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Image Search Results
Journal: bioRxiv
Article Title: Extracellular vesicles adhere to cells predominantly through the interaction of CD151-associated integrin heterodimers and GM1 with laminin
doi: 10.1101/2024.04.11.589011
Figure Lengend Snippet: GM1 is responsible for the binding of sEVs to laminin. (A) (top) Schematic diagram of gangliosides and (bottom) chemical structure of GM1. (B) Dot blotting of GM1 and GM3 in PC3 cells and PC3-derived sEVs. (C) Western blot analysis of integrin subunits in B78 cell lines with abundant expression of one type of ganglioside—GM1, GM2, GM3, GD2 or GD2/GD3—and sEVs derived from these cells. (D and E) The numbers of sEVs attached to glass coated with laminin (D) and the numbers normalized to the ratio of integrin α6/CD81 in the sEVs (E). (F and G) The numbers of sEVs attached to glass coated with fibronectin (F) and the numbers normalized to the ratio of integrin α5/CD81 in the sEVs (G). (H) Single-particle fluorescence images of DMPC-liposomes containing GM1, GM2, GM3, GD1a, GD2, or GD3 on glass coated with fibronectin or laminin. (I and J) The numbers of liposomes attached to glass coated with laminin (I) or fibronectin (J). The data are presented as the mean ± SE. * indicates a significant difference (p<0.001) according to Welch’s t-test (two-sided).
Article Snippet: The gangliosides GM1 (bovine brain),
Techniques: Binding Assay, Derivative Assay, Western Blot, Expressing, Single Particle, Fluorescence, Liposomes