Journal: Microbiology

Article Title: ChpC controls twitching motility-mediated expansion of Pseudomonas aeruginosa biofilms in response to serum albumin, mucin and oligopeptides

doi: 10.1099/mic.0.000911

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: [ 60 ] pGEMTeasy AmpR; E. coli cloning vector Promega pGemCLF AmpR; A 1149 bp PCR amplicon from the 5′ end of chpC (CLF) and inserted into pGEMTeasy This study pGemCRF AmpR; A 1238 bp fragment PCR amplicon from the 3′ end of chpC (CRF) and inserted into pGEMTeasy This study pCRFV KanR; Bam HI- Kpn I fragment from pGEMCRF inserted into pOK12 This study pOKCDEL Kan, TcR; The concatamerized fragment including the Bgl II- Kpn I fragment from pGEMCLF and the 1638 bp KpnI fragment from pLM1 with a frt flanked TcR cassette, inserted into the Kpn I and Bam HI sites of pCRFV fragment; This study pRIC380 AmpR; P. aeruginosa suicide vector [ 58 ] pCDEL AmpR, TcR; A 4008 bp Spe I fragment from pOKCDEL containing the chpC deletion construct inserted into the Spe I site in pRIC380 against sacB This study Open in a separate window Strains and plasmids used in this study The PAK genome (NCBI number accession number {"type":"entrez-nucleotide","attrs":{"text":"LR657304","term_id":"1708254220","term_text":"LR657304"}} LR657304 [ 48 ];) was used to search for methyl-accepting chemotaxis protein (MCP) and solute-binding protein (SBP) orthologs.

Techniques: Plasmid Preparation, Amplification, Clone Assay, Construct