Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

Journal: Pediatric research

Article Title: Lipocalin-2 and calprotectin as stool biomarkers for predicting necrotizing enterocolitis in premature neonates.

doi: 10.1038/s41390-021-01680-7

Figure Lengend Snippet: Fig. 1 Detection of CALPRO, LCN2, and HAPTO in the stools of NEC and non-NEC premature infants by ELISA. Stool samples analyzed were those collected over a 10-day period preceding NEC diagnosis (round symbols) and in matched non-NEC infants (square symbols). Simple linear regression for the levels of biomarkers in the stools were calculated for both NEC and non-NEC with R2 values varying between 0.02 and 0.0003 while the slopes were considered nonsignificant for all. For simplicity, only the regression line (black line, R2) for NEC samples are shown (left panels). The biomarker data were then subjected to ROC curve analyses (right panels) showing sensitivity and specificity values and cut-off evaluation (dotted lines *, left panels).

Article Snippet: Calprotectin (CALPRO) ELISA (ALPCO cat # 30-CALHU-E01), prostaglandin E2 (PGE2) ELISA (ALPCO cat # 74-PG2HU-E05), hemaglobin/haptoglobin complex (HAPTO-HEMO) ELISA (ALPCO cat# 30-HAPHU-E01), lysozyme (LYZ) ELISA (ALPCO Cat # 30-LYSHU-E01), osteoprotegerin (OPGN) ELISA (Biomedica Medizinprodukte, Cat# BI-20403, Vienna, Austria), and lipocalin2 (LCN2/NGAL) ELISA (Epitope Diagnostics Inc., Cat# KRT-853, San Diego, CA) were all provided at no cost by ALPCO Immunoassay.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: International Journal of Nanomedicine

Article Title: A glimpse into the interactions of cells in a microenvironment: the modulation of T cells by mesenchymal stem cells

doi: 10.2147/IJN.S50767

Figure Lengend Snippet: Cell–cell analysis of mesenchymal stem cell (MSC)–T cell microenvironment interaction. ( A ) Schematic of microwell array assay for probing immunosuppressive effects of MSCs on cocultured CD4 T cells. ( B ) Representative image of CD4 T cells (round cells) and MSCs (yellow arrow) in microwells. ( C ) Immunosuppressive effect of MSCs on T cell proliferation. Percentage increase in each selected well is defined as the difference between the number of T cells at 48 hours and the initial number of T cells, divided by the initial number of T cells. ( D ) Representative plot of secretory responses measured by microengraving from microwells containing CD4 T cells cocultured with (+) or without (−) MSCs for 48 hours. Rates of secretion: * P < 0.05, ** P < 0.01; one-tailed Mann–Whitney U test. Data are representative of three independent experiments. Abbreviations: PGE2,prostaglandin E2; IL-10, interleukin 10; TGF-1, transforming growth factor 1.

Article Snippet: Monoclonal antibodies certified for ELISpot were used to capture and detect IL-10, PGE2, and TGF-1 (R&D Systems, Minneapolis, MN, USA).

Techniques: Cell Analysis, One-tailed Test, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Impact of Mechanical Load on the Expression Profile of Synovial Fibroblasts from Patients with and without Osteoarthritis

doi: 10.3390/ijms20030585

Figure Lengend Snippet: Impact of static compressive force application on the expression of proinflammatory genes. Gene expression and protein secretion of ( a ) TNF-α, ( b ) COX-2/PG-E2 and ( c ) IL-6 of N-SF and OA-SF after 48 h with or without static compressive force application. AU: arbitrary units; RT-qPCR: n = 9; ELISA: n = 6. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤0.001. Statistics: Welch-corrected ANOVA with Games-Howell post-hoc-tests.

Article Snippet: For quantification of tumour necrosis factor α (TNFα) and prostaglandin E2 (PGE2), interleukin-6 (IL-6), collagen-1 (COL-1) and fibronectin (FN-1) protein secretion into the synovial fibroblast supernatant, we used commercially available ELISA kits according to the manufacturers’ instructions (TNFα: EK0525, Boster Biological Technology; PGE2: 514010; Cayman chemical; IL-6: EK0410 Boster Biological Technology; COL-1: ab210966, Abcam; FN-1: EK0349, Boster Biological Technology).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay