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Image Search Results
Journal: Journal of Physics
Article Title: The distribution of DNA translocation times in solid-state nanopores
doi: 10.1088/0953-8984/22/45/454129
Figure Lengend Snippet: (A) Illustration of linear DNA translocation experiment. (B) Typical 3 kbp DNA translocation events in a 8±2 nm diameter pore in 1.5 MKCl with 30% glycerol at pH 7.5. (C) All events distribution plot of current drop ΔIb vs translocation times td. (D) Selected linear translocation events plot from the data shown in (C).
Article Snippet: The DNA molecules used for the data in this work are from 3 kbp dsDNA (plasmid pSP65) and
Techniques: Translocation Assay
Journal: Journal of Physics
Article Title: The distribution of DNA translocation times in solid-state nanopores
doi: 10.1088/0953-8984/22/45/454129
Figure Lengend Snippet: (A) Event distribution plot of translocation of a DNA ladder (λ cut) that contains a mixture of ~2.17, 4.36, 6.56, 9.42, and 23 kbp DNA. The experiment was performed in 1.0 M KCl with no glycerol. More than 20,000 events are in this event distribution plot. (B) The experiment was performed in 1.6 M KCl with 20% glycerol. Both sets of data were recorded with low pass filter set at 100 kHz. The fitted drifting speed (C) and the diffusion constants (D) as a function of the DNA chain length. All data are measured with 10±2 nm silicon nitride pores and the applied voltage was 120 mV. The error bars for drifting speed in panel C are smaller than the markers for all the data points.
Article Snippet: The DNA molecules used for the data in this work are from 3 kbp dsDNA (plasmid pSP65) and
Techniques: Translocation Assay, Diffusion-based Assay
Journal: Journal of Physics
Article Title: The distribution of DNA translocation times in solid-state nanopores
doi: 10.1088/0953-8984/22/45/454129
Figure Lengend Snippet: (A) Event distribution plot of 3 kbp (LC=1020 nm) DNA translocation as a function of solution viscosity in 1.5 M KCl, pH 7.5, and ψ=120 mV. Insert, left axis: the uncertainty in determine DNA chain length due to random walk ΔL/LDNA( ); right axis: the relative blockade current ΔI/I0 (◆). The error bars are smaller than the symbols. (B) The drifting speed, (C) the diffusion constant, and (D) the calculated drag force as a function of viscosity.
Article Snippet: The DNA molecules used for the data in this work are from 3 kbp dsDNA (plasmid pSP65) and
Techniques: Translocation Assay, Diffusion-based Assay
Journal: Journal of Physics
Article Title: The distribution of DNA translocation times in solid-state nanopores
doi: 10.1088/0953-8984/22/45/454129
Figure Lengend Snippet: (A) Event distribution plot of 3 kbp DNA translocation as a function of applied voltage. Insert, left axis: the uncertainty in determine DNA chain length due to random walk ΔL/LDNA( ); right axis: the relative blockade current ΔI/I0 (◆). The error bars are smaller than the symbols. (B) The drifting speed, (C) the diffusion constant, and (D) the calculated drag force as a function of voltage. The experiment was performed in 1.6 M KCl with 20% glycerol at pH 7.5 in a 8±2 nm silicon nitride pore.
Article Snippet: The DNA molecules used for the data in this work are from 3 kbp dsDNA (plasmid pSP65) and
Techniques: Translocation Assay, Diffusion-based Assay