Journal: Cancers

Article Title: TP-0903 Is Active in Preclinical Models of Acute Myeloid Leukemia with TP53 Mutation/Deletion.

doi: 10.3390/cancers15010029

Figure Lengend Snippet: Figure 3. TP-0903 induces DNA damage response in TP53 mutant AML cell lines. (A) MV4-11 (R248W), (B) HL-60, and (C) Kasumi-1 cells were treated with DMSO or TP-0903 at indicated concentrations for 4 h. Immunoblotting was performed to determine the expression of pChk1 (Ser345), pChk2 (Thr68), and pH2AX (Ser139/Tyr142). Vinculin served as the loading control for pChk1/Chk1 and pChk2/Chk2 blots. GAPDH served as the loading control for pH2AX/H2AX blots. Immunoblots were quantified against respective loading controls (vinculin or GAPDH) using ImageJ. Blots are representative of 2–3 independent experiments.

Article Snippet: Antibodies against p53 (48818; clone DO-7), p21 (2947; clone 12D1), pH2AX (Ser 139) (80312; clone D7T2V), pH2AX (Ser139/Tyr142) (5438; clone N/A), H2AX (7631; clone D17A3), pAURKA(Thr288)/B(Thr232)/C(Thr198) (2914; clone D13A11), AURKA (91590; clone D3V7T), AURKB (3094; clone N/A), pChk1 (Ser345) (2348; clone 133D3), pChk2 (Thr68) (2197; clone C13C1), Chk1 (2360; clone 2G1D5), Chk2 (6334; clone D9C6), vinculin (13901; clone E1E9V), GAPDH (5174S; clone D16H11), and HRP-conjugate secondary antirabbit (7074) were obtained from Cell Signaling Technology (CST) (Danvers, MA, USA).

Techniques: Mutagenesis, Western Blot, Expressing, Control

Journal: Journal of Ayurveda and Integrative Medicine

Article Title: A herbal formulation inhibits growth and survival of lung cancer cells through DNA damage and apoptosis - in vitro and in vivo studies

doi: 10.1016/j.jaim.2025.101313

Figure Lengend Snippet: Effect of SL on DNA damage and response (DDR) proteins in A549 cells. A549 cells were treated with SL for 48 h and stained with acridine orange/ethidium bromide (AO/EB) and observed under the fluorescent microscope. (A) Images of A549 stained with AO/ET at 200x magnification. (B) Percentage of apoptotic cells. (C) SL-induced DNA fragmentation in A549 cells. (D) SL affected DNA damage response and repair-related proteins namely, Chk1, Chk2, and Rad51 in A549 cells. Data points are the means ± s.e.m. of three independent experiments. Bars, s.e.m, ∗∗∗P < 0.001. SL, Safoof Lajward . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibodies for Bcl-2, #2872; Bax, #2772; Chk1, #2360; Chk2, #3440; Rad51, #82263; PCNA, #2586; Ki-67, #9449; Cleaved caspase3, # 9664; Cleaved PARP, #5625; and Beta-actin, #A3853, and secondary antibodies, anti-mouse, #7076 and anti-rabbit, #7074P2, were acquired from Cell Signaling Technology (CST), USA.

Techniques: Staining, Microscopy

Journal: Journal of Ayurveda and Integrative Medicine

Article Title: A herbal formulation inhibits growth and survival of lung cancer cells through DNA damage and apoptosis - in vitro and in vivo studies

doi: 10.1016/j.jaim.2025.101313

Figure Lengend Snippet: Effect of SL on molecular markers of proliferation, apoptosis, and DDR proteins in tumor tissue . (A) H&E and immunohistochemical (IHC) profiling of A549 tumor tissues from nude mice showing decreased proliferation, Ki-67 positivity, and increased apoptosis, cleaved caspase 3, and cleaved PARP positivity in the SL treated group. H&E images at 200x and IHC images at 400x magnification, scale bar is 50 μm. (B–D) Quantification of Ki-67, cleaved caspase 3, and cleaved PARP positive cells. (E) Western bot analysis of SL treated tumors on apoptosis, DNA damage pathway, and cell survival proteins (PCNA, Bax, Bcl-2, Chk1, Chk2, and Rad51) in xenograft tumor tissues. (F–J) Quantification of a Western blot for the protein expression levels of PCNA, Chk1, Chk2, Rad51, and Bax/Bcl-2 ratio. T1, T2, and T3 are different tumor samples from the control and treated groups. ∗P < 0.05, ∗∗∗P < 0.001. SL, Safoof Lajward .

Article Snippet: Primary antibodies for Bcl-2, #2872; Bax, #2772; Chk1, #2360; Chk2, #3440; Rad51, #82263; PCNA, #2586; Ki-67, #9449; Cleaved caspase3, # 9664; Cleaved PARP, #5625; and Beta-actin, #A3853, and secondary antibodies, anti-mouse, #7076 and anti-rabbit, #7074P2, were acquired from Cell Signaling Technology (CST), USA.

Techniques: Immunohistochemical staining, Western Blot, Expressing, Control

Journal: International Journal of Medical Sciences

Article Title: Cedrus atlantica Extract Suppress Glioblastoma Growth through Promotion of Genotoxicity and Apoptosis: In Vitro and In Vivo Studies

doi: 10.7150/ijms.54468

Figure Lengend Snippet: Primary antibodies information.

Article Snippet: p-Chk2 , Biorbyt Ltd. , Orb5862 , Rabbit , WB: 1/1000.

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Hepatitis B virus X stimulates redox signaling through activation of ataxia telangiectasia mutated kinase

doi:

Figure Lengend Snippet: Phosphorylation of ATM is oxidant-dependent. (A and B) Cells were treated with H2O2 (100 μM) for 30 min or with antioxidant NAC (5 mM) for 2 h. Levels of phospho-ATM (p-ATM) and phospho-Chk2 (p-Chk2) were determined by western blotting. Columns represent relative band intensities normalized against β-actin expressed as fold changes relative to control plasmid-transfected cells (a*, p<0.05 vs. mock plasmid transfected cells; b*, p<0.05 vs. pHBX-transfected cells). (C) Immunohistochemical staining of p-ATM and p-Chk2 in livers. Arrows indicate p-ATM- or p-Chk2-positive non-parenchymal cells (magnification x 40). Columns show labeling indices in hepatocytes. Data are expressed as mean ± SD of three independent experiments (a*, p<0.05 vs. wild mice; b*, p<0.05 vs. HBX transgenic mice).

Article Snippet: Tissue sections were reacted with antibodies against phospho-ATM (Ser1981) (Abgent, San Diego, CA, USA; 1:100) or phospho-Chk2 (Thr68) (Abnova, Taipei, Taiwan) overnight at 4°C using a Vector Elite ABC kit (Vector Laboratories, Burlingame, CA, USA).

Techniques: Western Blot, Control, Plasmid Preparation, Transfection, Immunohistochemical staining, Staining, Labeling, Transgenic Assay