Journal: Nucleic Acids Research

Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations

doi: 10.1093/nar/gkaf532

Figure Lengend Snippet: tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

Article Snippet: The following day, cells were transfected with 50 ng of the indicated sup-tRNA plasmid (or an empty vector), 50 ng of p53-dependent Fluc reporter plasmid PG13-luc (a gift from Bert Vogelstein; Addgene Plasmid # 16442; http://n2t.net/addgene:16442; RRID:ADDGENE_16442 ), and 15 ng of pLX313- Renilla .

Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay

Journal: Marine Drugs

Article Title: Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro

doi: 10.3390/md14070133

Figure Lengend Snippet: Alkaloids 1 – 8 and their effect on EGF-induced neoplastic transformation of JB6 P + Cl41 cells. ( A ) Structures of guanidine alkaloids 1 – 8 isolated from the marine sponge Monanchora pulchra ; ( B ) representative pictures of microscopic fields of EGF-induced colonies of JB6 P + Cl41 cells in soft agar treated with urupocidin A ( 6 ) at the indicated concentrations; ( C ) Inhibition of EGF-induced neoplastic transformation of JB6 P + Cl41 cells by compounds 1 – 4 , 7 , and 8 . INCC 50 —concentration leading to a 50% inhibition of colonies formation. Ratios of IC 50 /INCC 50 were calculated using the IC 50 values from generated by using the MTT assay. * indicates a statistically significant difference ( p < 0.05) from the control value.

Article Snippet: The JB6 P + Cl41 mouse epidermal cell line and its stable transfectants, JB6-Luc AP-1 and JB6-Luc p53 (PG-13), cells were kindly provided by Prof. Zigang Dong, Hormel Institute, University of Minnesota, MN, USA.

Techniques: Transformation Assay, Isolation, Inhibition, Concentration Assay, Generated, MTT Assay, Control

Journal: Marine Drugs

Article Title: Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro

doi: 10.3390/md14070133

Figure Lengend Snippet: IC 50 of alkaloids 1 – 8 in human cancer HeLa cells and mouse non-malignant JB6 P + Cl41 cell lines after 48 h of treatment.

Article Snippet: The JB6 P + Cl41 mouse epidermal cell line and its stable transfectants, JB6-Luc AP-1 and JB6-Luc p53 (PG-13), cells were kindly provided by Prof. Zigang Dong, Hormel Institute, University of Minnesota, MN, USA.

Techniques: Activity Assay

Journal: Marine Drugs

Article Title: Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro

doi: 10.3390/md14070133

Figure Lengend Snippet: Effect of alkaloids 1 – 8 on MAPK/AP-1 signaling. ( A ) Effect on basal AP-1-dependent transcriptional activity ( ○ ) and viability (□) of JB6 Cl41 cells stably expressing a luciferase reporter gene controlled by the AP-1 DNA binding sequence after 12 h of treatment. Cell viability was measured using MTT assay; ( B ) effect on the activation of JNK1/2 and ERK1/2. JB6 P + Cl41 cells were treated with the compounds 1 – 8 at the indicated concentrations for 48 h, and the level of protein expression was assessed by Western blotting. The intensities of p-JNK1/2 and p-ERK1/2 signals were quantified with Quantity One 4.6 software (Bio-Rad, Hercules, CA, USA) and normalized against the signals of total JNK1/2 and ERK1/2, correspondently; and ( C ) the effect of SP600125 (specific JNK1/2 inhibitor) on the survival of JB6 P + Cl41 cells treated with compounds 1 – 8 . Cells were co-treated with different concentrations of the individual drugs or their combination for 48 h. Cell viability was measured by MTT assay and the combinational index (CI) values were calculated with CompuSyn software (v.1.0., ComboSyn Inc., Paramus, NJ, USA) using the Chou-Talalay method. The ratio of the substances and the effects are presented in the . * indicates a statistically significant difference ( p < 0.05) from the control value.

Article Snippet: The JB6 P + Cl41 mouse epidermal cell line and its stable transfectants, JB6-Luc AP-1 and JB6-Luc p53 (PG-13), cells were kindly provided by Prof. Zigang Dong, Hormel Institute, University of Minnesota, MN, USA.

Techniques: Activity Assay, Stable Transfection, Expressing, Luciferase, Binding Assay, Sequencing, MTT Assay, Activation Assay, Western Blot, Software, Control

Journal: Marine Drugs

Article Title: Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro

doi: 10.3390/md14070133

Figure Lengend Snippet: Effect alkaloids 1 – 8 on basal p53-dependent transcriptional activity ( ○ ) and viability (□) of JB6 Cl41 cells stably expressing a luciferase reporter gene controlled by the p53 DNA binding sequence after 12 h of treatment. Cell viability was measured using MTT assay. * indicates a statistically significant difference ( p < 0.05) from the control value.

Article Snippet: The JB6 P + Cl41 mouse epidermal cell line and its stable transfectants, JB6-Luc AP-1 and JB6-Luc p53 (PG-13), cells were kindly provided by Prof. Zigang Dong, Hormel Institute, University of Minnesota, MN, USA.

Techniques: Activity Assay, Stable Transfection, Expressing, Luciferase, Binding Assay, Sequencing, MTT Assay, Control

Journal: Marine Drugs

Article Title: Guanidine Alkaloids from the Marine Sponge Monanchora pulchra Show Cytotoxic Properties and Prevent EGF-Induced Neoplastic Transformation in Vitro

doi: 10.3390/md14070133

Figure Lengend Snippet: Summary of observed effects of compounds 1 – 8 .

Article Snippet: The JB6 P + Cl41 mouse epidermal cell line and its stable transfectants, JB6-Luc AP-1 and JB6-Luc p53 (PG-13), cells were kindly provided by Prof. Zigang Dong, Hormel Institute, University of Minnesota, MN, USA.

Techniques: Activity Assay, Phospho-proteomics, Activation Assay

Journal: Cell Reports Medicine

Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells

doi: 10.1016/j.xcrm.2024.101526

Figure Lengend Snippet:

Article Snippet: Plat-A, Plat-E, and PG13 cells were cultured in DMEM (Nacalai Tesque) with 10% FBS.

Techniques: Purification, Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Antibody Labeling, Mass Cytometry, Mutagenesis, Software, Imaging