Journal: PLoS ONE

Article Title: C3HC4-Type RING Finger Protein Nb ZFP1 Is Involved in Growth and Fruit Development in Nicotiana benthamiana

doi: 10.1371/journal.pone.0099352

Figure Lengend Snippet: We selected twenty-five typical C3HC4-type RING finger genes with high similarity from different species, and we analyzed the similarity of their RING finger domains and full-length sequences with NbZFP1 . Phylogenetic trees were generated by the maximum likelihood (ML) method using MEGA4. Bootstrap values from 1000 replicates are indicated at each branch. (a) A maximum likelihood (ML) tree of NbZFP1 and genes from other species was constructed based on RING finger domain sequences. (b) A maximum likelihood (ML) tree of NbZFP1 and genes from other species was constructed based on full-length nucleotide sequences.

Article Snippet: The extracted plasmid, designated pGEX-6p-2- NbZFP1 , was subjected to DNA sequencing by Beijing Genomics Institution, Beijing, China.

Techniques: Generated, Construct

Journal: PLoS ONE

Article Title: C3HC4-Type RING Finger Protein Nb ZFP1 Is Involved in Growth and Fruit Development in Nicotiana benthamiana

doi: 10.1371/journal.pone.0099352

Figure Lengend Snippet: Laser-scanning confocal micrographs showing the fluorescence of leaf cells following infiltration with Agrobacteria carrying pGDG, pGDG- Rac1 , or pGDG- NbZFP1 plasmids expressing GFP, Rac1-GFP, and Nb ZFP1-GFP proteins, respectively. Scale bar = 10 µm. (a) and (b) GFP expressed from pGDG; (c) and (d) GFP expressed from pGDG-Rac1; (e), (f), (g), and (h) Fluorescence expressed from pGDG- NbZFP1 . (a), (c), (e), and (g) show the green channel; (b), (d), and (f) show an overlay of the bright-field and green channels; (h) shows an overlay of the bright-field, green, and red channels.

Article Snippet: The extracted plasmid, designated pGEX-6p-2- NbZFP1 , was subjected to DNA sequencing by Beijing Genomics Institution, Beijing, China.

Techniques: Fluorescence, Expressing

Journal: PLoS ONE

Article Title: C3HC4-Type RING Finger Protein Nb ZFP1 Is Involved in Growth and Fruit Development in Nicotiana benthamiana

doi: 10.1371/journal.pone.0099352

Figure Lengend Snippet: (a) Effect of VIGS on NbZFP1 (in this graph, NbA ) transcription in N. benthamiana . TRV: The expression level of NbZFP1 in plants infected with TRV alone; TRV- A1 : The expression level of NbZFP1 in plants infected with TRV- A1 ( A1 is the C3HC4-type RING finger domain gene NbZFP1 ); TRV- A2 : The expression level of NbZFP1 in plants infected with TRV- A2 (A2 is NbZFP1 lacking the C3HC4-type RING finger domain). (b) The phenotype of VIGS-silenced NbZFP1 and wild type strains. b1, NbZFP1- silenced plant at 20 days after the four-leaf stage; b2, wild type plant at 20 days after the four-leaf stage; b3, NbZFP1- silenced plant at 40 days after the four-leaf stage; b4, wild type plant at 40 days after the four-leaf stage. (c) The fruit phenotype of NbZFP1- silenced plants compared with controls. c1, c2, and c3: The fruit phenotype of non-silenced control plants. c4, c5, and c6: The fruit phenotype of NbZFP1- silenced plants.

Article Snippet: The extracted plasmid, designated pGEX-6p-2- NbZFP1 , was subjected to DNA sequencing by Beijing Genomics Institution, Beijing, China.

Techniques: Expressing, Infection, Control

Journal: PLoS ONE

Article Title: C3HC4-Type RING Finger Protein Nb ZFP1 Is Involved in Growth and Fruit Development in Nicotiana benthamiana

doi: 10.1371/journal.pone.0099352

Figure Lengend Snippet: (a) The fruit phenotype of transgenic lines compared with wild type plants. a1 and a2, the fruit phenotype of transgenic plants. a3 and a4, the fruit phenotype of wild type plants. (b) The phenotype of T 1 single copy lines and wild type lines. b1, T 1 generation single copy transgenic strains at 20 days after the four-leaf stage; b2, wild type strains at 20 days after the four-leaf stage; b3: T 1 single copy transgenic strains at 50 days after the four-leaf stage; b4, wild type strains at 50 days after the four-leaf stage. (c) The height of single copy NbZFP1 transgenic plants compared with the height of wild type plants at different times. (d) The internode lengths of single copy NbZFP1 transgenic plants compared with the wild type internode length, 30 plants were measured. Asterisks indicate significant differences from the wild type: *** P<0.001.

Article Snippet: The extracted plasmid, designated pGEX-6p-2- NbZFP1 , was subjected to DNA sequencing by Beijing Genomics Institution, Beijing, China.

Techniques: Transgenic Assay