Journal: Stem Cell Research & Therapy

Article Title: Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

doi: 10.1186/s13287-022-02980-3

Figure Lengend Snippet: PF-127/hADSCs-Exos complexes allow the stable release of hADSCs-Exos. a Schematic representation of the experimental approach. b The cumulative release ratio of hADSCs-Exos was detected by protein quantification. c PKH26-labeled hADSCs-Exos retention in the site of the wound. Scale bar = 20 μm. d Local magnification of the focal zone in ( c ). PKH26-labeled hADSCs-Exos (red) and DAPI (blue). Arrows indicate hADSCs-Exos. Scale bar = 5 μm

Article Snippet: In vitro, to detect hADSCs-Exos released from the PF-127 hydrogel, we mixed hADSCs-Exos with 25% and 30% PF-127 at 4 ℃, then we put them in the transwell upper compartment placed in a 24-well plate (3422, Corning, Corning, NY, USA) at 37 °C, and put 100 ul dPBS in the lower cells.

Techniques: Labeling

Journal: Stem Cell Research & Therapy

Article Title: Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

doi: 10.1186/s13287-022-02980-3

Figure Lengend Snippet: PF-127/hADSCs-Exos complexes promote cutaneous wound healing. a Representative images of the skin wound healing in each group on days 0, 1, 4, 7, 10, and 13. b Quantitation data of the ratio of the wound healing in each group. c Wounded skin sections stained with H&E in diverse groups on days 4, 7, 10, and 13 post-wounding. A representative biopsy from F127/hADSCs-Exo groups showed a normal intact hair follicle (blue arrow). Scale bar = 50 μm. d Representative H&E staining images of full-thickness wounds on days 13. Scale bar = 1250 μm. e Length between wound edges was calculated. In b and e , data are shown as mean ± SEM; n = 6 for each group. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, **** p -value < 0.0001 vs vehicle control group

Article Snippet: In vitro, to detect hADSCs-Exos released from the PF-127 hydrogel, we mixed hADSCs-Exos with 25% and 30% PF-127 at 4 ℃, then we put them in the transwell upper compartment placed in a 24-well plate (3422, Corning, Corning, NY, USA) at 37 °C, and put 100 ul dPBS in the lower cells.

Techniques: Quantitation Assay, Staining

Journal: Stem Cell Research & Therapy

Article Title: Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

doi: 10.1186/s13287-022-02980-3

Figure Lengend Snippet: PF-127/hADSCs-Exos improves cell proliferation and angiogenesis. a Images illustrate Ki67 immunohistochemistry staining on days 4 and 7. Scale bar = 20 μm. b Quantification of the number of Ki67 positive cells in the wound area. c α-SMA staining of myofibroblasts in wound bed days 4 and 7 post-operative. Scale bar = 20 µm. d Quantification of α-SMA stained tissues. e Representative images of IHC of CD31 on days 4 and 7 wound sections. Scale bar = 20 µm. f Microvessel density analyses of diverse treatment days 4 and 7 ( n = 6). In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, **** p -value < 0.0001 vs vehicle control group

Article Snippet: In vitro, to detect hADSCs-Exos released from the PF-127 hydrogel, we mixed hADSCs-Exos with 25% and 30% PF-127 at 4 ℃, then we put them in the transwell upper compartment placed in a 24-well plate (3422, Corning, Corning, NY, USA) at 37 °C, and put 100 ul dPBS in the lower cells.

Techniques: Immunohistochemistry, Staining

Journal: Stem Cell Research & Therapy

Article Title: Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

doi: 10.1186/s13287-022-02980-3

Figure Lengend Snippet: Histochemical analysis of collagen deposition in wounds treated by PF-127/hADSCs-Exos. a Masson staining of collagens deposition in different groups on days 4, 7, 10, and 13 post-wounding. Scale bar = 50 µm. b Quantification of collagens deposition in different groups. c Immunohistochemistry staining images for collagen I at 7, 13 days post-wounding. Scale bar = 20 µm. d Relative density analysis of collagen I. e Immunohistochemistry staining images for collagen III at 7, 13 days post-wounding. Scale bar = 20 µm. f Relative density analysis of collagen III. g Relative density analysis of collagen I/collagen III ratios at 7 and 13 days after treatment. In b, d , f, and g , data are shown as mean ± SEM; n = 6 for each group. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, **** p -value < 0.0001 vs vehicle control group

Article Snippet: In vitro, to detect hADSCs-Exos released from the PF-127 hydrogel, we mixed hADSCs-Exos with 25% and 30% PF-127 at 4 ℃, then we put them in the transwell upper compartment placed in a 24-well plate (3422, Corning, Corning, NY, USA) at 37 °C, and put 100 ul dPBS in the lower cells.

Techniques: Staining, Immunohistochemistry

Journal: Stem Cell Research & Therapy

Article Title: Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration

doi: 10.1186/s13287-022-02980-3

Figure Lengend Snippet: PF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group

Article Snippet: In vitro, to detect hADSCs-Exos released from the PF-127 hydrogel, we mixed hADSCs-Exos with 25% and 30% PF-127 at 4 ℃, then we put them in the transwell upper compartment placed in a 24-well plate (3422, Corning, Corning, NY, USA) at 37 °C, and put 100 ul dPBS in the lower cells.

Techniques: Immunostaining, Staining