD, or 10 μg/mL CpG-DNA for 30 min. Histograms are representative of one experiment of 3 independent experiments performed. Graphs represent the mean fluorescent intensity (MFI). * P <0.01 (Student's t -test; error bars, mean and s.d.).

Article Snippet: Cells were washed twice to remove methanol and stained with fluorescently labeled antibodies CD11c-APC, p42/44-Alexa488 (Y202/204; Cell Signaling), p38MAPK-PE (T180/Y182; Cell Signaling), pSTAT1 (S727; Cell Signaling clone D3B7), pSTAT1 (Y701; Cell Signaling clone 58D6).

Techniques: Phospho-proteomics, Cytometry, Isolation