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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: High-Density Real-Time PCR-Based in Vivo Toxicogenomic Screen to Predict Organ-Specific Toxicity
doi: 10.3390/ijms12096116
Figure Lengend Snippet: List of marker genes, their references and Taqman probes used in this study.
Article Snippet: 22 , Pgam2 , phosphoglycerate mutase 2 , NM_018870.3 ,
Techniques: Marker, Binding Assay, Ubiquitin Proteomics, Inhibition, Control
Journal: Oncotarget
Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.
doi: 10.18632/oncotarget.4044
Figure Lengend Snippet: Figure 6: Correlation of ActD-stimulated changes of PGAM localization in NSCLC cells with blockade of the cell cycle cell in G2 phase. Upper panel: the PGAM2 localization in the cells incubated with 0.04 µg/ml (low) or 2.5 µg/ml (high) actinomycin D (ActD). Lower panel: the cells in G1 (red) and G1/S (yellow) phase in control conditions and blockade of the ActD-treated cells in G2 phase (green fluorescence) as determined by FUCCI Cell Cycle Sensor. Bar=10 µm.
Article Snippet: NHS-SS-Diazirine cross-linker (spacer arm length 1.35 nm) was from Thermo Scientific Pierce; protein G-agarose from Roche; siRNA-A (sc-37007) and
Techniques: Incubation, Control, Fluorescence
Journal: Oncotarget
Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.
doi: 10.18632/oncotarget.4044
Figure Lengend Snippet: Figure 7: The effects of KLN-205 cells transfection with PGAM2. The cells were transfected with PGAM2-FITC in normal conditions and in the presence of RNase. As a control, cells were transfected with BSA-FITC. Bar=10 µm.
Article Snippet: NHS-SS-Diazirine cross-linker (spacer arm length 1.35 nm) was from Thermo Scientific Pierce; protein G-agarose from Roche; siRNA-A (sc-37007) and
Techniques: Transfection, Control
Journal: Oncotarget
Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.
doi: 10.18632/oncotarget.4044
Figure Lengend Snippet: Figure 8: The effects of PGAM2 silencing on nucleolar morphology and new RNA production in KLN-205 cells. A. in PGAM2-silenced cells, unlike control cells, stained with propidium iodide (red), only one centrally located nucleolus can be found and nucleolar PGAM staining (green) is undetectable; B. the level of new RNA (red) synthesis in PGAM2-silenced cells is reduced as compared to control cells. To visualize DNA the cells were counterstained with Hoechst 33342 (blue). Bar=10 µm.
Article Snippet: NHS-SS-Diazirine cross-linker (spacer arm length 1.35 nm) was from Thermo Scientific Pierce; protein G-agarose from Roche; siRNA-A (sc-37007) and
Techniques: Control, Staining