Journal: Brain research bulletin

Article Title: Effects of Interleukin-19 overexpression in the medial prefrontal cortex on anxiety-related behaviors, BDNF expression and p38/JNK/ERK pathways.

doi: 10.1016/j.brainresbull.2024.110952

Figure Lengend Snippet: Fig. 7. Western blot images and quantitative data showing protein and phosphorylation alterations of p38, JNK, and Erk in the mPFC of AAV-CaMKII-GFP (NC) and AAV-CaMKII-IL-19-GFP (IL-19) mice. (A) Representative western blot images. Quantitative data showing (B) protein levels and (C) phosphorylated protein levels of p38, JNK, and Erk. n = 5 per group. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group.

Article Snippet: The membrane was blocked at room temperature for 2 h using 5 % bovine serum albumin (BSA) in 1×TBST buffer (150 mM NaCl, 20 mM Tris-HCl [pH 7.4], and 0.1 % Tween-20) and incubated at 4 ◦C overnight with the following primary antibodies diluted in prepared blocking buffer: anti-IL-19 (1:500, ab154187, Abcam, Cambridge, UK), anti-BDNF (1:1000, ab108319, Abcam), anti-PSD95 (1:500, sc-32290, Santa Cruz Biotechnology, CA, USA), anti-IL-20Rα (1:500, bs-2619R, Bioss antibody, Woburn, MA, USA), anti-phospho-p38 MAPK(1:1000, #9215, Cell Signaling Technology, Danvers, MA, USA), anti-p38 MAPK (1:1000, #9212, Cell Signaling), anti-phospho-JNK (1:1000, #9251, Cell Signaling), anti-JNK (1:1000, #9252, Cell Signaling), anti-phospho-Erk1/2 (1:1000, #4370, Cell Signaling), anti-Erk1/2 (1:1000, #9102, Cell Signaling), or anti-β-actin (1:2000, #3700, Cell Signaling) antibody.

Techniques: Western Blot, Phospho-proteomics, Control

Journal: Cell

Article Title: Ribosome collisions trigger general stress responses to regulate cell fate

doi: 10.1016/j.cell.2020.06.006

Figure Lengend Snippet: (A) Immunoblots for phosphorylation of eIF2α and mTOR in MCF10A cells treated with ANS (0.1–100 mg/L, 0.5 h). Total eIF2α and β-actin as loading controls. (B) eIF2α phosphorylation induced by intermediate doses of ANS (0.5 mg/L, 0.5 h) in MCF10A cells pretreated with GCN2 inhibitor (A-92), PERK inhibitor (GSK 2606414), or p38 inhibitor (BIRB 796). (C) eIF2α phosphorylation induced by ANS in WT or ZAK KO MCF10A cells. (D) eIF2α phosphorylation in WT, ZAK KO, or ZAK KO MCF10A complemented with ZAKα, ZAKα -K45M, or ZAKβ under ANS treatment (0.5 mg/L, 0.5 h). (E) Polysome profiles from DSP-crosslinked WT and ZAK KO MCF10A cells. Fractions were analyzed by immunoblotting with indicated antibodies.

Article Snippet: Antibodies for phospho-p38 (Thr180/Tyr182, 9211), p38 (9212), phospho-JNK (Thr183/Tyr185, 4668), β-actin (51255), phospho-mTOR (Ser2448, 2971) and eIF2α (9722S) were from Cell Signaling Technology.

Techniques: Western Blot, Phospho-proteomics

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Hawthorne leaf flavonoids prevent oxidative stress injury of renal tissues in rats with diabetic kidney disease by regulating the p38 MAPK signaling pathway

doi:

Figure Lengend Snippet: Immunochemical analysis of the (A) p38MAPK (B) and p-p38MAPK expression in the kidney of different groups (200×). Quantitative analysis of (C) p38MAPK and (D) p-p38MAPK expression in the kidney of different groups. The expression of p38MPAK and p-p38MAPK was significantly higher in the DKD group than in the HLF and IRB groups.

Article Snippet: Rabbit anti-rat p38MAPK and p-p38MAPK polyclonal antibodies were purchased from Boster (Beijing, China).

Techniques: Expressing