Journal: Chemistry & biology

Article Title: Selecting agonists from single cells infected with combinatorial antibody libraries.

doi: 10.1016/j.chembiol.2013.04.012

Figure Lengend Snippet: Figure 5. Signal Transduction by Agonist Antibodies JAK2 was purified from cell lysates using affinity to anti-JAK2 agarose and its phosphorylation was detected with western blotting using anti- phosphotyrosine antibodies. Phosphorylation of STAT3, STAT5, Akt, and MAPK induced by agonist antibodies or rhTPO stimulation was detected with direct western blotting of cell lysates. See also Figure S6.

Article Snippet: The immunoprecipitates were analyzed with western blotting using anti-phospho-JAK2 (Tyr1007/1008) (C80C3) antibody (Cell Signaling Technology, catalog no. 3776) and anti-total JAK2 antibody (C-14) (Santa Cruz Biotechnology, catalog no. sc-34479).

Techniques: Transduction, Phospho-proteomics, Western Blot

Journal: Scientific Reports

Article Title: Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis

doi: 10.1038/s41598-023-32093-7

Figure Lengend Snippet: PTX inhibit Cl 2 -induced ferroptosis. ( A , B ) The expression levels of MDA, GSH/GSSG in mice serum treated with Cl 2 , n = 8. ( C , D ) Protein levels of GPX4 and SLC7A11 in mice lung and serum treated with Cl 2 , n = 8. ( E ) GPX4 and SLC7A11 protein levels were determined by western blot analysis, and standardized to the expression of β-actin. Original scans of whole blots are shown in Supplementary information 5. ( F , G ) Immunofluorescence images were used to detect the expression of GPX4 in epithelial and endothelial cells, scale bars 50 μm, n = 6. ( H , I ) Immunofluorescence images were used to detect the expression of SLC7A11 in epithelial and endothelial cells, scale bars 50 μm, n = 6. Epithelial cell: CK8 (green), endothelial cells: Pecam1 (green), GPX4 (red), SLC7A11 (red), cell nuclear (blue). The data was shown as mean ± SEM, * P < 0.05, ** p < 0.01, p < 0.001, **** p < 0.0001, n = 8.

Article Snippet: WB was performed using the following primary antibodies: SLC7A11(orb100617, biorbyt); GPX4 (orb135590, biorbyt); β-Actin (orb625112, biorbyt).

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) SPRC and NaHS markedly decreased serum IL-6 levels induced by LPS (n = 5). (B-D) Hepatic Il-6 , Tnfa mRNA levels and JAK2/STAT3 phosphorylation were induced by LPS, and suppressed by SPRC and NaHS. Moreover, PAG diminished the effects of SPRC (n = 5). (E-F) Densitometry analysis of Fig 2D. (G-H) Hepatic mRNA levels of Socs3 and Saa2 , two target genes of STAT3 (n = 5). Representative immunoblots are presented. Whole uncropped images of Fig 2D are shown in . Data are presented as the mean ± SEM. ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the LPS group; @ p < 0.05, @@ p < 0.01, @@@ p < 0.001.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) Serum IL-6 content was not significantly changed by SPRC (n = 7). (B) Hepatic Il-6 mRNA levels were suppressed by SPRC, though only 2-fold change was observed in the model group (n = 7). (C) SPRC successfully ameliorated hepatic JAK2/STAT3 phosphorylation in the turpentine model. (D-E) Densitometry analysis of . Representative immunoblots are presented. Whole uncropped images of Fig 4C are shown in . Data are presented as the mean ± SEM. # p < 0.05, ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the model group.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot