Journal: International journal of biological macromolecules

Article Title: A novel platform for drug testing: Biomimetic three-dimensional hyaluronic acid-based scaffold seeded with human hepatocarcinoma cells.

doi: 10.1016/j.ijbiomac.2021.06.174

Figure Lengend Snippet: Fig. 2. Assessment of the distribution of F-actin in HepG2 cells cultured on 2D support and in HA3P50 scaffold (3D). HepG2 cells were grown on 2D supports and in 3D systems for 1, 7, 14 and 21 days (A) Representative phalloidin-FITC/DAPI merged images for 2D cell culture. (B) Representative phalloidin-FITC/DAPI merged images (up) and phase-contrast images (down) for 3D cell system (green, actin and blue, cell nuclei). The yellow arrows point to the closely grouped cells attached to the scaffold's fibers or plastic support, the orange arrows show cell clusters, the red arrows indicate the cellular aggregates, and white squares indicate the extension of cluster cells. Scale bar: 100 μm. (C) 3D reconstruction images of Z-stacking sections for phalloidin-FITC and DAPI staining of cellularized 3D systems. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Cisplatin and phalloidin-Fluorescein isothiocyanate (FITC), were from Tocris Bioscience (Bio-Techne Ltd., Minneapolis, USA).

Techniques: Cell Culture, Staining