Journal: Cell Death & Disease

Article Title: Enhanced hexosamine metabolism drives metabolic and signaling networks involving hyaluronan production and O -GlcNAcylation to exacerbate breast cancer

doi: 10.1038/s41419-019-2034-y

Figure Lengend Snippet: mRNA expression of HBP enzymes in primary breast cancer cells

Article Snippet: The TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA) used were as follows: Assay ID: Mm01183874_m1 (GFAT1), Mm00496565_m1 (GFAT2), Mm00834602_mH (GNPNAT1), Mm01144498_m1 (PGM3), Mm01281909_m1 (UAP1), Mm00507317_m1 (OGT), and Mm00452409_m1 (OGA).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Inhibition of the Hexosamine Biosynthetic Pathway by targeting PGM3 causes breast cancer growth arrest and apoptosis

doi: 10.1038/s41419-018-0405-4

Figure Lengend Snippet: a Schematic representation of HBP. b Schematic drawing of the conversion of FR054 (prodrug) into FR051 (active compound). c Poses of the FR051 (upper), Glc N Ac-6-P (middle), and Glc N Ac-1-P (bottom) molecules in the catalytic cleft of PGM3 and their docking scores (kj/mol). d CETSA curves for PGM3 with FR054 measured in MDA-MB-231 cell extracts from 49 to 70 °C. e ITDRF CETSA curves for FR054 and Glc N Ac-6-P in cell extracts at 58 °C. f HPLC quantification of UDP-Glc N Ac in cell extracts of MDA-MB-231 upon FR054 treatment. All data represent the average ± s.d.; * p < 0.05 (Student’s t -test; comparison with FR054-not-treated sample); N = 3

Article Snippet: Equal amounts of proteins were loaded onto 10% SDS–PAGE gels, transferred to nitrocellulose membranes, and analyzed using the following antibodies: PGM3 (#A304-555A, Bethyl Laboratories, Montgomery, TX, USA; 1:5000), vinculin (#sc-5573, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; 1:10000), UAP1 (HPA014659, Sigma-Aldrich; 1:250).

Techniques: Comparison

Journal: Cell Death & Disease

Article Title: Inhibition of the Hexosamine Biosynthetic Pathway by targeting PGM3 causes breast cancer growth arrest and apoptosis

doi: 10.1038/s41419-018-0405-4

Figure Lengend Snippet: Cell death of MDA-MB-231 cells upon treatment with NAGKi and FR054: scheme of treatment ( a ), cell death determined by viable count ( b ), representative images of cells upon treatment ( c ) (4× magnification, 50 μm scale). Efficacy of shRNA silencing of PGM3 enzyme in MDA-MB-231 stable clones, as detected by RNA ( d ) and protein ( e ) levels quantification. f Cell viability of shCTR and shPGM3 clones upon 48 h treatment with FR054 0.5 mM and 1 mM. g , h Cell viability of MDA-MB-231 shCTR and shPGM3 representative clones detected by MTT test ( g ; 48 h FR054 at different doses) and Ann V-FITC staining ( h ; 48 h FR054 0.5 mM). All data represent the average ± s.d.; * p < 0.05, ** p < 0.01 (Student’s t -test; comparison with FR054-not-treated sample); N = 3

Article Snippet: Equal amounts of proteins were loaded onto 10% SDS–PAGE gels, transferred to nitrocellulose membranes, and analyzed using the following antibodies: PGM3 (#A304-555A, Bethyl Laboratories, Montgomery, TX, USA; 1:5000), vinculin (#sc-5573, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; 1:10000), UAP1 (HPA014659, Sigma-Aldrich; 1:250).

Techniques: shRNA, Clone Assay, Staining, Comparison

Journal: BioMed Research International

Article Title: Dynamic Expression of Genes Involved in Proteoglycan/Glycosaminoglycan Metabolism during Skin Development

doi: 10.1155/2018/9873471

Figure Lengend Snippet: Differentially expressed GAG related genes during skin development in mice in comparison to mature skin (p <0.10) based on real-time qPCR.

Article Snippet: , Mm00459270_m1 , , , , , , , , , .

Techniques: Comparison, Modification

Journal: BioMed Research International

Article Title: Dynamic Expression of Genes Involved in Proteoglycan/Glycosaminoglycan Metabolism during Skin Development

doi: 10.1155/2018/9873471

Figure Lengend Snippet: Differentially expressed GAG related genes during skin development in mice in comparison to mature skin (p <0.10) based on gene Chip Mouse Exon 1.0 ST Arrays.

Article Snippet: , Mm00459270_m1 , , , , , , , , , .

Techniques: Comparison, Modification