pgex 6p-1 vector Search Results


arginines 8r  (Cytiva Europe)
95
Cytiva Europe arginines 8r
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Arginines 8r, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/PGEX-6P-1+Vector/pmc05161512-165-9-17
Average 95 stars, based on 1 article reviews
arginines 8r - by Bioz Stars, 2026-09
95/100 stars
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iptg-inducible pgex-6p1 expression vector for gst and human cb1 receptor fusion protein  (CancerTools Org)
N/A
Purified plasmid DNA containing the IPTG inducible coding sequence for the polypeptide spanning amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor fused to the C-terminus of the glutathione S-transferase
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vector pgex 6p1  (Cytiva Europe)
96
Cytiva Europe vector pgex 6p1
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Vector Pgex 6p1, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/PGEX-6P-1+Vector/10__1074_slash_jbc__c600051200-19-22-24
Average 96 stars, based on 1 article reviews
vector pgex 6p1 - by Bioz Stars, 2026-09
96/100 stars
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the bacterial expression vector pgex-6p-1  (BIO-CAT Inc)
90
BIO-CAT Inc the bacterial expression vector pgex-6p-1
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
The Bacterial Expression Vector Pgex 6p 1, supplied by BIO-CAT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/the+bacterial+expression+vector+pgex+6p+1/pmc08014119__ANIE___9999___0___s001-112-16-23
Average 90 stars, based on 1 article reviews
the bacterial expression vector pgex-6p-1 - by Bioz Stars, 2026-09
90/100 stars
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pgex-6p1 multicloning site expression vector of gst protein fusion under the control of the laci-tac promoter, ampr  (Promega)
90
Promega pgex-6p1 multicloning site expression vector of gst protein fusion under the control of the laci-tac promoter, ampr
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Pgex 6p1 Multicloning Site Expression Vector Of Gst Protein Fusion Under The Control Of The Laci Tac Promoter, Ampr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/pgex+6p1+multicloning+site+expression+vector+of+gst+protein+fusion+under+the+control+of+the+laci+tac+promoter++ampr/pm16963230-67-243-244
Average 90 stars, based on 1 article reviews
pgex-6p1 multicloning site expression vector of gst protein fusion under the control of the laci-tac promoter, ampr - by Bioz Stars, 2026-09
90/100 stars
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hr23b-ubl wt (pgex6p1) with n-terminal gst tag and a prescission protease cleavage site  (BioCat GmbH)
90
BioCat GmbH hr23b-ubl wt (pgex6p1) with n-terminal gst tag and a prescission protease cleavage site
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Hr23b Ubl Wt (Pgex6p1) With N Terminal Gst Tag And A Prescission Protease Cleavage Site, supplied by BioCat GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/pgex+6p1+vector+harboring+a+human+rhinovirus+3c+excision+site+and+a+glutathione+transferase++gst++tag+at+the+n+terminal+region/pmc10241913-326-0-16
Average 90 stars, based on 1 article reviews
hr23b-ubl wt (pgex6p1) with n-terminal gst tag and a prescission protease cleavage site - by Bioz Stars, 2026-09
90/100 stars
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pgex-6p-1 vector  (Clonexpress inc)
90
Clonexpress inc pgex-6p-1 vector
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Pgex 6p 1 Vector, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/pgex+6p+1+vector/pm36840945-243-4-7
Average 90 stars, based on 1 article reviews
pgex-6p-1 vector - by Bioz Stars, 2026-09
90/100 stars
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expression vector pgex-6p-1  (Beijing CWBio)
90
Beijing CWBio expression vector pgex-6p-1
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Expression Vector Pgex 6p 1, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/expression+vector+pgex+6p+1/pm34599934-40-11-25
Average 90 stars, based on 1 article reviews
expression vector pgex-6p-1 - by Bioz Stars, 2026-09
90/100 stars
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pgex-6p-1 vector  (Cytiva Europe)
95
Cytiva Europe pgex-6p-1 vector
Loss <t>of</t> <t>Cited2</t> Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant <t>8R-CITED2</t> protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.
Pgex 6p 1 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p-1+vector/PGEX-6P-1+Vector/custom%4028954648%4042372723
Average 95 stars, based on 1 article reviews
pgex-6p-1 vector - by Bioz Stars, 2026-09
95/100 stars
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Image Search Results


Loss of Cited2 Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant 8R-CITED2 protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.

Journal: Stem Cell Reports

Article Title: CITED2 Cooperates with ISL1 and Promotes Cardiac Differentiation of Mouse Embryonic Stem Cells

doi: 10.1016/j.stemcr.2016.10.002

Figure Lengend Snippet: Loss of Cited2 Impairs Expression of Genes Specifying Cardiac Mesoderm (A) Percentage of colonies with beating foci derived from C2 fl/fl and C2 Δ/Δ [LA11] ESC at D8 and D10 of differentiation. (B) Percentage of colonies with beating foci counted at 8 and 10 days after the initiation of differentiation in cell cultures derived from C2 fl/fl [Cre] ESC treated with ethanol or 4HT at D0 of differentiation, and with 4HT at D0 of differentiation and supplemented with recombinant 8R-CITED2 protein at D2 of differentiation (4HT + 8R-CITED2). (C) Expression of Activin A and Nodal determined by qPCR at D2 and D3 of differentiation in cultures derived from C2 fl/fl [Cre] ESC treated with 4HT or ethanol at D0 for 48 hr. (D) Expression of mesoderm markers ( Brachyury and Mesp1 ) at D2, D3, D5, and D12 of differentiation in cells generated from C2 fl/fl [Cre] ESC treated as described in ( B). (E) Expression of Isl1 , Gata4 , Nkx2.5 , and Tbx5 in cell cultures as described in (D). The inserts for Isl1 and Tbx5 detail the expression of these genes at D5 and D3, respectively. (F) Relative expression of Brachyury , Mesp1 , Isl1 , Gata4 , Nkx2.5 , and Tbx5 determined by qPCR at D5 of differentiation in cultures derived from untreated C2 fl/fl and C2 Δ/Δ [LA11] ESC, and C2 Δ/Δ [LA11] ESC supplemented with the recombinant 8R-CITED2 protein (C2 Δ/Δ [LA11] + 8R-CITED2) at D2 of differentiation for 48 hr. Gene expression in C2 fl/fl ESC was set to 1. (G) Enrichment of Isl1 genomic regions in extracts of E14/T ESC-derived cells at D5 by ChIP assays with anti-CITED2 or control (immunoglobulin G) polyclonal antibodies. Results are presented as the mean ± SEM of three independent biological experiments.

Article Snippet: Full-length human CITED2 cDNA and an oligonucleotide encoding eight arginines (8R) were cloned into the pGEX6P1 vector (GE Healthcare Life Sciences) to express a chimeric protein consisting of the GST in fusion with the 8R domain and CITED2 (termed GST-8R-CITED2, ).

Techniques: Expressing, Derivative Assay, Recombinant, Generated, Gene Expression, Control