pgem-3 rna expression vector Search Results


pgem3-trna sec  (Promega)
90
Promega pgem3-trna sec
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Pgem3 Trna Sec, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pmc00060083-60-20-15?v=Promega
Average 90 stars, based on 1 article reviews
pgem3-trna sec - by Bioz Stars, 2026-07
90/100 stars
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plasmid pgem3-hcv2b-ires  (Promega)
90
Promega plasmid pgem3-hcv2b-ires
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Plasmid Pgem3 Hcv2b Ires, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/us09068969-563-1-32?v=Promega
Average 90 stars, based on 1 article reviews
plasmid pgem3-hcv2b-ires - by Bioz Stars, 2026-07
90/100 stars
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t7 rna polymerase  (Promega)
90
Promega t7 rna polymerase
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
T7 Rna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm03398852-117-6-12?v=Promega
Average 90 stars, based on 1 article reviews
t7 rna polymerase - by Bioz Stars, 2026-07
90/100 stars
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pcmv5  (Promega)
90
Promega pcmv5
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Pcmv5, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm11971870-151-25-28?v=Promega
Average 90 stars, based on 1 article reviews
pcmv5 - by Bioz Stars, 2026-07
90/100 stars
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psp64/poly(a) vector  (Promega)
90
Promega psp64/poly(a) vector
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Psp64/Poly(a) Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/10__1074_slash_jbc__m206981200-110-13-15?v=Promega
Average 90 stars, based on 1 article reviews
psp64/poly(a) vector - by Bioz Stars, 2026-07
90/100 stars
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rna probes  (Promega)
90
Promega rna probes
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Rna Probes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm08377377-30-5-39?v=Promega
Average 90 stars, based on 1 article reviews
rna probes - by Bioz Stars, 2026-07
90/100 stars
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pgem -3zf vector  (Promega)
90
Promega pgem -3zf vector
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Pgem 3zf Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/10__1203_slash_pdr__0b013e318163a215-64-42-45?v=Promega
Average 90 stars, based on 1 article reviews
pgem -3zf vector - by Bioz Stars, 2026-07
90/100 stars
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psp65  (Promega)
90
Promega psp65
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Psp65, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm02484713-142-2-23?v=Promega
Average 90 stars, based on 1 article reviews
psp65 - by Bioz Stars, 2026-07
90/100 stars
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crna probes  (Promega)
90
Promega crna probes
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Crna Probes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm21929693-131-2-49?v=Promega
Average 90 stars, based on 1 article reviews
crna probes - by Bioz Stars, 2026-07
90/100 stars
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rabbit reticulocyte coupled-transcription/translation system  (Promega)
90
Promega rabbit reticulocyte coupled-transcription/translation system
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Rabbit Reticulocyte Coupled Transcription/Translation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pm09388245-168-26-28?v=Promega
Average 90 stars, based on 1 article reviews
rabbit reticulocyte coupled-transcription/translation system - by Bioz Stars, 2026-07
90/100 stars
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pg3o  (Promega)
90
Promega pg3o
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Pg3o, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/pmc00104825-176-17-32?v=Promega
Average 90 stars, based on 1 article reviews
pg3o - by Bioz Stars, 2026-07
90/100 stars
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amersham synthesis kit  (Amersham Life Sciences Inc)
86
Amersham Life Sciences Inc amersham synthesis kit
NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and <t>pGEM3-tRNASec</t> or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.
Amersham Synthesis Kit, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-3+rna+expression+vector/10__1074_slash_jbc__272__5__3042-61-29-29?v=Amersham+Life+Sciences+Inc
Average 86 stars, based on 1 article reviews
amersham synthesis kit - by Bioz Stars, 2026-07
86/100 stars
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Image Search Results


NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and pGEM3-tRNASec or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.

Journal:

Article Title: Overexpression of cellular glutathione peroxidase rescues homocyst(e)ine-induced endothelial dysfunction

doi: 10.1073/pnas.231428998

Figure Lengend Snippet: NO release from BAEC into the medium over 2 h. Cells were transfected with pCMV-GPx-1, pCMV-SelD, and pGEM3-tRNASec or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. The buffer was then changed to a thiol-free buffer, and DAF-2 fluorescence in the supernatant was measured after stimulation with BK 10−5 mol/liter for 2 h as described in Materials and Methods. n = four experiments. *, P < 0.05 vs. control; **, P < 0.005 vs. control.

Article Snippet: An 800-bp Eco RI– Hin dIII fragment had been cloned into the expression vector pGEM3 (Promega); this vector is denoted pGEM3-tRNA Sec .

Techniques: Transfection, Fluorescence

cGMP accumulation in BASMC after short-term coculture with BAEC. BAEC were grown in transwell inserts, transfected with pCMV-GPx-1, pCMV-SelD, and pGEM3-tRNASec or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. BAEC were then transferred for coincubation with BASMC, incubated with 3-isobutyl-1-methylxanthine for 15 min, and then stimulated with BK 10−5 mol/liter for 60 s. cGMP was measured in the ethanol extract of BASMC by using an enzyme immunoassay. n = three experiments. *, P < 0.01 vs. BK-stimulated control; **, P < 0.001 vs. BK-stimulated control.

Journal:

Article Title: Overexpression of cellular glutathione peroxidase rescues homocyst(e)ine-induced endothelial dysfunction

doi: 10.1073/pnas.231428998

Figure Lengend Snippet: cGMP accumulation in BASMC after short-term coculture with BAEC. BAEC were grown in transwell inserts, transfected with pCMV-GPx-1, pCMV-SelD, and pGEM3-tRNASec or sham-transfected and preincubated with the indicated concentrations of dl-homocysteine or l-cysteine for 4 h. BAEC were then transferred for coincubation with BASMC, incubated with 3-isobutyl-1-methylxanthine for 15 min, and then stimulated with BK 10−5 mol/liter for 60 s. cGMP was measured in the ethanol extract of BASMC by using an enzyme immunoassay. n = three experiments. *, P < 0.01 vs. BK-stimulated control; **, P < 0.001 vs. BK-stimulated control.

Article Snippet: An 800-bp Eco RI– Hin dIII fragment had been cloned into the expression vector pGEM3 (Promega); this vector is denoted pGEM3-tRNA Sec .

Techniques: Transfection, Incubation, Enzyme-linked Immunosorbent Assay