pff Search Results


93
StressMarq α syn pff
α Syn Pff, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq human k18 p301l fibrils
Human K18 P301l Fibrils, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq pffs
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Pffs, supplied by StressMarq, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pffs - by Bioz Stars, 2026-03
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93
StressMarq mouse 2n4r fibrils
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Mouse 2n4r Fibrils, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq human ht40 fibrils
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Human Ht40 Fibrils, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq dgae fibrils
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Dgae Fibrils, supplied by StressMarq, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq recombinant tau 441 2n4r p301s mutant protein
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Recombinant Tau 441 2n4r P301s Mutant Protein, supplied by StressMarq, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq wild type preformed fibrils stressmarq biosciences spr
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Wild Type Preformed Fibrils Stressmarq Biosciences Spr, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq α syn pffs
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
α Syn Pffs, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq p301smutant pff cat no spr 471 both stressmarq biosciences
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
P301smutant Pff Cat No Spr 471 Both Stressmarq Biosciences, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq alpha synuclein a53t mutant preformed fibrils
LRP1 <t>regulates</t> <t>α-Syn</t> uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils <t>(PFFs)</t> used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001
Alpha Synuclein A53t Mutant Preformed Fibrils, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LRP1 regulates α-Syn uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils (PFFs) used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Molecular Neurodegeneration

Article Title: LRP1 is a neuronal receptor for α-synuclein uptake and spread

doi: 10.1186/s13024-022-00560-w

Figure Lengend Snippet: LRP1 regulates α-Syn uptake in iPSNs. a and b , α-Syn uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (100 nM, 3 h of treatment). c Representative images of WT or LRP1 -KO iPSNs after α-Syn uptake. Scale bars, 20 μm. d and e , Transferrin (Tfn) uptake in WT and LRP1 -KO iPSNs measured by flow cytometry (300 nM, 3 h of treatment). f Uptake of α-Syn and Tfn in the presence of increasing concentrations of RAP. g EM images showing the structure of α-Syn oligomers and preformed fibrils (PFFs) used in panel h. Scale bars, 200 nm. h Uptake of α-Syn oligomers and PFFs in WT and LRP1 -KO iPSNs (100 nM monomer equivalent, 3 h of treatment). All experiments in ( a , b , d , e , f and h ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as mean ± s.d. with individual data points shown. Data were analyzed by One-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The morphology of α-Syn species was confirmed by negative stain transmission electron microscopy. α-Syn oligomers (StressMarq, cat# SPR-484) and PFFs (StressMarq, cat# SPR-322-C) were prepared at 25 µM in water.

Techniques: Flow Cytometry